AB181165

Anti-DIAPH2/DIA antibody [EPR13158]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal DIAPH2/DIA antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DIA, DIAPH2, Protein diaphanous homolog 2, Diaphanous-related formin-2, DRF2

1 Images

Supplier Data

Western blot - Anti-DIAPH2/DIA antibody [EPR13158] (AB181165)

All lanes:

Western blot - Anti-DIAPH2/DIA antibody [EPR13158] (ab181165) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HCT-116 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 126 kDa

Observed band size: 130 kDa

false

Carrier free

Anti-DIAPH2/DIA antibody [EPR13158] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13158

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DIAPH2 also known as DIA or Diaphanous-related formin-2 is a protein that functions as a formin homology protein involved in actin polymerization. It plays an important role in the dynamic reorganization of the cytoskeleton. DIAPH2 has a molecular weight of approximately 123 kDa. Scientists have identified its expression in various tissues though it is observed significantly in the brain testis and kidney. It participates actively in bridging components of the cytoskeleton and membrane signaling complexes.

Biological function summary

DIAPH2 influences cellular processes by regulating actin filament elongation and stabilization. This protein associates with the Rho family of GTPases forming a critical complex that drives localized actin assembly. This actin polymerization is necessary for processes like cell division cell migration and maintenance of cell shape. Additionally DIAPH2 plays roles in vesicle trafficking and the formation of cellular structures like filopodia and lamellipodia linking membrane processes with cytoskeletal elements.

Pathways

DIAPH2 integrates into significant pathways such as the Rho-GTPase pathway where it acts as an effector that transduces signals from upstream activators like Rho and Rac proteins. These pathways regulate the assembly and disassembly of actin filaments critical for various cell functions. Through its interactions with other proteins such as mDia1 and ROCK DIAPH2 influences the actin cytoskeleton impacting pathways involved in cell motility and adhesion.

Researchers have linked mutations in the DIAPH2 gene with disorders like premature ovarian failure (POF) and certain types of cancer. In POF DIAPH2 disrupts normal ovarian function impacting fertility. In cancer aberrant regulation of DIAPH2 can lead to improper cell division and tumor growth. Studies have also suggested interactions with proteins such as BRCA1 indicating a potential role in pathways involved in maintaining genomic stability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.

See full target information DIAPH2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadw4609 PubMed40971433

2025

Nipah virus matrix protein uses cortical actin to stabilize the virus assembly sites and promote budding.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Wang,Vicky Kliemke,Mengyu Zhang,Jinxin Liu,Giuliana Leonarda Matta,Qian Wang,Yuhang Luo,GuanQun Liu,Qian Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DIAPH2/DIA antibody [EPR13158]

Western blot - Anti-DIAPH2/DIA antibody [EPR13158] (AB181165)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise