Rabbit Polyclonal DHX36 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human DHX36.
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Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human DHX36. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H2U1
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Multifunctional ATP-dependent helicase that unwinds G-quadruplex (G4) structures (PubMed:16150737, PubMed:18854321, PubMed:20472641, PubMed:21586581). Plays a role in many biological processes such as genomic integrity, gene expression regulations and as a sensor to initiate antiviral responses (PubMed:14731398, PubMed:18279852, PubMed:21993297, PubMed:22238380, PubMed:25579584). G4 structures correspond to helical structures containing guanine tetrads (By similarity). Binds with high affinity to and unwinds G4 structures that are formed in nucleic acids (G4-ADN and G4-RNA) (PubMed:16150737, PubMed:18842585, PubMed:20472641, PubMed:21586581, PubMed:24369427, PubMed:26195789). Plays a role in genomic integrity (PubMed:22238380). Converts the G4-RNA structure present in telomerase RNA template component (TREC) into a double-stranded RNA to promote P1 helix formation that acts as a template boundary ensuring accurate reverse transcription (PubMed:20472641, PubMed:21149580, PubMed:21846770, PubMed:22238380, PubMed:24151078, PubMed:25579584). Plays a role in transcriptional regulation (PubMed:21586581, PubMed:21993297). Resolves G4-DNA structures in promoters of genes, such as YY1, KIT/c-kit and ALPL and positively regulates their expression (PubMed:21993297). Plays a role in post-transcriptional regulation (PubMed:27940037). Unwinds a G4-RNA structure located in the 3'-UTR polyadenylation site of the pre-mRNA TP53 and stimulates TP53 pre-mRNA 3'-end processing in response to ultraviolet (UV)-induced DNA damage (PubMed:27940037). Binds to the precursor-microRNA-134 (pre-miR-134) terminal loop and regulates its transport into the synapto-dendritic compartment (By similarity). Involved in the pre-miR-134-dependent inhibition of target gene expression and the control of dendritic spine size (By similarity). Plays a role in the regulation of cytoplasmic mRNA translation and mRNA stability (PubMed:24369427, PubMed:26489465). Binds to both G4-RNA structures and alternative non-quadruplex-forming sequence within the 3'-UTR of the PITX1 mRNA regulating negatively PITX1 protein expression (PubMed:24369427). Binds to both G4-RNA structure in the 5'-UTR and AU-rich elements (AREs) localized in the 3'-UTR of NKX2-5 mRNA to either stimulate protein translation or induce mRNA decay in an ELAVL1-dependent manner, respectively (PubMed:26489465). Binds also to ARE sequences present in several mRNAs mediating exosome-mediated 3'-5' mRNA degradation (PubMed:14731398, PubMed:18279852). Involved in cytoplasmic urokinase-type plasminogen activator (uPA) mRNA decay (PubMed:14731398). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1 (By similarity). Required for early embryonic development and hematopoiesis. Involved in the regulation of cardioblast differentiation and proliferation during heart development. Involved in spermatogonia differentiation. May play a role in ossification (By similarity).
Alternative names
ATP-dependent DNA/RNA helicase DHX36, DEAD/H box polypeptide 36, DEAH-box protein 36, G4-resolvase-1, MLE-like protein 1, RNA helicase associated with AU-rich element protein, G4R1, KIAA1488, MLEL1, RHAU, DDX36, DHX36
Recommended products
Rabbit Polyclonal DHX36 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human DHX36.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human DHX36. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H2U1
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DHX36 also known as RHAU or G4R1 is a member of the DEAH-box family of RNA helicases. It has an estimated molecular mass of approximately 115 kDa. The protein exhibits robust expression in various tissues including the heart skeletal muscle and brain. Mechanically DHX36 plays a role in resolving RNA G-quadruplexes and DNA G-quadruplexes which are secondary structures that can form in nucleic acids. Its ability to unwind these structures is critical for the regulation of gene expression and maintenance of genomic stability.
- Biological function summary
DHX36 acts as an RNA helicase and is essential in the regulation of translation and transcription. It plays a significant role in binding G-quadruplex structures within the 5' untranslated region of mRNAs. DHX36 also forms part of larger protein complexes that regulate ribosome assembly and function. This protein helps ensure proper ribosomal RNA processing facilitating efficient protein synthesis. Through its involvement in these complexes DHX36 aids in the regulation of cellular stress responses and apoptosis.
- Pathways
DHX36 significantly contributes to several cellular processes notably the regulation of transcription and translation. It actively participates in the Wnt signaling pathway influencing the stability of target mRNAs. DHX36 also interacts with proteins like FTO and NPM1 that are known to be involved in mRNA processing. These interactions and pathway linkages highlight its role in controlling gene expression and cellular growth.
- Associated diseases and disorders
DHX36 has been linked to cardiac dysfunctions and neurological disorders. Its improper functioning has been associated with arrhythmias due to its role in cardiac gene expression regulation. In neurological contexts DHX36 disruptions may contribute to various cognitive disorders acting through its impact on synaptic protein synthesis. In these pathological conditions proteins such as TDP-43 and FUS are affected emphasizing the importance of DHX36 in maintaining cellular homeostasis and preventing disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-DHX36 antibody (ab226813)
All lanes: Western blot - Anti-DHX36 antibody (ab226813) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 30 µg
Lane 2: A431 (human epidermoid carcinoma cell line) cell lysate at 30 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 30 µg
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 30 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 115 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX36 antibody (ab226813)
Paraffin-embedded mouse testis tissue stained for DHX36 using ab226813 at 1/400 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX36 antibody (ab226813)
Paraffin-embedded rat brain tissue stained for DHX36 using ab226813 at 1/400 dilution in immunohistochemical analysis.
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