AB182006

Anti-DHX33 antibody [EPR14093]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal DHX33 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

DDX33, DHX33, ATP-dependent RNA helicase DHX33, DEAH box protein 33

1 Images

Supplier Data

Western blot - Anti-DHX33 antibody [EPR14093] (AB182006)

All lanes:

Western blot - Anti-DHX33 antibody [EPR14093] (ab182006) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

Predicted band size: 79 kDa

false

Carrier free

Anti-DHX33 antibody [EPR14093] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14093

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DEAD-Box Helicase 33 (DHX33) is an important enzyme involved in unwinding RNA. Often known as DDX33 its molecular mass is approximately 81 kDa. DHX33 functions as an ATP-dependent RNA helicase actively participating in the processing of ribosomal RNA (rRNA) during ribosome biogenesis. The expression of DHX33 is found largely in tissue where cell growth is high such as embryonic and cancerous cells. By altering the secondary structure of RNA DHX33 plays a direct role in the modification and maturation of rRNA.

Biological function summary

DHX33 plays a role in cellular processes that involve RNA metabolism. It forms part of a larger ribonucleoprotein complex taking an active role in ribosome assembly. This complex promotes the synthesis of ribosomes which are vital for protein synthesis in cells. Proper functioning of DHX33 is important for maintaining the balance of cell proliferation and differentiation largely due to its involvement in the production and processing of rRNA. DHX33's activity is essential for effective ribosome function which impacts cellular function and growth.

Pathways

DHX33 integrates into the ribosome biogenesis and p53 signaling pathways. Within these it interacts with proteins such as nucleophosmin (NPM1) and nucleolin which are influential in nucleolar structure and function. In the p53 pathway DHX33 influences the cellular stress response by affecting the regulation of the tumor suppressor protein p53. By acting within these pathways DHX33 affects protein synthesis and cell cycle progression linking it to cellular homeostasis and stress response.

DHX33 has associations with cancer and ribosomopathies. Overexpression or mutations of DHX33 can link it to tumorigenesis given its role in uncontrolled cell growth and proliferation. In ribosomopathies alterations in DHX33 function can result in defective ribosome biogenesis impacting cell division and growth. The disruption of DHX33's interaction with NPM1 in particular is observed in several forms of cancer highlighting its potential as a target for cancer therapy.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Implicated in nucleolar organization, ribosome biogenesis, protein synthesis and cytoplasmic dsRNA sensing (By similarity) (PubMed : 21930779, PubMed : 23871209, PubMed : 26100019). Stimulates RNA polymerase I transcription of the 47S precursor rRNA. Associates with ribosomal DNA (rDNA) loci where it is involved in POLR1A recruitment (PubMed : 21930779). In the cytoplasm, promotes elongation-competent 80S ribosome assembly at the late stage of mRNA translation initiation (PubMed : 26100019). Senses cytosolic dsRNA mediating NLRP3 inflammasome formation in macrophages and type I interferon production in myeloid dendritic cells (PubMed : 23871209). Required for NLRP3 activation induced by viral dsRNA and bacterial RNA (PubMed : 23871209). In dendritic cells, required for induction of type I interferon production induced by cytoplasmic dsRNA via the activation of MAPK and NF-kappa-B signaling pathways (By similarity).

See full target information DHX33

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 135: PubMed34878135

2022

The APC/C targets the Cep152-Cep63 complex at the centrosome to regulate mitotic spindle assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Tischer,Jing Yang,David Barford

Genes & genomics 43:857-868 PubMed33843021

2021

Long non-coding RNA HOTAIR promotes hepatocellular carcinoma progression by regulating miR-526b-3p/DHX33 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Liu,Guoqing Ouyang,Wuchang Lu,Huiming Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DHX33 antibody [EPR14093]

Western blot - Anti-DHX33 antibody [EPR14093] (AB182006)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise