Rabbit Recombinant Monoclonal DICER antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Cat, Dog, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human DICER1.
Images
![Western blot - Anti-Dicer antibody [HL1232] (AB308349), expandable thumbnail](https://content.abcam.com/products/images/dicer-antibody-hl1232-ab308349--western-blot-img441340.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Dicer antibody [HL1232] (AB308349)")
![Western blot - Anti-Dicer antibody [HL1232] (AB308349), expandable thumbnail](https://content.abcam.com/products/images/dicer-antibody-hl1232-ab308349--western-blot-img441339.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Dicer antibody [HL1232] (AB308349)")
![Western blot - Anti-Dicer antibody [HL1232] (AB308349), expandable thumbnail](https://content.abcam.com/products/images/dicer-antibody-hl1232-ab308349--western-blot-img407974.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Dicer antibody [HL1232] (AB308349)")
![Immunocytochemistry/ Immunofluorescence - Anti-Dicer antibody [HL1232] (AB308349), expandable thumbnail](https://content.abcam.com/products/images/dicer-antibody-hl1232-ab308349--immunocytochemistry-immunofluorescence-img407977.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Dicer antibody [HL1232] (AB308349)")
![Western blot - Anti-Dicer antibody [HL1232] (AB308349), expandable thumbnail](https://content.abcam.com/products/images/dicer-antibody-hl1232-ab308349--western-blot-img407976.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Dicer antibody [HL1232] (AB308349)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human DICER1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UPY3
Reactivity data
Select an application| ICC/IF | WB | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Expected | Tested |
| Cat | Expected | Tested |
| Dog | Expected | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cat, Dog, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Cat | Dilution info 1/500 - 1/3000 | Notes - |
Species Dog | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Target data
Function
Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
Alternative names
DICER, HERNA, KIAA0928, DICER1, Endoribonuclease Dicer, Helicase with RNase motif, Helicase MOI
Recommended products
Rabbit Recombinant Monoclonal DICER antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Cat, Dog, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human DICER1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human DICER1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UPY3
- Carrier free
- Yes
- Clone number
- HL1232
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Dicer antibody [HL1232] (ab308349), expandable thumbnail](https://content.abcam.com/products/images/dicer-antibody-hl1232-ab308349--western-blot-img441340.jpg "Western blot - Anti-Dicer antibody [HL1232] (ab308349)")
Western blot - Anti-Dicer antibody [HL1232] (ab308349)
Samples were seperated by 5% SDS-PAGE.
All lanes: Western blot - Anti-Dicer antibody [HL1232] (ab308349) at 1/1000 dilution
Lane 1: 293T whole cell extracts at 30 µg
Lane 2: A431 whole cell extracts at 30 µg
Lane 3: HeLa whole cell extracts at 30 µg
Lane 4: HepG2 whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 218 kDa
![Western blot - Anti-Dicer antibody [HL1232] (ab308349), expandable thumbnail](https://content.abcam.com/products/images/dicer-antibody-hl1232-ab308349--western-blot-img441339.jpg "Western blot - Anti-Dicer antibody [HL1232] (ab308349)")
Western blot - Anti-Dicer antibody [HL1232] (ab308349)
Samples were seperated by 5% SDS-PAGE.
All lanes: Western blot - Anti-Dicer antibody [HL1232] (ab308349) at 1/1000 dilution
Lane 1: Non-transfected (–) MCF-7 whole cell extracts at 30 µg
Lane 2: Transfected (+) MCF-7 whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 218 kDa
![Western blot - Anti-Dicer antibody [HL1232] (ab308349), expandable thumbnail](https://content.abcam.com/products/images/dicer-antibody-hl1232-ab308349--western-blot-img407974.jpg "Western blot - Anti-Dicer antibody [HL1232] (ab308349)")
Western blot - Anti-Dicer antibody [HL1232] (ab308349)
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (ab308349) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-Dicer antibody [HL1232] (ab308349) at 1/1000 dilution
Lane 1: MDCK cell lysate at 30 µg
Lane 2: PG-4 cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 218.7 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Dicer antibody [HL1232] (ab308349), expandable thumbnail](https://content.abcam.com/products/images/dicer-antibody-hl1232-ab308349--immunocytochemistry-immunofluorescence-img407977.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Dicer antibody [HL1232] (ab308349)")
Immunocytochemistry/ Immunofluorescence - Anti-Dicer antibody [HL1232] (ab308349)
For immunofluorescence analysis, HeLa cells were fixed in 4% paraformaldehyde at room temperature for 15 min and permeabilized for detection of Dicer protein using ab308349 at 1/500 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI. Alpha Tubulin stained by alpha Tubulin antibody (red) diluted at 1/1000. Scale bar= 10μm.
![Western blot - Anti-Dicer antibody [HL1232] (ab308349), expandable thumbnail](https://content.abcam.com/products/images/dicer-antibody-hl1232-ab308349--western-blot-img407976.jpg "Western blot - Anti-Dicer antibody [HL1232] (ab308349)")
Western blot - Anti-Dicer antibody [HL1232] (ab308349)
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (ab308349) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
All lanes: Western blot - Anti-Dicer antibody [HL1232] (ab308349) at 1/1000 dilution
Lane 1: PC-12 cell lysate at 30 µg
Lane 2: Rat2 cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 218.7 kDa
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