AB222244

Anti-DHX35 antibody

Name: Anti-DHX35 antibody (ab222244) | Abcam
Brand: Abcam Limited
SKU: AB222244

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(1 Publication)

Rabbit Polyclonal DHX35 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DHX35 aa 600 to C-terminus.

View Alternative Names

C20orf15, DDX35, DHX35, Probable ATP-dependent RNA helicase DHX35, DEAH box protein 35

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-DHX35 antibody (AB222244)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DHX35 antibody (AB222244)

4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RH-30 cells stained for DHX35 (green) using ab222244 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DHX35 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H5Z1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DHX35 also known as DExH-box Helicase 35 is a member of the DEAD box protein family characterized by its Asp-Glu-Ala-Asp (DEAD) motif. This protein has a molecular mass of approximately 82 kDa. DHX35 functions mechanically as an ATP-dependent RNA helicase unfurling RNA secondary structures during processes such as transcription and translation. It is widely expressed in various tissues but notable expression occurs in the liver and testis. These helicases are important for manipulating RNA morphology to facilitate cellular processes.

Biological function summary

DHX35 has roles in RNA metabolism influencing processes such as RNA splicing and ribosome biogenesis. It doesn't operate alone; instead it integrates into intricate complexes with other proteins to regulate RNA dynamics. Its activity is important for proper RNA association with ribonucleoprotein complexes impacting RNA-mediated gene expression regulation and maintenance of cellular homeostasis.

Pathways

DHX35 contributes significantly to the RNA processing pathways particularly the RNA splicing pathway. Its interactions with proteins such as small nuclear ribonucleoproteins (snRNPs) highlight its participation in the spliceosome complex. Furthermore DHX35 also plays a part in ribosome biogenesis pathways linking to proteins such as nucleolin which assist in rRNA transcription and ribosomal assembly.

DHX35 shows connections to genetic disorders like Male Infertility where dysregulation impacts sperm development and function. Additionally variations in DHX35 expression or function have associations with hepatocellular carcinoma suggesting a role in tumor biology. These associations stem from interactions with other proteins like DDX3X which also influence RNA processing in cancer and reproductive health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in pre-mRNA splicing.

See full target information DHX35

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 12: PubMed38540147

2024

Circulating Exosomes from Septic Mice Activate NF-κB/MIR17HG Pathway in Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Shao-Chun Wu,Cheng-Shyuan Rau,Yi-Chan Wu,Chia-Jung Wu,Chia-Wen Tsai,Lien-Hung Huang,Chia-Wei Lin,Tsu-Hsiang Lu,Ming-Yu Yang,Ching-Hua Hsieh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DHX35 antibody

Immunocytochemistry/ Immunofluorescence - Anti-DHX35 antibody (AB222244)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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