AB133546

Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284]

RabMAb
Recombinant
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(9 Publications)

Rabbit Recombinant Monoclonal Dihydrofolate reductase (DHFR) antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 9 publications.

View Alternative Names

Dihydrofolate reductase, DHFR

3 Images

Lab

Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (AB133546)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546) at 1/10000 dilution

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 2:

U937 (Human histiocytic lymphoma cell line) whole cell lysate at 20 µg

Lane 3:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Lane 4:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 21 kDa,28 kDa,57 kDa,9 kDa

Observed band size: 21 kDa

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Unknown

Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (AB133546)

All lanes:

Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

U937 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Predicted band size: 21 kDa

false

OI-RD Scanning - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (AB133546)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (AB133546)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5284

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reduction of 7,8-dihydrofolate (DHF) to 5,6,7,8-tetrahydrofolate in a NADPH-dependent manner (PubMed : 12096917, PubMed : 15039552, PubMed : 17569517, PubMed : 19196009, PubMed : 19478082, PubMed : 21876184, PubMed : 9719595). Key enzyme in folate metabolism. Contributes to the nuclear and mitochondrial de novo thymidylate biosynthesis pathway (PubMed : 21876188, PubMed : 22235121). Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

See full target information Dihydrofolate reductase

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1209490 PubMed37457690

2023

The actions of methotrexate on endothelial cells are dependent on the shear stress-induced regulation of one carbon metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Marie B Lang,Kit-Yi Leung,Nicholas D E Greene,Kerri M Malone,Gaye Saginc,Anna M Randi,Allan Kiprianos,Robert T Maughan,Charis Pericleous,Justin C Mason

International journal of molecular sciences 22: PubMed34502300

2021

MTHFR Knockdown Assists Cell Defense against Folate Depletion Induced Chromosome Segregation and Uracil Misincorporation in DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Tsung Wu,Wei-Ting Ye,Yi-Cheng Wang,Po-Ming Chen,Jun-You Liu,Chien-Kuo Tai,Feng-Yao Tang,Jian-Rong Li,Chun-Chi Liu,En-Pei Isabel Chiang

The Journal of cell biology 220: PubMed33819341

2021

miR-146 connects stem cell identity with metabolism and pharmacological resistance in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara Tordonato,Matteo Jacopo Marzi,Giovanni Giangreco,Stefano Freddi,Paola Bonetti,Daniela Tosoni,Pier Paolo Di Fiore,Francesco Nicassio

Biotechnology journal 16:e2000351 PubMed33314785

2020

Comprehensive characterization of dihydrofolate reductase-mediated gene amplification for the establishment of recombinant human embryonic kidney 293 cells producing monoclonal antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Sang Yoon Lee,Minhye Baek,Gyun Min Lee

Cell death & disease 10:327 PubMed30975976

2019

The mevalonate coordinates energy input and cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Li Gong,Yi Xiao,Fan Xia,Pei Wu,Tingting Zhao,Shulin Xie,Ran Wang,Qiaocheng Wen,Wensu Zhou,Huilan Xu,Lingyan Zhu,Zeqi Zheng,Tianlun Yang,Zihua Chen,Qiong Duan

Molecular cancer research : MCR 15:189-200 PubMed28108628

2017

Metabolic Reprogramming by Folate Restriction Leads to a Less Aggressive Cancer Phenotype.

Applications

Species

Mouse

Zahra Ashkavand,Ciara O'Flanagan,Mirko Hennig,Xiuxia Du,Stephen D Hursting,Sergey A Krupenko

Molecular and cellular biology 36:1222-36 PubMed26830229

2016

Arrested Hematopoiesis and Vascular Relaxation Defects in Mice with a Mutation in Dhfr.

Applications

Unspecified application

Species

Unspecified reactive species

Julie A I Thoms,Kathy Knezevic,Jia Jenny Liu,Elias N Glaros,Thuan Thai,Qiao Qiao,Heather Campbell,Deborah Packham,Yizhou Huang,Peter Papathanasiou,Robert Tunningley,Belinda Whittle,Amanda W S Yeung,Vashe Chandrakanthan,Luke Hesson,Vivien Chen,Jason W H Wong,Louise E Purton,Robyn L Ward,Shane R Thomas,John E Pimanda

Stem cell reviews and reports 12:340-51 PubMed26815725

2016

Disparate Response to Methotrexate in Stem Versus Non-Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Olivia S Beane,Louise E O Darling,Vera C Fonseca,Eric M Darling

Experimental and molecular pathology 95:83-90 PubMed23726796

2013

Overexpression of thymidylate synthetase confers an independent prognostic indicator in nasopharyngeal carcinoma.

Applications

IHC

Species

Human

Sung-Wei Lee,Tzu-Ju Chen,Li-Ching Lin,Chien-Feng Li,Li-Tzong Chen,Chung-Hsi Hsing,Han-Ping Hsu,Chia-Jung Tsai,Hsuan-Ying Huang,Yow-Ling Shiue

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284]

Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (AB133546)

Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (AB133546)

OI-RD Scanning - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (AB133546)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (9)

Product promise