Anti-Dihydrofolate reductase (DHFR) antibody [9B2]

4 product images

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  Immunoprecipitation - Anti-Dihydrofolate reductase (DHFR) antibody [9B2] (ab288373)

  Immunoprecipitating DHFR in HeLa whole cell lysate.
  Lane 1: Rabbit control IgG instead of ab288373 in HeLa whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
  Lane 2: ab288373 (2 μg)+ HeLa whole cell lysate (500 μg)
  Lane 3: HeLa whole cell lysate (10 μg)

  All lanes: Immunoprecipitation - Anti-Dihydrofolate reductase (DHFR) antibody [9B2] (ab288373)

  Predicted band size: 21 kDa

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  Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [9B2] (ab288373)

  All lanes: Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [9B2] (ab288373) at 1/2000 dilution

  Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

  Lane 2: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

  Lane 3: K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate

  Lane 4: Raji (Human Burkitt's lymphoma cell line) whole cell lysate

  Lane 5: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

  Lane 6: Rat heart tissue

  Lane 7: Mouse kidney tissue

  Lane 8: Mouse liver tissue

  Secondary

  All lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution

  Predicted band size: 21 kDa

  Observed band size: 22 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [9B2] (ab288373)

  IHC image of ab288373 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [9B2] (ab288373)

  IHC image of ab288373 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.