Mouse Polyclonal DIAPH3 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DIAPH3.
Images
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human DIAPH3. Database link Q9NSV4
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor. Binds to GTP-bound form of Rho and to profilin: acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity.
Alternative names
DIAP3, DIAPH3, Protein diaphanous homolog 3, Diaphanous-related formin-3, MDia2, DRF3
Recommended products
Mouse Polyclonal DIAPH3 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DIAPH3.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human DIAPH3. Database link Q9NSV4
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DIAPH3 also known as diaphanous-related formin 3 is a protein with an approximate mass of 130 kDa. It plays an important role in the regulation of the actin cytoskeleton essential for the dynamic reorganization of actin filaments. DIAPH3 primarily functions by mediating actin nucleation and elongation facilitating cellular processes such as shape changes adhesion and division. The protein is expressed in various tissues with notable expression in the brain testis and pancreas.
- Biological function summary
DIAPH3 is a part of the formin family and acts by promoting the polymerization of actin filaments. It forms a complex with profilin and other actin-binding proteins maintaining the balance of cellular architecture. DIAPH3 contributes to the maintenance of normal cellular structure and dynamics participating in cell migration and morphogenesis. Its interaction with GTPases like RhoA regulates the initiation and stabilization of actin filaments directly impacting cell mechanics.
- Pathways
DIAPH3 engages in the Rho GTPase signaling pathway impacting actin dynamics and playing roles in cytoskeletal remodelling. It also participates in the mTOR signaling pathway influencing cell growth and metabolism. Within these pathways DIAPH3 works closely with proteins such as mTOR contributing to cellular responses like growth proliferation and survival. These pathways highlight DIAPH3’s involvement in coordinating cellular structural integrity and function.
- Associated diseases and disorders
Research has implicated DIAPH3 in sensory hearing loss and cancer progression. In hearing loss mutations in DIAPH3 disrupt the organization of actin filaments critical for auditory function. In the context of cancer abnormal DIAPH3 expression relates to increased cellular motility and invasion facilitating metastasis. In these conditions DIAPH3 interacts with proteins such as E-cadherin affecting cellular adhesion and communication and emphasizing its significance in disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-DIAPH3 antibody (ab172701)
All lanes: Western blot - Anti-DIAPH3 antibody (ab172701) at 1 µg/mL
Lane 1: DIAPH3 transfected 293T cell lysate at 15 µL
Lane 2: Non-transfected 293T cell lysate at 15 µL
SecondaryAll lanes: Goat Anti-mouse HRP Conjugate at 1/2500 dilution
Predicted band size: 137 kDa
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