AB11173

Anti-DIAPH1 antibody

Name: Anti-DIAPH1 antibody (ab11173) | Abcam
Brand: Abcam Limited
SKU: AB11173
Price: 545 USD
Availability: InStock
Rating: 4 (3 reviews)

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(3 Reviews)

(14 Publications)

Rabbit Polyclonal DIAPH1 antibody. Suitable for IHC-P, WB, ICC/IF, IHC-Fr and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human DIAPH1.

View Alternative Names

DIAP1, DIAPH1, Protein diaphanous homolog 1, Diaphanous-related formin-1, DRF1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIAPH1 antibody (AB11173)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIAPH1 antibody (AB11173)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DIAPH1 with ab11173 at 1/1000 (1µg/ml). Detection : DAB.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIAPH1 antibody (AB11173)

IHC image of ab11173 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11173, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Supplier Data

Western blot - Anti-DIAPH1 antibody (AB11173)

All lanes:

Western blot - Anti-DIAPH1 antibody (ab11173) at 0.66 µg/mL

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 141 kDa

false

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-Fr, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human DIAPH1. The exact immunogen used to generate this antibody is proprietary information.

O60610

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/15000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibodies were affinity purified using the peptide immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DIAPH1 also known as Diaphanous-related formin 1 operates mainly in actin polymerization and is critical for filament formation. It weighs approximately 140 kDa. DIAPH1 expresses in a variety of tissues including heart liver and kidney making it a target of interest in multiple biological contexts. The protein plays a role in maintaining proper cytoskeletal dynamics by regulating the assembly of actin filaments.

Biological function summary

DIAPH1 functions as part of the formin family which are important in cytoskeletal organization. It interacts with other proteins to initiate and elongate actin filaments often acting within larger protein complexes that control cellular morphology and movement. DIAPH1 contributes to muscle contraction cell division and cell migration highlighting its importance in cellular structure and dynamics.

Pathways

DIAPH1 plays a significant role in the Rho GTPase signaling pathway. This pathway connects to cytoskeleton remodeling impacting cell shape and movement. DIAPH1 associates with proteins like RhoA which regulates its activity. Additionally DIAPH1 influences the Wnt signaling pathway known for its involvement in cell proliferation and differentiation.

DIAPH1 is connected to a form of sensorineural hearing loss and leukemia. Mutations in DIAPH1 can lead to autosomal dominant deafness as it affects the proper maintenance of actin structures in hair cells within the inner ear. The protein also interacts with genes linked to cancer development including those coding for p53 influencing tumorigenesis and cell cycle regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers (By similarity). Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization (By similarity). Required for cytokinesis, and transcriptional activation of the serum response factor (By similarity). DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity). Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity. Acts in a Rho-dependent manner to recruit PFY1 to the membrane (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells (PubMed : 20937854, PubMed : 21834987, PubMed : 26912466). The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex (PubMed : 20937854, PubMed : 21834987). It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity (PubMed : 20937854, PubMed : 21834987). In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (PubMed : 20937854, PubMed : 21834987). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape (PubMed : 20937854, PubMed : 21834987). Plays a role in brain development (PubMed : 24781755). Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity (By similarity).

See full target information DIAPH1

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 35:ar133 PubMed39196658

2024

mDia2 is an important mediator of MRTF-A-dependent regulation of breast cancer cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Ian Eder,Virginia Yu,Jacob Antonello,Fangyuan Chen,David Gau,Pooja Chawla,Marion Joy,Peter C Lucas,David Boone,Adrian V Lee,Partha Roy

Journal of molecular medicine (Berlin, Germany) 101:1015-1028 PubMed37462767

2023

Novel insights into the nervous system affected by prolonged hyperglycemia.

Applications

Unspecified application

Species

Unspecified reactive species

Kamila Zglejc-Waszak,Konark Mukherjee,Agnieszka Korytko,Bogdan Lewczuk,Andrzej Pomianowski,Joanna Wojtkiewicz,Marta Banach,Michał Załęcki,Natalia Nowicka,Julia Jarosławska,Bernard Kordas,Krzysztof Wąsowicz,Judyta K Juranek

Communications biology 6:280 PubMed36932214

2023

DIAPH1 mediates progression of atherosclerosis and regulates hepatic lipid metabolism in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Senatus,Lander Egaña-Gorroño,Raquel López-Díez,Sonia Bergaya,Juan Francisco Aranda,Jaume Amengual,Lakshmi Arivazhagan,Michaele B Manigrasso,Gautham Yepuri,Ramesh Nimma,Kaamashri N Mangar,Rollanda Bernadin,Boyan Zhou,Paul F Gugger,Huilin Li,Richard A Friedman,Neil D Theise,Alexander Shekhtman,Edward A Fisher,Ravichandran Ramasamy,Ann Marie Schmidt

Neuropathology : official journal of the Japanese Society of Neuropathology 43:84-94 PubMed35915909

2022

The receptor for advanced glycation end products and its ligands' expression in OVE26 diabetic sciatic nerve during the development of length-dependent neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Kamila Zglejc-Waszak,Ann Marie Schmidt,Judyta K Juranek

International journal of molecular sciences 22: PubMed34361111

2021

Cigarette Smoke Condensate Exposure Induces Receptor for Advanced Glycation End-Products (RAGE)-Dependent Sterile Inflammation in Amniotic Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Helena Choltus,Régine Minet-Quinard,Corinne Belville,Julie Durif,Denis Gallot,Loic Blanchon,Vincent Sapin

Cell reports 35:109102 PubMed33979620

2021

RhoA/Cdc42 signaling drives cytoplasmic maturation but not endomitosis in megakaryocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Tobias Heib,Heike M Hermanns,Georgi Manukjan,Maximilian Englert,Charly Kusch,Isabelle Carlotta Becker,Annika Gerber,Lou Martha Wackerbarth,Philipp Burkard,Thomas Dandekar,Johannes Balkenhol,Daniel Jahn,Sarah Beck,Mara Meub,Sebastian Dütting,Christian Stigloher,Markus Sauer,Deya Cherpokova,Harald Schulze,Cord Brakebusch,Bernhard Nieswandt,Zoltan Nagy,Irina Pleines

The Journal of biological chemistry 296:100680 PubMed33872598

2021

Nonredundant roles of DIAPHs in primary ciliogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Oliva Palander,Adam Lam,Richard F Collins,Theo J Moraes,William S Trimble

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:16516-16535 PubMed33124112

2020

DIAPH1 regulates ciliogenesis and trafficking in primary cilia.

Applications

Unspecified application

Species

Unspecified reactive species

Oliva Palander,William S Trimble

American journal of physiology. Renal physiology 315:F1601-F1612 PubMed30132346

2018

Deletion of the formin Diaph1 protects from structural and functional abnormalities in the murine diabetic kidney.

Applications

Unspecified application

Species

Unspecified reactive species

Michaele B Manigrasso,Richard A Friedman,Ravichandran Ramasamy,Vivette D'Agati,Ann Marie Schmidt

The Journal of clinical investigation 128:4057-4073 PubMed30124466

2018

The endothelial cell receptor stabilin-2 regulates VWF-FVIII complex half-life and immunogenicity.

Applications

Unspecified application

Species

Unspecified reactive species

Laura L Swystun,Jesse D Lai,Colleen Notley,Ilinca Georgescu,A Simonne Paine,Jeff Mewburn,Kate Nesbitt,Kai Schledzewski,Cyrill Géraud,Julia Kzhyshkowska,Sergij Goerdt,Wilma Hopman,Robert R Montgomery,Paula D James,David Lillicrap

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DIAPH1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIAPH1 antibody (AB11173)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIAPH1 antibody (AB11173)

Western blot - Anti-DIAPH1 antibody (AB11173)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise