AB227518

Anti-Dicer antibody

Name: Anti-Dicer antibody (ab227518) | Abcam
Brand: Abcam Limited
SKU: AB227518
Rating: 4 (1 reviews)

(1 Review)

(14 Publications)

Rabbit Polyclonal DICER antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DICER1.

View Alternative Names

DICER, HERNA, KIAA0928, DICER1, Endoribonuclease Dicer, Helicase with RNase motif, Helicase MOI

2 Images

Supplier Data

Western blot - Anti-Dicer antibody (AB227518)

5% SDS-PAGE

All lanes:

Western blot - Anti-Dicer antibody (ab227518) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

Lanes 2 - 3:

Dicer shRNA transfected MCF7 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 218 kDa

true

Supplier Data

Western blot - Anti-Dicer antibody (AB227518)

5% SDS-PAGE

All lanes:

Western blot - Anti-Dicer antibody (ab227518) at 1/500 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg

Lane 2:

C8D30 whole cell lysate at 30 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Lane 4:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg

Lane 5:

C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 218 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human DICER1. The exact immunogen used to generate this antibody is proprietary information.

Q9UPY3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.

See full target information DICER1

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40649978

2025

Exploring the Role of Peripheral Macrophages in Glioma Progression: The Metabolic Significance of Cyclooxygenase-2 (COX-2).

Applications

Unspecified application

Species

Unspecified reactive species

Jens Pietzsch,Magali Toussaint,Cornelius Kurt Donat,Alina Doctor,Sebastian Meister,Johanna Wodtke,Markus Laube,Frank Hofheinz,Jan Rix,Winnie Deuther-Conrad,Cathleen Haase-Kohn

Science advances 11:eadt3751 PubMed40614179

2025

Dicer is cleaved by the Leader protease encoded by foot-and-mouth disease virus to promote infection in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel Rodríguez-Pulido,Miguel Ángel Sanz,Lucía Camacho,Ricardo Ramos,Margarita Sáiz

MedComm 5:e475 PubMed38463393

2024

AMPK-upregulated microRNA-708 plays as a suppressor of cellular senescence and aging via downregulating disabled-2 and mTORC1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Zhang,Hui Gong,Tingting Zhao,Weitong Xu,Honghan Chen,Tiepeng Li,Yu Yang,Ming Yang,Ning Huang,Chuhui Gong,Fangfang Wang,Cuiying Zhang,Jin Liu,Hengyi Xiao

Cell reports. Medicine 4:101209 PubMed37757825

2023

Hyperglycemia-regulated tRNA-derived fragment tRF-3001a propels neurovascular dysfunction in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Ya Zhu,Wen Yao,Xi-Sen Ni,Mu-Di Yao,Wen Bai,Tian-Jing Yang,Zi-Ran Zhang,Xiu-Miao Li,Qin Jiang,Biao Yan

International journal of molecular sciences 23: PubMed36077457

2022

Tankyrase Inhibition Attenuates Cardiac Dilatation and Dysfunction in Ischemic Heart Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Wang,Heli Segersvärd,Juuso Siren,Sanni Perttunen,Katariina Immonen,Riikka Kosonen,Yu-Chia Chen,Johanna Tolva,Mirjami Laivuori,Mikko I Mäyränpää,Petri T Kovanen,Juha Sinisalo,Mika Laine,Ilkka Tikkanen,Päivi Lakkisto

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22412 PubMed35713587

2022

The hypoxia-induced changes in miRNA-mRNA in RNA-induced silencing complexes and HIF-2 induced miRNAs in human endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Adrianna Moszyńska,Maciej Jaśkiewicz,Marcin Serocki,Aleksandra Cabaj,David K Crossman,Sylwia Bartoszewska,Magdalena Gebert,Michał Dąbrowski,James F Collawn,Rafal Bartoszewski

Nature communications 12:3497 PubMed34108453

2021

Site-specific ubiquitylation acts as a regulator of linker histone H1.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Höllmüller,Simon Geigges,Marie L Niedermeier,Kai-Michael Kammer,Simon M Kienle,Daniel Rösner,Martin Scheffner,Andreas Marx,Florian Stengel

Aging 13:10275-10288 PubMed33819188

2021

CircRNA hsa_circ_0006215 promotes osteogenic differentiation of BMSCs and enhances osteogenesis-angiogenesis coupling by competitively binding to miR-942-5p and regulating RUNX2 and VEGF.

Applications

Unspecified application

Species

Unspecified reactive species

Houlin Ji,Xu Cui,Yang Yang,Xiaoxiao Zhou

International journal of molecular medicine 47: PubMed33649815

2021

circNRIP1 facilitates keloid progression via FXR1‑mediated upregulation of miR‑503‑3p and miR‑503‑5p.

Applications

Unspecified application

Species

Unspecified reactive species

Baolin Wang,Hang Yin,Hongmei Zhang,Tiantian Wang

Epigenetics 16:1317-1334 PubMed33356812

2020

Upregulation of polycistronic microRNA-143 and microRNA-145 in colonocytes suppresses colitis and inflammation-associated colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Urszula Dougherty,Reba Mustafi,Hongyan Zhu,Xiaorong Zhu,Dilip Deb,Stephen C Meredith,Fatma Ayaloglu-Butun,Michelle Fletcher,Arantxa Sanchez,Joel Pekow,Zifeng Deng,Nader Amini,Vani J Konda,Vijaya L Rao,Atsushi Sakuraba,Akushika Kwesi,Sonia S Kupfer,Alessandro Fichera,Loren Joseph,John Hart,Fang He,Tong-Chuan He,Diana West-Szymanski,Yan Chun Li,Marc Bissonnette

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dicer antibody

Western blot - Anti-Dicer antibody (AB227518)

Western blot - Anti-Dicer antibody (AB227518)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (14)

Product promise