AB72451

Anti-DHX33 antibody

Name: Anti-DHX33 antibody (ab72451) | Abcam
Brand: Abcam Limited
SKU: AB72451
Price: 520 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal DHX33 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human DHX33 aa 650 to C-terminus.

View Alternative Names

DDX33, DHX33, ATP-dependent RNA helicase DHX33, DEAH box protein 33

2 Images

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Western blot - Anti-DHX33 antibody (AB72451)

All lanes:

Western blot - Anti-DHX33 antibody (ab72451) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 79 kDa

Observed band size: 200 kDa,31 kDa,79 kDa

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Exposure time: 3min

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Immunoprecipitation - Anti-DHX33 antibody (AB72451)

Immunoprecipitation of DHX33 from 1mg whole HeLa cells lysate by 3 ug ab72451 followed by WB (20% of IP loaded) :
Lane 1 : ab72451 precipitate

Lane 2 : Control IgG

For blotting immunoprecipitated DHX33, ab72451 was used at 1 ug/ml.

Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-DHX33 antibody (ab72451)

Predicted band size: 79 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human DHX33 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H6R0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Affinity purified using an epitope specific to DHX33 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DEAD-Box Helicase 33 (DHX33) is an important enzyme involved in unwinding RNA. Often known as DDX33 its molecular mass is approximately 81 kDa. DHX33 functions as an ATP-dependent RNA helicase actively participating in the processing of ribosomal RNA (rRNA) during ribosome biogenesis. The expression of DHX33 is found largely in tissue where cell growth is high such as embryonic and cancerous cells. By altering the secondary structure of RNA DHX33 plays a direct role in the modification and maturation of rRNA.

Biological function summary

DHX33 plays a role in cellular processes that involve RNA metabolism. It forms part of a larger ribonucleoprotein complex taking an active role in ribosome assembly. This complex promotes the synthesis of ribosomes which are vital for protein synthesis in cells. Proper functioning of DHX33 is important for maintaining the balance of cell proliferation and differentiation largely due to its involvement in the production and processing of rRNA. DHX33's activity is essential for effective ribosome function which impacts cellular function and growth.

Pathways

DHX33 integrates into the ribosome biogenesis and p53 signaling pathways. Within these it interacts with proteins such as nucleophosmin (NPM1) and nucleolin which are influential in nucleolar structure and function. In the p53 pathway DHX33 influences the cellular stress response by affecting the regulation of the tumor suppressor protein p53. By acting within these pathways DHX33 affects protein synthesis and cell cycle progression linking it to cellular homeostasis and stress response.

DHX33 has associations with cancer and ribosomopathies. Overexpression or mutations of DHX33 can link it to tumorigenesis given its role in uncontrolled cell growth and proliferation. In ribosomopathies alterations in DHX33 function can result in defective ribosome biogenesis impacting cell division and growth. The disruption of DHX33's interaction with NPM1 in particular is observed in several forms of cancer highlighting its potential as a target for cancer therapy.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Implicated in nucleolar organization, ribosome biogenesis, protein synthesis and cytoplasmic dsRNA sensing (By similarity) (PubMed : 21930779, PubMed : 23871209, PubMed : 26100019). Stimulates RNA polymerase I transcription of the 47S precursor rRNA. Associates with ribosomal DNA (rDNA) loci where it is involved in POLR1A recruitment (PubMed : 21930779). In the cytoplasm, promotes elongation-competent 80S ribosome assembly at the late stage of mRNA translation initiation (PubMed : 26100019). Senses cytosolic dsRNA mediating NLRP3 inflammasome formation in macrophages and type I interferon production in myeloid dendritic cells (PubMed : 23871209). Required for NLRP3 activation induced by viral dsRNA and bacterial RNA (PubMed : 23871209). In dendritic cells, required for induction of type I interferon production induced by cytoplasmic dsRNA via the activation of MAPK and NF-kappa-B signaling pathways (By similarity).

See full target information DHX33

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39062881

2024

Multi-Omics Characterization of E3 Regulatory Patterns in Different Cancer Types.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyan Li,Jingting Wan,Shangfu Li,Yun Tang,Yang-Chi-Dung Lin,Jie Ni,Xiaoxuan Cai,Jinhan Yu,Hsien-Da Huang,Tzong-Yi Lee

FEBS open bio 12:864-876 PubMed35147292

2022

Identification of sodium homeostasis genes in Camelus bactrianus by whole transcriptome sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Zhang,Jing Pan,Chunxia Liu,Fanhua Meng,Yanru Zhang,Junwei Cao,Yu Cao,Huanmin Zhou

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DHX33 antibody

Western blot - Anti-DHX33 antibody (AB72451)

Immunoprecipitation - Anti-DHX33 antibody (AB72451)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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