JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB250087

Anti-DHX35 antibody [EPR12383] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal DHX35 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C20orf15, DDX35, DHX35, Probable ATP-dependent RNA helicase DHX35, DEAH box protein 35

1 Images
Western blot - Anti-DHX35 antibody [EPR12383] - BSA and Azide free (AB250087)
  • WB

Supplier Data

Western blot - Anti-DHX35 antibody [EPR12383] - BSA and Azide free (AB250087)

This data was developed using ab179442, the same antibody clone in a different buffer formulation.

All lanes:

(ab179242) at 1/1000 dilution

Lane 1:

HeLa cell line lysate at 10 µg

Lane 2:

Jurkat cell line lysate at 10 µg

Lane 3:

K562 cell line lysate at 10 µg

Lane 4:

WI38 cell line lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/500 dilution

Predicted band size: 79 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12383

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

ab250087 is the carrier-free version of ab179442.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DHX35 also known as DExH-box Helicase 35 is a member of the DEAD box protein family characterized by its Asp-Glu-Ala-Asp (DEAD) motif. This protein has a molecular mass of approximately 82 kDa. DHX35 functions mechanically as an ATP-dependent RNA helicase unfurling RNA secondary structures during processes such as transcription and translation. It is widely expressed in various tissues but notable expression occurs in the liver and testis. These helicases are important for manipulating RNA morphology to facilitate cellular processes.
Biological function summary

DHX35 has roles in RNA metabolism influencing processes such as RNA splicing and ribosome biogenesis. It doesn't operate alone; instead it integrates into intricate complexes with other proteins to regulate RNA dynamics. Its activity is important for proper RNA association with ribonucleoprotein complexes impacting RNA-mediated gene expression regulation and maintenance of cellular homeostasis.

Pathways

DHX35 contributes significantly to the RNA processing pathways particularly the RNA splicing pathway. Its interactions with proteins such as small nuclear ribonucleoproteins (snRNPs) highlight its participation in the spliceosome complex. Furthermore DHX35 also plays a part in ribosome biogenesis pathways linking to proteins such as nucleolin which assist in rRNA transcription and ribosomal assembly.

DHX35 shows connections to genetic disorders like Male Infertility where dysregulation impacts sperm development and function. Additionally variations in DHX35 expression or function have associations with hepatocellular carcinoma suggesting a role in tumor biology. These associations stem from interactions with other proteins like DDX3X which also influence RNA processing in cancer and reproductive health.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

May be involved in pre-mRNA splicing.
See full target information DHX35
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 22:74 PubMed40069860

2025

Inhibition of histone deacetylase 6 alleviates neuropathic pain via direct regulating post-translation of spinal STAT3 and decreasing downstream C-C Motif Chemokine Ligand 7 synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhexi Chi,Bo Lu,Rongjun Liu,Chen Pan,Bo Meng,Xiuzhong Xing,Hui Yuan,Xuewei Wu,Yushan Chen,Yuxuan Ren,Wenwei Wu,Mengmeng Miao,Junping Chen,Xiaowei Chen
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB230392

Anti-DHTKD1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHTKD1 antibody (AB230392)

  • 0
  • 0
Primary Antibodies

AB109012

Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

KO Validated|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB109012)

  • 5
  • 15
Primary Antibodies

AB4729

Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729)

  • 5
  • 85
Primary Antibodies

AB222313

Anti-DCP1B antibody

Immunocytochemistry/ Immunofluorescence - Anti-DCP1B antibody (AB222313)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com