AB70588

Anti-DHX8/prp22 antibody

Name: Anti-DHX8/prp22 antibody (ab70588) | Abcam
Brand: Abcam Limited
SKU: AB70588
Rating: 3 (2 reviews)

(2 Reviews)

(1 Publication)

Rabbit Polyclonal DHX8/prp22 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DHX8 aa 300-400.

View Alternative Names

DDX8, DHX8, ATP-dependent RNA helicase DHX8, DEAH box protein 8, RNA helicase HRH1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX8/prp22 antibody (AB70588)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX8/prp22 antibody (AB70588)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse teratoma (right) tissues labelling DHX8/prp22 with ab70588 at 1/200 (1μg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-DHX8/prp22 antibody (AB70588)

Immunoprecipitation of DHX8/prp22 from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab70588 at 3 μg/mg lysate for IP and at 1 μg/ml for subsequent WB detection.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-DHX8/prp22 antibody (ab70588)

Predicted band size: 139 kDa

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Unknown

Western blot - Anti-DHX8/prp22 antibody (AB70588)

All lanes:

Western blot - Anti-DHX8/prp22 antibody (ab70588) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 139 kDa

Observed band size: 117 kDa,150 kDa,65 kDa,<30 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human DHX8 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

Q14562

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-4 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Tilapia": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DHX8 also known as Prp22 is a protein involved in RNA processing. Mechanically it functions as an ATP-dependent RNA helicase. This protein facilitates the unwinding of RNA structures during the spliceosome cycle essential for accurate splicing of pre-mRNA to mature mRNA. The molecular weight of DHX8/Prp22 is approximately 140 kDa. It is widely expressed in animal cells particularly in tissues with high levels of RNA processing activity such as the liver and testis.

Biological function summary

DHX8/Prp22 plays a critical role in pre-mRNA splicing. It is a component of the spliceosome a large ribonucleoprotein complex responsible for excising introns from pre-mRNA. Within this complex DHX8/Prp22 helps in the transition to the second step of splicing by promoting the release of spliced mRNA and dissociation of proteins and snRNPs. The activity of DHX8/Prp22 ensures proper removal of introns and prevents exon skipping contributing to the fidelity of gene expression.

Pathways

The DHX8/Prp22 is an integral part of the cellular RNA splicing pathway. It connects with the spliceosomal cycle and influences mRNA maturation which is essential for proper gene expression and cellular function. This protein has associations with other helicases like DHX15 which also work in mRNA splicing pathways indicating a complex interplay of helicases in gene expression regulation. Splicing impacts various cellular processes linking DHX8/Prp22 to larger networks of gene expression regulation.

DHX8/Prp22 is linked to certain genetic diseases where splicing errors occur such as retinitis pigmentosa and some cancers. Improper functioning of DHX8/Prp22 can lead to aberrant splicing resulting in the production of dysfunctional proteins. This helicase interacts with other splicing factors such as SF3B1 mutations of which are found in these conditions highlighting the importance of spliceosome integrity in disease prevention. Understanding DHX8/Prp22's role in these disorders can lead to potential therapeutic strategies targeting splicing mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 11991638, PubMed : 28076346, PubMed : 28502770). Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome (PubMed : 8608946).

See full target information DHX8

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Learning & memory (Cold Spring Harbor, N.Y.) 24:199-209 PubMed28416631

2017

Mutation of neuron-specific chromatin remodeling subunit BAF53b: rescue of plasticity and memory by manipulating actin remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Annie Vogel Ciernia,Enikö A Kramár,Dina P Matheos,Robbert Havekes,Thekla J Hemstedt,Christophe N Magnan,Keith Sakata,Ashley Tran,Soraya Azzawi,Alberto Lopez,Richard Dang,Weisheng Wang,Brian Trieu,Joyce Tong,Ruth M Barrett,Rebecca J Post,Pierre Baldi,Ted Abel,Gary Lynch,Marcelo A Wood

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DHX8/prp22 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX8/prp22 antibody (AB70588)

Immunoprecipitation - Anti-DHX8/prp22 antibody (AB70588)

Western blot - Anti-DHX8/prp22 antibody (AB70588)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise