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AB235100

Anti-DHX32 antibody

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(1 Publication)

Rabbit Polyclonal DHX32 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DHX32 aa 300-500.

View Alternative Names

DDX32, DHX32, Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32, DEAD/H box 32, DEAD/H helicase-like protein 1, DEAH box protein 32, HuDDX32, DHLP1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX32 antibody (AB235100)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX32 antibody (AB235100)

Paraffin-embedded human testis tissue stained for DHX32 using ab235100 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX32 antibody (AB235100)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX32 antibody (AB235100)

Paraffin-embedded human kidney tissue stained for DHX32 using ab235100 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-DHX32 antibody (AB235100)
  • WB

Supplier Data

Western blot - Anti-DHX32 antibody (AB235100)

All lanes:

Western blot - Anti-DHX32 antibody (ab235100) at 1/500 dilution

All lanes:

Mouse liver tissue

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 84 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DHX32 aa 300-500. The exact immunogen used to generate this antibody is proprietary information.

Q7L7V1

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB172483

Anti-SNF2L antibody [EPR12191]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SNF2L antibody [EPR12191] (AB172483)

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Primary Antibodies

AB185546

Anti-WAVE 1 antibody [EPR15021] - N-terminal

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-WAVE 1 antibody [EPR15021] - N-terminal (AB185546)

  • 0
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Primary Antibodies

AB234867

Anti-Use1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Use1 antibody (AB234867)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DHX32 also known as DExH-box helicase 32 is a member of the DEAH-box family of RNA helicases. With a mass of approximately 84 kDa DHX32 plays important roles in unwinding RNA secondary structures facilitating processes like RNA splicing and ribosome biogenesis. This protein is highly expressed in various tissues with notable abundance in lymphoid tissues suggesting its involvement in immune function. Its ATP-dependent RNA helicase activity makes it central to post-transcriptional regulation and RNA metabolism.
Biological function summary

DHX32 contributes to the regulation of RNA-related processes. By being part of larger ribonucleoprotein complexes it influences the maturation and processing of ribosomal RNA pre-mRNA splicing and other RNA metabolic pathways. Its function in these complexes ensures the proper assembly and function of the ribosomes which is essential for protein synthesis. The helicase activity provides the energy necessary for these molecular events directly impacting the efficiency and fidelity of RNA processing.

Pathways

Research has indicated that DHX32 is involved in both the spliceosome assembly pathway and the ribosome biogenesis pathway. Within these pathways DHX32 interacts with proteins such as SF3A1 and U2AF2 in the splicing machinery and nucleophosmin (NPM1) in ribosome assembly. These interactions connect DHX32 to a broader network of proteins and ensure the pathways proceed correctly affecting cellular growth and maintenance processes.

DHX32 has shown associations with lymphomas and leukemias reflecting its expression in lymphoid tissues. Its interaction with proteins like c-Myc a well-known oncogene links DHX32 to the regulation of cellular proliferation and survival. These interactions suggest that dysregulation of DHX32 can contribute to the development of malignancies by altering RNA processing and protein synthesis highlighting its potential as a target for therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information DHX32
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancers 16: PubMed38611046

2024

FXR Agonism with Bile Acid Mimetic Reduces Pre-Clinical Triple-Negative Breast Cancer Burden.

Applications

Unspecified application

Species

Unspecified reactive species

Sydney C Joseph,Samson Eugin Simon,Margaret S Bohm,Minjeong Kim,Madeline E Pye,Boston W Simmons,Dillon G Graves,Stacey M Thomas-Gooch,Ubaid A Tanveer,Jeremiah R Holt,Suriyan Ponnusamy,Laura M Sipe,D Neil Hayes,Katherine L Cook,Ramesh Narayanan,Joseph F Pierre,Liza Makowski
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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