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AB264250

Anti-Dicer antibody

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(1 Publication)

Rabbit Polyclonal DICER antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DICER1 aa 1150-1250.

View Alternative Names

DICER, HERNA, KIAA0928, DICER1, Endoribonuclease Dicer, Helicase with RNase motif, Helicase MOI

2 Images
Immunoprecipitation - Anti-Dicer antibody (AB264250)
  • IP

Characteriser

Immunoprecipitation - Anti-Dicer antibody (AB264250)

Dicer was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264250 at 10 μg per reaction. Western blot was performed on the immunoprecipitates using ab264250 at 1 μg/ml.

Lane 1 : ab264250 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Dicer antibody (ab264250)

Predicted band size: 218 kDa

false

Western blot - Anti-Dicer antibody (AB264250)
  • WB

Characteriser

Western blot - Anti-Dicer antibody (AB264250)

4-8% SDS-PAGE

All lanes:

Western blot - Anti-Dicer antibody (ab264250) at 0.1 µg/mL

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 2:

HEK-293T whole cell lysate at 15 µg

Lane 3:

HEK-293T whole cell lysate at 5 µg

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Predicted band size: 218 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human DICER1 aa 1150-1250. The exact immunogen used to generate this antibody is proprietary information.

Q9UPY3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5-15 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
See full target information DICER1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Viruses 14: PubMed35893693

2022

Inhibition of Venezuelan Equine Encephalitis Virus Using Small Interfering RNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Amrita Haikerwal,Michael D Barrera,Nishank Bhalla,Weidong Zhou,Niloufar Boghdeh,Carol Anderson,Farhang Alem,Aarthi Narayanan
View all publications
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Product promise

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