AB85754

Anti-DHX30 antibody

Name: Anti-DHX30 antibody (ab85754) | Abcam
Brand: Abcam Limited
SKU: AB85754
Price: 520 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal DHX30 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DHX30 aa 1100 to C-terminus.

View Alternative Names

DDX30, KIAA0890, DHX30, ATP-dependent RNA helicase DHX30, DEAH box protein 30

2 Images

Unknown

Western blot - Anti-DHX30 antibody (AB85754)

All lanes:

Western blot - Anti-DHX30 antibody (ab85754) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 134 kDa

Observed band size: 110 kDa,134 kDa,180 kDa

false

Exposure time: 3min

Unknown

Immunoprecipitation - Anti-DHX30 antibody (AB85754)

Detection of Human DHX3 by Western Blot of Immunprecipitate.
All lanes : ab85754 at 0.4µg/ml staining DHX3 in HeLa whole cell lysates immunoprecipitated at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using the following antibodies :
Lane 1 : ab85754
Lane 2 : Control IgG
Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-DHX30 antibody (ab85754)

Predicted band size: 134 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human DHX30 aa 1100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q7L2E3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab85754 was affinity purified using an epitope specific to DHX30 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DHX30 also known as DExH-Box Helicase 30 is a member of the DEAD-box helicase family and plays a role in RNA metabolism. This protein has a mass of approximately 132 kDa and is widely expressed across various tissues with notable abundance in the brain and testes. Its core mechanical function includes unwinding RNA structures which is critical for the assembly and remodeling of ribonucleoprotein complexes. By doing so DHX30 facilitates various steps in RNA processing including transcription splicing and translation initiation.

Biological function summary

Within cellular processes DHX30 contributes to mitochondrial function and protein synthesis by participating in RNA splicing and translation. It forms part of larger ribonucleoprotein complexes that regulate RNA dynamics. The protein interacts with other cellular components to coordinate the expression of genes involved in cellular growth and metabolism. Its presence in the mitochondrial matrix indicates a specific role in maintaining mitochondrial health and efficient energy production.

Pathways

The DHX30 protein influences cellular activities related to RNA-binding and translation regulation. It is deeply integrated within the translation initiation pathway working alongside RNA-binding proteins to regulate gene expression efficiently. Within this context DHX30 associates with proteins such as EIF4A which is involved in initiating translation of mRNA. Moreover DHX30 participates in maintaining proper functioning of the mitochondrial translation machinery ensuring the synthesis of essential mitochondrial proteins that power the cell's energy requirements.

DHX30 has links to intellectual disabilities and neurodegenerative disorders. Variants or deficiencies in DHX30's function can contribute to conditions such as neurodevelopmental disorders which could potentially connect with other proteins like FMRP involved in synaptic function and mental development. Additionally disruptions in DHX30 activity have been observed in certain types of cancers where aberrant RNA processing and gene expression stability are important factors. Understanding these associations may provide insights into the role of DHX30 in maintaining cellular integrity and its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-dependent helicase (PubMed : 29100085). Plays an important role in the assembly of the mitochondrial large ribosomal subunit (PubMed : 25683715, PubMed : 29100085). Required for optimal function of the zinc-finger antiviral protein ZC3HAV1 (By similarity). Associates with mitochondrial DNA (PubMed : 18063578). Involved in nervous system development and differentiation through its involvement in the up-regulation of a number of genes which are required for neurogenesis, including GSC, NCAM1, neurogenin, and NEUROD (By similarity).

See full target information DHX30

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 42:11560-9 PubMed25249620

2014

FBXL5-mediated degradation of single-stranded DNA-binding protein hSSB1 controls DNA damage response.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Wei Chen,Bin Liu,Nai-Wang Tang,Yun-Hua Xu,Xiang-Yun Ye,Zi-Ming Li,Xiao-Min Niu,Sheng-Ping Shen,Shun Lu,Ling Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DHX30 antibody

Western blot - Anti-DHX30 antibody (AB85754)

Immunoprecipitation - Anti-DHX30 antibody (AB85754)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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