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AB235366

Anti-DHX35 antibody

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(2 Publications)

Rabbit Polyclonal DHX35 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DHX35 aa 1-300.

View Alternative Names

C20orf15, DDX35, DHX35, Probable ATP-dependent RNA helicase DHX35, DEAH box protein 35

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX35 antibody (AB235366)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX35 antibody (AB235366)

Paraffin-embedded human brain tissue stained for DHX35 using ab235366 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX35 antibody (AB235366)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX35 antibody (AB235366)

Paraffin-embedded human testis tissue stained for DHX35 using ab235366 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-DHX35 antibody (AB235366)
  • WB

Supplier Data

Western blot - Anti-DHX35 antibody (AB235366)

All lanes:

Western blot - Anti-DHX35 antibody (ab235366) at 1/1000 dilution

Lane 1:

Mouse kidney lysate

Lane 2:

Mouse liver lysate

Lane 3:

K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 79 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DHX35 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9H5Z1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DHX35 also known as DExH-box Helicase 35 is a member of the DEAD box protein family characterized by its Asp-Glu-Ala-Asp (DEAD) motif. This protein has a molecular mass of approximately 82 kDa. DHX35 functions mechanically as an ATP-dependent RNA helicase unfurling RNA secondary structures during processes such as transcription and translation. It is widely expressed in various tissues but notable expression occurs in the liver and testis. These helicases are important for manipulating RNA morphology to facilitate cellular processes.
Biological function summary

DHX35 has roles in RNA metabolism influencing processes such as RNA splicing and ribosome biogenesis. It doesn't operate alone; instead it integrates into intricate complexes with other proteins to regulate RNA dynamics. Its activity is important for proper RNA association with ribonucleoprotein complexes impacting RNA-mediated gene expression regulation and maintenance of cellular homeostasis.

Pathways

DHX35 contributes significantly to the RNA processing pathways particularly the RNA splicing pathway. Its interactions with proteins such as small nuclear ribonucleoproteins (snRNPs) highlight its participation in the spliceosome complex. Furthermore DHX35 also plays a part in ribosome biogenesis pathways linking to proteins such as nucleolin which assist in rRNA transcription and ribosomal assembly.

DHX35 shows connections to genetic disorders like Male Infertility where dysregulation impacts sperm development and function. Additionally variations in DHX35 expression or function have associations with hepatocellular carcinoma suggesting a role in tumor biology. These associations stem from interactions with other proteins like DDX3X which also influence RNA processing in cancer and reproductive health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in pre-mRNA splicing.
See full target information DHX35
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 20:e1012379 PubMed39037956

2024

The RNA helicase DHX35 functions as a co-sensor for RIG-I-mediated innate immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Qiao,Shan Zhu,Ning Yang,Shan-Shan Zou,Bao Gao,Jing Wu,Chunyan Liu,Xiaoping Li,Yong-Jun Liu,Jingtao Chen

Journal of clinical medicine 10: PubMed33925488

2021

Combined Inhibition of TGF-β1-Induced EMT and PD-L1 Silencing Re-Sensitizes Hepatocellular Carcinoma to Sorafenib Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Ritu Shrestha,Prashanth Prithviraj,Kim R Bridle,Darrell H G Crawford,Aparna Jayachandran
View all publications
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