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AB70778

Anti-DHX37 antibody

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(2 Publications)

Rabbit Polyclonal DHX37 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

DDX37, KIAA1517, DHX37, Probable ATP-dependent RNA helicase DHX37, DEAH box protein 37

2 Images
Immunoprecipitation - Anti-DHX37 antibody (AB70778)
  • IP

Unknown

Immunoprecipitation - Anti-DHX37 antibody (AB70778)

Detection of DHX37 by Western Blot of Immunprecipitate. ab70778 at 1µg/ml staining DHX37 in HeLa whole cell lysate immunoprecipitated using ab70778 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-DHX37 antibody (ab70778)

Predicted band size: 130 kDa

false

Western blot - Anti-DHX37 antibody (AB70778)
  • WB

Supplier Data

Western blot - Anti-DHX37 antibody (AB70778)

All lanes:

Western blot - Anti-DHX37 antibody (ab70778) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 130 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab70778 was affinity purified using an epitope specific to DHX37 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DHX37 protein also known as DEAD-box helicase 37 functions mechanically as an ATP-dependent RNA helicase. It belongs to the DEAD-box family of proteins involved in altering RNA secondary structure. DHX37 has a molecular mass of approximately 124 kDa. One can detect its expression in the cytoplasm and nucleus across various tissues with high levels noted in the testes and brain indicating its importance in specific cellular activities.
Biological function summary

DHX37 plays a pivotal role in ribosome biogenesis and RNA processing events. It participates in the assembly of pre-ribosomal particles a complex essential for maturing ribosomal RNA. This activity implies DHX37's involvement in the early stages of ribosome formation impacting protein synthesis broadly across eukaryotic cells. Its helicase activity is necessary for unwinding RNA duplexes facilitating the progression of these fundamental biological processes.

Pathways

DHX37 emerges as significant within the ribosome biogenesis pathway. This pathway chart important steps for the synthesis and assembly of ribosomes which are key for cellular translation machinery. Additionally DHX37 interacts with proteins like NOP56 and NOP58 in the same pathway highlighting its relational involvement and integral role in facilitating concerted actions among participating molecules.

DHX37's dysfunction associates with intellectual disabilities and male infertility. Mutations in this protein can lead to compromised RNA processing and ribosome assembly affecting neural development and gametogenesis. It shows interactions with proteins such as X-linked mental retardation associated proteins which further ties its activity to cognitive impairment conditions. Understanding the molecular interplay of DHX37 in these contexts aids in the exploration of therapeutic avenues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-binding RNA helicase that plays a role in maturation of the small ribosomal subunit in ribosome biogenesis (PubMed : 30582406). Required for the release of the U3 snoRNP from pre-ribosomal particles (PubMed : 30582406). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797). Plays a role in early testis development (PubMed : 31287541, PubMed : 31337883). Probably also plays a role in brain development (PubMed : 31256877).
See full target information DHX37
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

mBio 11: PubMed32156816

2020

RNA Binding Motif Protein RBM45 Regulates Expression of the 11-Kilodalton Protein of Parvovirus B19 through Binding to Novel Intron Splicing Enhancers.

Applications

Unspecified application

Species

Unspecified reactive species

Jianke Wang,Safder S Ganaie,Fang Cheng,Peng Xu,Kang Ning,Xiaomei Wang,Steve Kleiboeker,Shipeng Cheng,Jianming Qiu

RNA (New York, N.Y.) 25:685-701 PubMed30910870

2019

Molecular mechanism of the RNA helicase DHX37 and its activation by UTP14A in ribosome biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Franziska M Boneberg,Tobias Brandmann,Lena Kobel,Jasmin van den Heuvel,Katja Bargsten,Lukas Bammert,Ulrike Kutay,Martin Jinek
View all publications
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