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AB246344

Anti-DHX8/prp22 antibody [EPR9146(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal DHX8/prp22 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

DDX8, DHX8, ATP-dependent RNA helicase DHX8, DEAH box protein 8, RNA helicase HRH1

2 Images
Western blot - Anti-DHX8/prp22 antibody [EPR9146(2)] - BSA and Azide free (AB246344)
  • WB

Lab

Western blot - Anti-DHX8/prp22 antibody [EPR9146(2)] - BSA and Azide free (AB246344)

This data was developed using ab181074, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DHX8/prp22 antibody [EPR9146(2)] (<a href='/en-us/products/primary-antibodies/dhx8-prp22-antibody-epr91462-ab181074'>ab181074</a>) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 15 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 139 kDa

Observed band size: 139 kDa

false

Western blot - Anti-DHX8/prp22 antibody [EPR9146(2)] - BSA and Azide free (AB246344)
  • WB

Supplier Data

Western blot - Anti-DHX8/prp22 antibody [EPR9146(2)] - BSA and Azide free (AB246344)

This data was developed using ab181074, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DHX8/prp22 antibody [EPR9146(2)] (<a href='/en-us/products/primary-antibodies/dhx8-prp22-antibody-epr91462-ab181074'>ab181074</a>) at 1/20000 dilution

All lanes:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 139 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9146(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab246344 is the carrier-free version of ab181074.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DHX8 also known as Prp22 is a protein involved in RNA processing. Mechanically it functions as an ATP-dependent RNA helicase. This protein facilitates the unwinding of RNA structures during the spliceosome cycle essential for accurate splicing of pre-mRNA to mature mRNA. The molecular weight of DHX8/Prp22 is approximately 140 kDa. It is widely expressed in animal cells particularly in tissues with high levels of RNA processing activity such as the liver and testis.
Biological function summary

DHX8/Prp22 plays a critical role in pre-mRNA splicing. It is a component of the spliceosome a large ribonucleoprotein complex responsible for excising introns from pre-mRNA. Within this complex DHX8/Prp22 helps in the transition to the second step of splicing by promoting the release of spliced mRNA and dissociation of proteins and snRNPs. The activity of DHX8/Prp22 ensures proper removal of introns and prevents exon skipping contributing to the fidelity of gene expression.

Pathways

The DHX8/Prp22 is an integral part of the cellular RNA splicing pathway. It connects with the spliceosomal cycle and influences mRNA maturation which is essential for proper gene expression and cellular function. This protein has associations with other helicases like DHX15 which also work in mRNA splicing pathways indicating a complex interplay of helicases in gene expression regulation. Splicing impacts various cellular processes linking DHX8/Prp22 to larger networks of gene expression regulation.

DHX8/Prp22 is linked to certain genetic diseases where splicing errors occur such as retinitis pigmentosa and some cancers. Improper functioning of DHX8/Prp22 can lead to aberrant splicing resulting in the production of dysfunctional proteins. This helicase interacts with other splicing factors such as SF3B1 mutations of which are found in these conditions highlighting the importance of spliceosome integrity in disease prevention. Understanding DHX8/Prp22's role in these disorders can lead to potential therapeutic strategies targeting splicing mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 11991638, PubMed : 28076346, PubMed : 28502770). Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome (PubMed : 8608946).
See full target information DHX8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 33:698-712 PubMed37662970

2023

Selection of a novel cell-internalizing RNA aptamer specific for CD22 antigen in B cell acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Dario Ruiz-Ciancio,Li-Hsien Lin,Suresh Veeramani,Maya N Barros,Diego Sanchez,Ary Lautaro Di Bartolo,Diego Masone,Paloma H Giangrande,María Belén Mestre,William H Thiel
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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