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AB288749

Anti-Digoxigenin antibody [DIG44]

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Llama Recombinant Monoclonal Digoxigenin antibody. Suitable for SDS-PAGE, ELISA, WB and reacts with Digitalis samples. Immunogen corresponding to Chemical / Small Molecule corresponding to Digoxigenin.

View Alternative Names

1672-46-4, BRN 0096479, DIG, EINECS 216-806-2, HSDB 7108, Lanadigenin, Lanadigigenin, ST056392

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ELISA - Anti-Digoxigenin antibody [DIG44] (AB288749)
  • ELISA

Supplier Data

ELISA - Anti-Digoxigenin antibody [DIG44] (AB288749)

ELISA Results of purified recombinant Anti-DIG Single Domain Antibody reactive against BSA-conjugated DIG. The working dilution for clone DIG44 is 1 : 1000. Each well was coated in duplicate with 1.0μg BSA-conjugated DIG (Red) and BSA only (Green). The starting dilution of antibody was 45μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Sodium Carbonate blocking buffer, Mouse Anti-6X HIS Epitope Tag HRP conjugated Antibody and TMB substrate.

SDS-PAGE - Anti-Digoxigenin antibody [DIG44] (AB288749)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Anti-Digoxigenin antibody [DIG44] (AB288749)

SDS-PAGE Results of Recombinant Anti-DIG (VhH) Single Domain Antibody. Lane 1 : Anti-DIG-VhH (Clone DIG44) Reduced (1μg). Lane 2 : Prestained Molecular Weight Marker. Lane 3 : Anti-DIG-VhH (Clone DIG44) Non-Reduced (1μg). 4-20% Gel Coomassie Blue stained. Predicted MW : ~15kDa. Observed MW : ~18kDa Reduced, ~16kDa Non-Reduced.

Key facts

Host species

Llama

Clonality

Monoclonal

Clone number

DIG44

Isotype

VHH

Carrier free

No

Reacts with

Digitalis

Applications

ELISA, SDS-PAGE, WB

applications

Specificity

<p>Expect reactivity against DIG-labeled probes and proteins.</p>

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Complementary Products

Primary Antibodies

AB420

Anti-Digoxigenin antibody [21H8]

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Primary Antibodies

AB288734

Anti-Digoxigenin antibody [DIG45]

Recombinant

ELISA - Anti-Digoxigenin antibody [DIG45] (AB288734)

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Sample Prep & Detection Kits

AB102890

HRP Conjugation Kit - Lightning-Link®

Conjugation - HRP Conjugation Kit - Lightning-Link® (AB102890)

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Primary Antibodies

AB76907

Anti-Digoxigenin antibody

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "SDSPAGE" : {"fullname" : "SDS-PAGE", "shortname":"SDS-PAGE"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Digitalis": { "SDSPAGE-species-checked": "testedAndGuaranteed", "SDSPAGE-species-dilution-info": "", "SDSPAGE-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/1000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Anti-DIG (VHH) Antibody is a recombinant antibody. The clone was isolated from a library prepared from a hyper-immunized llama host and purified by affinity chromatography from bacterial culture.
Storage buffer
pH: 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Digoxigenin
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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