AB327103

Anti-DICER1 Antibody [CL0378]

Name: Anti-DICER1 Antibody [CL0378] (ab327103) | Abcam
Brand: Abcam Limited
SKU: AB327103
Price: 580 USD
Availability: InStock

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Mouse Monoclonal DICER antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DICER1.

View Alternative Names

DICER, HERNA, KIAA0928, DICER1, Endoribonuclease Dicer, Helicase with RNase motif, Helicase MOI

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DICER1 Antibody [CL0378] (AB327103)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DICER1 Antibody [CL0378] (AB327103)

Immunohistochemical staining of human cerebral cortex using ab327103 at 1 : 200–1 : 500 dilution, showing moderate cytoplasmic positivity in neurons.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DICER1 Antibody [CL0378] (AB327103)

Immunohistochemical staining of human breast cancer using ab327103 at 1 : 200–1 : 500 dilution, showing moderate cytoplasmic positivity in tumor cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DICER1 Antibody [CL0378] (AB327103)

Immunohistochemical staining of human endometrium using ab327103 at 1 : 200–1 : 500 dilution, showing moderate cytoplasmic positivity in glandular cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DICER1 Antibody [CL0378] (AB327103)

Immunohistochemical staining of human colorectal cancer using ab327103 at 1 : 200–1 : 500 dilution, showing moderate cytoplasmic positivity in tumor cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DICER1 Antibody [CL0378] (AB327103)

Immunohistochemical staining of human testis using ab327103 at 1 : 200–1 : 500 dilution, showing moderate to strong cytoplasmic positivity in a subset of cells in seminiferous ducts.

Supplier Data

Western blot - Anti-DICER1 Antibody [CL0378] (AB327103)

Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using ab327103 at 1 µg/ml. Remaining relative intensity is presented. Loading control : Anti-GAPDH.

All lanes:

Western blot - Anti-DICER1 Antibody [CL0378] (ab327103)

Lane 2:

RT-4 cells transfected with control siRNA

Lane 3:

RT-4 cells transfected with target specific siRNA probe #1

Lane 4:

RT-4 cells transfected with target specific siRNA probe #2

Predicted band size: 218.68 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0378

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DICER1.

Q9UPY3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.

See full target information DICER1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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