AB223564

Anti-DHX36 antibody [EPR20868-12]

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal DHX36 antibody. Suitable for IP, WB and reacts with Mouse samples.

View Alternative Names

Ddx36, Kiaa1488, Mlel1, Dhx36, ATP-dependent DNA/RNA helicase DHX36, DEAD/H box polypeptide 36, DEAH box protein 36, MLE-like protein 1, RNA helicase associated with AU-rich element ARE

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Immunoprecipitation - Anti-DHX36 antibody [EPR20868-12] (AB223564)

DHX36 was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab223564 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab223564 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10ug

Lane 2 : ab223564 IP in RAW 264.7 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab223564 in RAW 264.7 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes

All lanes:

Immunoprecipitation - Anti-DHX36 antibody [EPR20868-12] (ab223564)

Predicted band size: 115 kDa

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Lab

Western blot - Anti-DHX36 antibody [EPR20868-12] (AB223564)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lysates were kindly provided by Dr Zhongzhou Yang.

ab223564 was shown to specifically react with DHX36 in wild-type mouse embryo heart tissue as signal was weaken in DHX36 knock-down tissue. Wild-type and DHX36 knock-down samples were subjected to SDS-PAGE. ab223564 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200, 000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/20, 000 dilution and 1/100, 000 dilution respectively for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

Exposure time : 15 seconds

All lanes:

Western blot - Anti-DHX36 antibody [EPR20868-12] (ab223564) at 1/1000 dilution

Lane 1:

Wild-type mouse embryo heart tissue lysate at 20 µg

Lane 2:

DHX36 knock-down mouse embryo heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 115 kDa

Observed band size: 114 kDa

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Lab

Western blot - Anti-DHX36 antibody [EPR20868-12] (AB223564)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time :

Lanes 1-5 : 3 minutes;

Lane 6 : 15 seconds.

All lanes:

Western blot - Anti-DHX36 antibody [EPR20868-12] (ab223564) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 4:

Mouse lung tissue lysate at 20 µg

Lane 5:

Mouse brain tissue lysate at 20 µg

Lane 6:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 115 kDa

Observed band size: 114 kDa

false

Carrier free

Anti-DHX36 antibody [EPR20868-12] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20868-12

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "Use at 1:30 dilution (2μg in 0.35mg lysates).", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DHX36 also known as RHAU or G4R1 is a member of the DEAH-box family of RNA helicases. It has an estimated molecular mass of approximately 115 kDa. The protein exhibits robust expression in various tissues including the heart skeletal muscle and brain. Mechanically DHX36 plays a role in resolving RNA G-quadruplexes and DNA G-quadruplexes which are secondary structures that can form in nucleic acids. Its ability to unwind these structures is critical for the regulation of gene expression and maintenance of genomic stability.

Biological function summary

DHX36 acts as an RNA helicase and is essential in the regulation of translation and transcription. It plays a significant role in binding G-quadruplex structures within the 5' untranslated region of mRNAs. DHX36 also forms part of larger protein complexes that regulate ribosome assembly and function. This protein helps ensure proper ribosomal RNA processing facilitating efficient protein synthesis. Through its involvement in these complexes DHX36 aids in the regulation of cellular stress responses and apoptosis.

Pathways

DHX36 significantly contributes to several cellular processes notably the regulation of transcription and translation. It actively participates in the Wnt signaling pathway influencing the stability of target mRNAs. DHX36 also interacts with proteins like FTO and NPM1 that are known to be involved in mRNA processing. These interactions and pathway linkages highlight its role in controlling gene expression and cellular growth.

DHX36 has been linked to cardiac dysfunctions and neurological disorders. Its improper functioning has been associated with arrhythmias due to its role in cardiac gene expression regulation. In neurological contexts DHX36 disruptions may contribute to various cognitive disorders acting through its impact on synaptic protein synthesis. In these pathological conditions proteins such as TDP-43 and FUS are affected emphasizing the importance of DHX36 in maintaining cellular homeostasis and preventing disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Multifunctional ATP-dependent helicase that unwinds G-quadruplex (G4) structures (PubMed : 25611385). Plays a role in many biological processes such as genomic integrity, gene expression regulations and as a sensor to initiate antiviral responses (PubMed : 21590736, PubMed : 21703541). G4 structures correspond to helical structures containing guanine tetrads (By similarity). Binds with high affinity to and unwinds G4 structures that are formed in nucleic acids (G4-DNA and G4-RNA) (By similarity). Plays a role in genomic integrity (By similarity). Converts the G4-RNA structure present in telomerase RNA template component (TREC) into a double-stranded RNA to promote P1 helix formation that acts as a template boundary ensuring accurate reverse transcription (By similarity). Plays a role in transcriptional regulation. Resolves G4-DNA structures in promoters of genes, such as YY1, KIT/c-kit and ALPL and positively regulates their expression (By similarity) (PubMed : 25611385). Plays a role in post-transcriptional regulation (By similarity). Unwinds a G4-RNA structure located in the 3'-UTR polyadenylation site of the pre-mRNA TP53 and stimulates TP53 pre-mRNA 3'-end processing in response to ultraviolet (UV)-induced DNA damage (By similarity). Binds to the precursor-microRNA-134 (pre-miR-134) terminal loop and regulates its transport into the synapto-dendritic compartment (By similarity). Involved in the pre-miR-134-dependent inhibition of target gene expression and the control of dendritic spine size (By similarity). Plays a role in the regulation of cytoplasmic mRNA translation and mRNA stability (By similarity). Binds to both G4-RNA structures and alternative non-quadruplex-forming sequence within the 3'-UTR of the PITX1 mRNA regulating negatively PITX1 protein expression (By similarity). Binds to both G4-RNA structure in the 5'-UTR and AU-rich elements (AREs) localized in the 3'-UTR of NKX2-5 mRNA to either stimulate protein translation or induce mRNA decay in an ELAVL1-dependent manner, respectively (By similarity). Also binds to ARE sequences present in several mRNAs mediating exosome-mediated 3'-5' mRNA degradation (By similarity). Involved in cytoplasmic urokinase-type plasminogen activator (uPA) mRNA decay (By similarity). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines via the adapter molecule TICAM1 (PubMed : 21703541). Required for the early embryonic development and hematopoiesis (PubMed : 22422825). Involved in the regulation of cardioblast differentiation and proliferation during heart development (PubMed : 26489465). Involved in spermatogonia differentiation (PubMed : 25611385). May play a role in ossification (PubMed : 21590736).

See full target information Dhx36

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DHX36 antibody [EPR20868-12]

Immunoprecipitation - Anti-DHX36 antibody [EPR20868-12] (AB223564)

Western blot - Anti-DHX36 antibody [EPR20868-12] (AB223564)

Western blot - Anti-DHX36 antibody [EPR20868-12] (AB223564)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise