AB167444

Anti-Dicer antibody [N167/7]

Name: Anti-Dicer antibody [N167/7] (ab167444) | Abcam
Brand: Abcam Limited
SKU: AB167444

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(3 Publications)

Mouse Monoclonal DICER antibody. Suitable for WB, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Protein within Mouse Dicer1 aa 1600 to C-terminus.

View Alternative Names

DICER, HERNA, KIAA0928, DICER1, Endoribonuclease Dicer, Helicase with RNase motif, Helicase MOI

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Dicer antibody [N167/7] (AB167444)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Dicer antibody [N167/7] (AB167444)

4% formaldehyde-fixed SK-N-BE cells labeling Dicer using ab167444 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/100 dilution incubated for 1 hour at room temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Dicer antibody [N167/7] (AB167444)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed Neuroblastoma cells (SH-SY5Y) labeling Dicer with ab167444 at 1/50 dilution. Secondary antibody : AlexaFluor® 488 at 1/1000 dilution. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Dicer Antibody (D) Composite.

Supplier Data

Western blot - Anti-Dicer antibody [N167/7] (AB167444)

Color Development : TMB solution for 10 min at RT.

Lane 1:

Western blot - Anti-Dicer antibody [N167/7] (ab167444)

Lane 2:

Western blot - Anti-Dicer antibody [N167/7] (ab167444) at 1/1000 dilution

Lane 1:

Molecular weight marker

Lane 2:

Rat Brain Membrane lysate at 10 µg

Secondary

All lanes:

Goat Anti-Mouse IgG at 1/200 dilution

Predicted band size: 218 kDa

Observed band size: 215 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N167/7

Isotype

IgG1

Carrier free

Reacts with

Rat, Human, Mouse

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Protein within Mouse Dicer1 aa 1600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8R418

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

The clone number has been updated from S167-7 to N167/7, both clone numbers name the same antibody clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.

See full target information DICER1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Canadian journal of gastroenterology & hepatology 2022:9202531 PubMed39296516

2022

Pumilio RNA-Binding Family Member 1 Plays a Promoting Role on Pancreatic Cancer Angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Haisu Dai,Yan Jiang,Zhipeng Liu,Xingxing Su,Yishi Yang,Zhiyu Chen

Nature neuroscience 23:167-171 PubMed31873194

2019

Microglial microRNAs mediate sex-specific responses to tau pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Lay Kodama,Elmer Guzman,Jon I Etchegaray,Yaqiao Li,Faten A Sayed,Lu Zhou,Yungui Zhou,Lihong Zhan,David Le,Joe C Udeochu,Claire D Clelland,Zuolin Cheng,Guoqiang Yu,Qingyun Li,Kenneth S Kosik,Li Gan

Addiction biology 24:498-508 PubMed29516602

2018

Ago2 and Dicer1 are involved in METH-induced locomotor sensitization in mice via biogenesis of miRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Liu,Li Zhu,Tong Ni,Fang-Lin Guan,Yan-Jiong Chen,Dong-Liang Ma,Eyleen L K Goh,Teng Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dicer antibody [N167/7]

Immunocytochemistry/ Immunofluorescence - Anti-Dicer antibody [N167/7] (AB167444)

Immunocytochemistry/ Immunofluorescence - Anti-Dicer antibody [N167/7] (AB167444)

Western blot - Anti-Dicer antibody [N167/7] (AB167444)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Product protocols

Target data

Publications (3)

Product promise