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AB189373

Anti-DIAPH3 antibody

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(3 Publications)

Rabbit Polyclonal DIAPH3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DIAPH3 aa 700-1150.

View Alternative Names

DIAP3, DIAPH3, Protein diaphanous homolog 3, Diaphanous-related formin-3, MDia2, DRF3

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIAPH3 antibody (AB189373)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIAPH3 antibody (AB189373)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human adrenal tissue, labeling DIAPH3 using ab189373 at 10 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DIAPH3 aa 700-1150. The exact immunogen used to generate this antibody is proprietary information.

Q9NSV4

Specificity

ab189373 is reactive against recombinant protein, detects immunogen.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.79% Tris glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DIAPH3 also known as diaphanous-related formin 3 is a protein with an approximate mass of 130 kDa. It plays an important role in the regulation of the actin cytoskeleton essential for the dynamic reorganization of actin filaments. DIAPH3 primarily functions by mediating actin nucleation and elongation facilitating cellular processes such as shape changes adhesion and division. The protein is expressed in various tissues with notable expression in the brain testis and pancreas.
Biological function summary

DIAPH3 is a part of the formin family and acts by promoting the polymerization of actin filaments. It forms a complex with profilin and other actin-binding proteins maintaining the balance of cellular architecture. DIAPH3 contributes to the maintenance of normal cellular structure and dynamics participating in cell migration and morphogenesis. Its interaction with GTPases like RhoA regulates the initiation and stabilization of actin filaments directly impacting cell mechanics.

Pathways

DIAPH3 engages in the Rho GTPase signaling pathway impacting actin dynamics and playing roles in cytoskeletal remodelling. It also participates in the mTOR signaling pathway influencing cell growth and metabolism. Within these pathways DIAPH3 works closely with proteins such as mTOR contributing to cellular responses like growth proliferation and survival. These pathways highlight DIAPH3’s involvement in coordinating cellular structural integrity and function.

Research has implicated DIAPH3 in sensory hearing loss and cancer progression. In hearing loss mutations in DIAPH3 disrupt the organization of actin filaments critical for auditory function. In the context of cancer abnormal DIAPH3 expression relates to increased cellular motility and invasion facilitating metastasis. In these conditions DIAPH3 interacts with proteins such as E-cadherin affecting cellular adhesion and communication and emphasizing its significance in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor. Binds to GTP-bound form of Rho and to profilin : acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity.
See full target information DIAPH3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular biosciences 9:910950 PubMed36589226

2022

Diaphanous-related formin subfamily: Novel prognostic biomarkers and tumor microenvironment regulators for pancreatic adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bixi Zhang,Qing Hu,Yanchun Li,Canxia Xu,Xiaoran Xie,Peng Liu,Meihua Xu,Siming Gong,Hao Wu

Discover. Oncology 12:20 PubMed35201449

2021

Diaphanous related formin 3 knockdown suppresses cell proliferation and metastasis of osteosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zehua Zhang,Fei Dai,Fei Luo,Wenjie Wu,Shuai Zhang,Rui Zhou,Jianzhong Xu,Qiang Zhou,Lei Song

Tissue engineering. Part A 26:1030-1041 PubMed32368948

2020

Engineering a Corneal Stromal Equivalent Using a Novel Multilayered Fabrication Assembly Technique.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Fernández-Pérez,Peter W Madden,Mark Ahearne
View all publications
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