Anti-Dihydrofolate reductase (DHFR) antibody [9B2]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [9B2] (AB288373)

IHC image of ab288373 diluted at 1 : 100 and staining in paraffin-embedded human liver cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [9B2] (AB288373)

IHC image of ab288373 diluted at 1 : 100 and staining in paraffin-embedded human breast cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

• IP

Supplier Data

Immunoprecipitation - Anti-Dihydrofolate reductase (DHFR) antibody [9B2] (AB288373)

Immunoprecipitating DHFR in HeLa whole cell lysate.
Lane 1 : Rabbit control IgG instead of ab288373 in HeLa whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2 : ab288373 (2 μg)+ HeLa whole cell lysate (500 μg)
Lane 3 : HeLa whole cell lysate (10 μg)

All lanes:

Immunoprecipitation - Anti-Dihydrofolate reductase (DHFR) antibody [9B2] (ab288373)

Predicted band size: 21 kDa

false

• WB

Supplier Data

Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [9B2] (AB288373)

All lanes:

Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [9B2] (ab288373) at 1/2000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 3:

K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate

Lane 4:

Raji (Human Burkitt's lymphoma cell line) whole cell lysate

Lane 5:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 6:

Rat heart tissue

Lane 7:

Mouse kidney tissue

Lane 8:

Mouse liver tissue

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 21 kDa

Observed band size: 22 kDa

false