Anti-Digoxigenin antibody [DIG45]

• ELISA

Supplier Data

ELISA - Anti-Digoxigenin antibody [DIG45] (AB288734)

ELISA Results of purified recombinant Anti-DIG Single Domain Antibody reactive against BSA-conjugated DIG. The working dilution for clone DIG45 is 1 : 2000. Each well was coated in duplicate with 1.0μg of BSA conjugated DIG (Blue) and BSA only (Purple). The starting dilution of antibody was 45μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Sodium Carbonate blocking buffer, Mouse Anti-6X HIS Epitope Tag HRP conjugated Antibody and TMB substrate.

• WB

Supplier Data

Western blot - Anti-Digoxigenin antibody [DIG45] (AB288734)

Western Blot of Anti-DIG Single Domain Antibody. Lane 1 : Prestained Molecular Weight Marker (5μL). Lane 2 : DIG-BSA conjugated (0.2μg). Lane 3 : BSA only (0.2μg). Lane 4 : HEK293T lysate (20μg). Primary Antibody : Anti-DIG (VhH) at 1μg/mL incubated overnight at 4°C. Secondary Antibody : Mouse Anti-6X HIS DyLight™649 at 1 : 1000 incubated for 1hr at RT. Blocking buffer : Universal Buffer for 2hrs at RT. Expected MW : ~80kDa in DIG+BSA. Observed MW : ~80kDa in DIG+BSA (arrow); secondary background seen in HEK293T Lysate.

All lanes:

Western blot - Anti-Digoxigenin antibody [DIG45] (ab288734)

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