Rabbit Polyclonal DIAPH1 antibody. Suitable for IHC-P, WB, ICC/IF, IHC-Fr and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human DIAPH1.
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- Journal of molecular medicine (Berlin, Germany) 101:1015-10282023Novel insights into the nervous system affected by prolonged hyperglycemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKamila Zglejc-Waszak,Konark Mukherjee,Agnieszka Korytko,Bogdan Lewczuk,Andrzej Pomianowski,Joanna Wojtkiewicz,Marta Banach,Michał Załęcki,Natalia Nowicka,Julia Jarosławska,Bernard Kordas,Krzysztof Wąsowicz,Judyta K JuranekPubMed 37462767
- Communications biology 6:2802023DIAPH1 mediates progression of atherosclerosis and regulates hepatic lipid metabolism in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Senatus,Lander Egaña-Gorroño,Raquel López-Díez,Sonia Bergaya,Juan Francisco Aranda,Jaume Amengual,Lakshmi Arivazhagan,Michaele B Manigrasso,Gautham Yepuri,Ramesh Nimma,Kaamashri N Mangar,Rollanda Bernadin,Boyan Zhou,Paul F Gugger,Huilin Li,Richard A Friedman,Neil D Theise,Alexander Shekhtman,Edward A Fisher,Ravichandran Ramasamy,Ann Marie SchmidtPubMed 36932214
- Neuropathology : official journal of the Japanese Society of Neuropathology 43:84-942022The receptor for advanced glycation end products and its ligands' expression in OVE26 diabetic sciatic nerve during the development of length-dependent neuropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKamila Zglejc-Waszak,Ann Marie Schmidt,Judyta K JuranekPubMed 35915909
- International journal of molecular sciences 22:2021Cigarette Smoke Condensate Exposure Induces Receptor for Advanced Glycation End-Products (RAGE)-Dependent Sterile Inflammation in Amniotic Epithelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHelena Choltus,Régine Minet-Quinard,Corinne Belville,Julie Durif,Denis Gallot,Loic Blanchon,Vincent SapinPubMed 34361111
- Cell reports 35:1091022021RhoA/Cdc42 signaling drives cytoplasmic maturation but not endomitosis in megakaryocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTobias Heib,Heike M Hermanns,Georgi Manukjan,Maximilian Englert,Charly Kusch,Isabelle Carlotta Becker,Annika Gerber,Lou Martha Wackerbarth,Philipp Burkard,Thomas Dandekar,Johannes Balkenhol,Daniel Jahn,Sarah Beck,Mara Meub,Sebastian Dütting,Christian Stigloher,Markus Sauer,Deya Cherpokova,Harald Schulze,Cord Brakebusch,Bernhard Nieswandt,Zoltan Nagy,Irina PleinesPubMed 33979620
- The Journal of biological chemistry 296:1006802021Nonredundant roles of DIAPHs in primary ciliogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOliva Palander,Adam Lam,Richard F Collins,Theo J Moraes,William S TrimblePubMed 33872598
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:16516-165352020DIAPH1 regulates ciliogenesis and trafficking in primary cilia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOliva Palander,William S TrimblePubMed 33124112
- American journal of physiology. Renal physiology 315:F1601-F16122018Deletion of the formin Diaph1 protects from structural and functional abnormalities in the murine diabetic kidney.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichaele B Manigrasso et. al.PubMed 30132346
- The Journal of clinical investigation 128:4057-40732018The endothelial cell receptor stabilin-2 regulates VWF-FVIII complex half-life and immunogenicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura L Swystun et. al.PubMed 30124466
- Oncotarget 6:18577-892015Drosophila homologue of Diaphanous 1 (DIAPH1) controls the metastatic potential of colon cancer cells by regulating microtubule-dependent adhesion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan-Na Lin,Ridhirama Bhuwania,Kira Gromova,Antonio Virgilio Failla,Tobias Lange,Kristoffer Riecken,Stefan Linder,Matthias Kneussel,Jakob R Izbicki,Sabine WindhorstPubMed 26124177
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DIAPH1. The exact immunogen used to generate this antibody is proprietary information. Database link O60610
Reactivity data
Select an application| IHC-P | WB | ICC/IF | IHC-Fr | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Expected |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000.00000 - 1/15000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Gorilla, Orangutan | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Gorilla, Orangutan | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers (By similarity). Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization (By similarity). Required for cytokinesis, and transcriptional activation of the serum response factor (By similarity). DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity). Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity. Acts in a Rho-dependent manner to recruit PFY1 to the membrane (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells (PubMed:20937854, PubMed:21834987, PubMed:26912466). The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex (PubMed:20937854, PubMed:21834987). It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity (PubMed:20937854, PubMed:21834987). In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (PubMed:20937854, PubMed:21834987). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape (PubMed:20937854, PubMed:21834987). Plays a role in brain development (PubMed:24781755). Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity (By similarity).
Alternative names
DIAP1, DIAPH1, Protein diaphanous homolog 1, Diaphanous-related formin-1, DRF1
Recommended products
Rabbit Polyclonal DIAPH1 antibody. Suitable for IHC-P, WB, ICC/IF, IHC-Fr and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human DIAPH1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DIAPH1. The exact immunogen used to generate this antibody is proprietary information. Database link O60610
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DIAPH1 also known as Diaphanous-related formin 1 operates mainly in actin polymerization and is critical for filament formation. It weighs approximately 140 kDa. DIAPH1 expresses in a variety of tissues including heart liver and kidney making it a target of interest in multiple biological contexts. The protein plays a role in maintaining proper cytoskeletal dynamics by regulating the assembly of actin filaments.
- Biological function summary
DIAPH1 functions as part of the formin family which are important in cytoskeletal organization. It interacts with other proteins to initiate and elongate actin filaments often acting within larger protein complexes that control cellular morphology and movement. DIAPH1 contributes to muscle contraction cell division and cell migration highlighting its importance in cellular structure and dynamics.
- Pathways
DIAPH1 plays a significant role in the Rho GTPase signaling pathway. This pathway connects to cytoskeleton remodeling impacting cell shape and movement. DIAPH1 associates with proteins like RhoA which regulates its activity. Additionally DIAPH1 influences the Wnt signaling pathway known for its involvement in cell proliferation and differentiation.
- Associated diseases and disorders
DIAPH1 is connected to a form of sensorineural hearing loss and leukemia. Mutations in DIAPH1 can lead to autosomal dominant deafness as it affects the proper maintenance of actin structures in hair cells within the inner ear. The protein also interacts with genes linked to cancer development including those coding for p53 influencing tumorigenesis and cell cycle regulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Western blot - Anti-DIAPH1 antibody (ab11173)
All lanes: Western blot - Anti-DIAPH1 antibody (ab11173) at 0.66 µg/mL
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg
Lane 2: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Predicted band size: 141 kDa
Exposure time: 1s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIAPH1 antibody (ab11173)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DIAPH1 with ab11173 at 1/1000 (1µg/ml). Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIAPH1 antibody (ab11173)
IHC image of ab11173 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11173, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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