AB181074

Anti-DHX8/prp22 antibody [EPR9146(2)]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal DHX8/prp22 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

DDX8, DHX8, ATP-dependent RNA helicase DHX8, DEAH box protein 8, RNA helicase HRH1

2 Images

Lab

Western blot - Anti-DHX8/prp22 antibody [EPR9146(2)] (AB181074)

All lanes:

Western blot - Anti-DHX8/prp22 antibody [EPR9146(2)] (ab181074) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 15 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 139 kDa

Observed band size: 139 kDa

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Supplier Data

Western blot - Anti-DHX8/prp22 antibody [EPR9146(2)] (AB181074)

All lanes:

Western blot - Anti-DHX8/prp22 antibody [EPR9146(2)] (ab181074) at 1/20000 dilution

All lanes:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 139 kDa

false

Exposure time: 3min

Carrier free

Anti-DHX8/prp22 antibody [EPR9146(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9146(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DHX8 also known as Prp22 is a protein involved in RNA processing. Mechanically it functions as an ATP-dependent RNA helicase. This protein facilitates the unwinding of RNA structures during the spliceosome cycle essential for accurate splicing of pre-mRNA to mature mRNA. The molecular weight of DHX8/Prp22 is approximately 140 kDa. It is widely expressed in animal cells particularly in tissues with high levels of RNA processing activity such as the liver and testis.

Biological function summary

DHX8/Prp22 plays a critical role in pre-mRNA splicing. It is a component of the spliceosome a large ribonucleoprotein complex responsible for excising introns from pre-mRNA. Within this complex DHX8/Prp22 helps in the transition to the second step of splicing by promoting the release of spliced mRNA and dissociation of proteins and snRNPs. The activity of DHX8/Prp22 ensures proper removal of introns and prevents exon skipping contributing to the fidelity of gene expression.

Pathways

The DHX8/Prp22 is an integral part of the cellular RNA splicing pathway. It connects with the spliceosomal cycle and influences mRNA maturation which is essential for proper gene expression and cellular function. This protein has associations with other helicases like DHX15 which also work in mRNA splicing pathways indicating a complex interplay of helicases in gene expression regulation. Splicing impacts various cellular processes linking DHX8/Prp22 to larger networks of gene expression regulation.

DHX8/Prp22 is linked to certain genetic diseases where splicing errors occur such as retinitis pigmentosa and some cancers. Improper functioning of DHX8/Prp22 can lead to aberrant splicing resulting in the production of dysfunctional proteins. This helicase interacts with other splicing factors such as SF3B1 mutations of which are found in these conditions highlighting the importance of spliceosome integrity in disease prevention. Understanding DHX8/Prp22's role in these disorders can lead to potential therapeutic strategies targeting splicing mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 11991638, PubMed : 28076346, PubMed : 28502770). Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome (PubMed : 8608946).

See full target information DHX8

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Clinical and experimental pharmacology & physiology 51:e13916 PubMed39155151

2024

Attenuation of renal fibrosis in mice due to lack of bombesin receptor-activated protein homologue.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Peng,Hui Wang,Jiaoyun Zheng,Hui Chen,Jie Wang,Horst Christian Weber,Lin Yuan,Xiaoqun Qin,Yang Xiang,Chi Liu,Ming Ji,Huijun Liu,Xiangping Qu

eLife 12: PubMed38488852

2024

Metabolic reprogramming of cancer cells by JMJD6-mediated pre-mRNA splicing associated with therapeutic response to splicing inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Carolyn M Jablonowski,Waise Quarni,Shivendra Singh,Haiyan Tan,Dhanushka Hewa Bostanthirige,Hongjian Jin,Jie Fang,Ti-Cheng Chang,David Finkelstein,Ji-Hoon Cho,Dongli Hu,Vishwajeeth Pagala,Sadie Miki Sakurada,Shondra M Pruett-Miller,Ruoning Wang,Andrew Murphy,Kevin Freeman,Junmin Peng,Andrew M Davidoff,Gang Wu,Jun Yang

Frontiers in oncology 12:900671 PubMed35814441

2022

High Levels of DEAH-Box Helicases Relate to Poor Prognosis and Reduction of DHX9 Improves Radiosensitivity of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Chen,Letao Lin,Guanyu Chen,Huzheng Yan,Zhenyu Li,Meigui Xiao,Xu He,Fujun Zhang,Yanling Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DHX8/prp22 antibody [EPR9146(2)]

Western blot - Anti-DHX8/prp22 antibody [EPR9146(2)] (AB181074)

Western blot - Anti-DHX8/prp22 antibody [EPR9146(2)] (AB181074)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise