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AB250391

Anti-DIAPH2/DIA antibody [EPR13158] - BSA and Azide free

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Rabbit Recombinant Monoclonal DIAPH2/DIA antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

DIA, DIAPH2, Protein diaphanous homolog 2, Diaphanous-related formin-2, DRF2

1 Images
Western blot - Anti-DIAPH2/DIA antibody [EPR13158] - BSA and Azide free (AB250391)
  • WB

Supplier Data

Western blot - Anti-DIAPH2/DIA antibody [EPR13158] - BSA and Azide free (AB250391)

This data was developed using ab181165, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DIAPH2/DIA antibody [EPR13158] (<a href='/en-us/products/primary-antibodies/diaph2-dia-antibody-epr13158-ab181165'>ab181165</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HCT-116 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 126 kDa

Observed band size: 130 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13158

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250391 is the carrier-free version of ab181165.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DIAPH2 also known as DIA or Diaphanous-related formin-2 is a protein that functions as a formin homology protein involved in actin polymerization. It plays an important role in the dynamic reorganization of the cytoskeleton. DIAPH2 has a molecular weight of approximately 123 kDa. Scientists have identified its expression in various tissues though it is observed significantly in the brain testis and kidney. It participates actively in bridging components of the cytoskeleton and membrane signaling complexes.
Biological function summary

DIAPH2 influences cellular processes by regulating actin filament elongation and stabilization. This protein associates with the Rho family of GTPases forming a critical complex that drives localized actin assembly. This actin polymerization is necessary for processes like cell division cell migration and maintenance of cell shape. Additionally DIAPH2 plays roles in vesicle trafficking and the formation of cellular structures like filopodia and lamellipodia linking membrane processes with cytoskeletal elements.

Pathways

DIAPH2 integrates into significant pathways such as the Rho-GTPase pathway where it acts as an effector that transduces signals from upstream activators like Rho and Rac proteins. These pathways regulate the assembly and disassembly of actin filaments critical for various cell functions. Through its interactions with other proteins such as mDia1 and ROCK DIAPH2 influences the actin cytoskeleton impacting pathways involved in cell motility and adhesion.

Researchers have linked mutations in the DIAPH2 gene with disorders like premature ovarian failure (POF) and certain types of cancer. In POF DIAPH2 disrupts normal ovarian function impacting fertility. In cancer aberrant regulation of DIAPH2 can lead to improper cell division and tumor growth. Studies have also suggested interactions with proteins such as BRCA1 indicating a potential role in pathways involved in maintaining genomic stability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.
See full target information DIAPH2
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Product promise

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For full details, please see our Terms & Conditions

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