AB245659

Anti-DIAPH3 antibody

Name: Anti-DIAPH3 antibody (ab245659) | Abcam
Brand: Abcam Limited
SKU: AB245659

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal DIAPH3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human DIAPH3 aa 1-50.

View Alternative Names

DIAP3, DIAPH3, Protein diaphanous homolog 3, Diaphanous-related formin-3, MDia2, DRF3

2 Images

Supplier Data

Immunoprecipitation - Anti-DIAPH3 antibody (AB245659)

DIAPH3 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

ab245659 used for IP at 6 μg per reaction. For WB 1 μg/ml.

Lane 1 : ab245659 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.

Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-DIAPH3 antibody (ab245659)

Predicted band size: 137 kDa

false

Supplier Data

Western blot - Anti-DIAPH3 antibody (AB245659)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-DIAPH3 antibody (ab245659) at 0.1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 137 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human DIAPH3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9NSV4

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab245659 was affinity purified using an epitope specific to DIAPH3 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DIAPH3 also known as diaphanous-related formin 3 is a protein with an approximate mass of 130 kDa. It plays an important role in the regulation of the actin cytoskeleton essential for the dynamic reorganization of actin filaments. DIAPH3 primarily functions by mediating actin nucleation and elongation facilitating cellular processes such as shape changes adhesion and division. The protein is expressed in various tissues with notable expression in the brain testis and pancreas.

Biological function summary

DIAPH3 is a part of the formin family and acts by promoting the polymerization of actin filaments. It forms a complex with profilin and other actin-binding proteins maintaining the balance of cellular architecture. DIAPH3 contributes to the maintenance of normal cellular structure and dynamics participating in cell migration and morphogenesis. Its interaction with GTPases like RhoA regulates the initiation and stabilization of actin filaments directly impacting cell mechanics.

Pathways

DIAPH3 engages in the Rho GTPase signaling pathway impacting actin dynamics and playing roles in cytoskeletal remodelling. It also participates in the mTOR signaling pathway influencing cell growth and metabolism. Within these pathways DIAPH3 works closely with proteins such as mTOR contributing to cellular responses like growth proliferation and survival. These pathways highlight DIAPH3’s involvement in coordinating cellular structural integrity and function.

Research has implicated DIAPH3 in sensory hearing loss and cancer progression. In hearing loss mutations in DIAPH3 disrupt the organization of actin filaments critical for auditory function. In the context of cancer abnormal DIAPH3 expression relates to increased cellular motility and invasion facilitating metastasis. In these conditions DIAPH3 interacts with proteins such as E-cadherin affecting cellular adhesion and communication and emphasizing its significance in disease mechanisms.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor. Binds to GTP-bound form of Rho and to profilin : acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity.

See full target information DIAPH3

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Endokrynologia Polska 75:501-509 PubMed39376176

2024

The functional DIAPH3-FOXM1 interaction modulates the aggressive transformation of anaplastic thyroid carcinoma cells and Wnt/β-catenin signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Shi,Hao Fang,Kai Fu,Zhen Zhao,Fei Yang,Yan Liu

Molecular medicine reports 25: PubMed34878155

2021

IRF2 contributes to myocardial infarction via regulation of GSDMD induced pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yongxing Li,Yan Wang,Hua Guo,Qinghua Wu,Yamin Hu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DIAPH3 antibody

Immunoprecipitation - Anti-DIAPH3 antibody (AB245659)

Western blot - Anti-DIAPH3 antibody (AB245659)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise