Rabbit Recombinant Monoclonal Dihydrofolate reductase (DHFR) antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 9 publications.
Images
![Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (AB133546), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5284-ab133546--western-blot-img110522.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (AB133546)")
![Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (AB133546), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5284-ab133546--western-blot-img116079.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (AB133546)")
![OI-RD Scanning - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (AB133546), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5284-ab133546--oi-rd-scanning-img95378.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (AB133546)")
Publications
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- Frontiers in immunology 14:12094902023The actions of methotrexate on endothelial cells are dependent on the shear stress-induced regulation of one carbon metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie B Lang,Kit-Yi Leung,Nicholas D E Greene,Kerri M Malone,Gaye Saginc,Anna M Randi,Allan Kiprianos,Robert T Maughan,Charis Pericleous,Justin C MasonPubMed 37457690
- International journal of molecular sciences 22:2021MTHFR Knockdown Assists Cell Defense against Folate Depletion Induced Chromosome Segregation and Uracil Misincorporation in DNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing-Tsung Wu,Wei-Ting Ye,Yi-Cheng Wang,Po-Ming Chen,Jun-You Liu,Chien-Kuo Tai,Feng-Yao Tang,Jian-Rong Li,Chun-Chi Liu,En-Pei Isabel ChiangPubMed 34502300
- The Journal of cell biology 220:2021miR-146 connects stem cell identity with metabolism and pharmacological resistance in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiara Tordonato,Matteo Jacopo Marzi,Giovanni Giangreco,Stefano Freddi,Paola Bonetti,Daniela Tosoni,Pier Paolo Di Fiore,Francesco NicassioPubMed 33819341
- Biotechnology journal 16:e20003512020Comprehensive characterization of dihydrofolate reductase-mediated gene amplification for the establishment of recombinant human embryonic kidney 293 cells producing monoclonal antibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSang Yoon Lee,Minhye Baek,Gyun Min LeePubMed 33314785
- Cell death & disease 10:3272019The mevalonate coordinates energy input and cell proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Gong,Yi Xiao,Fan Xia,Pei Wu,Tingting Zhao,Shulin Xie,Ran Wang,Qiaocheng Wen,Wensu Zhou,Huilan Xu,Lingyan Zhu,Zeqi Zheng,Tianlun Yang,Zihua Chen,Qiong DuanPubMed 30975976
- Molecular cancer research : MCR 15:189-2002017Metabolic Reprogramming by Folate Restriction Leads to a Less Aggressive Cancer Phenotype.Applications:WBReactive species:MouseZahra Ashkavand,Ciara O'Flanagan,Mirko Hennig,Xiuxia Du,Stephen D Hursting,Sergey A KrupenkoPubMed 28108628
- Molecular and cellular biology 36:1222-362016Arrested Hematopoiesis and Vascular Relaxation Defects in Mice with a Mutation in Dhfr.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulie A I Thoms et. al.PubMed 26830229
- Stem cell reviews and reports 12:340-512016Disparate Response to Methotrexate in Stem Versus Non-Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlivia S Beane,Louise E O Darling,Vera C Fonseca,Eric M DarlingPubMed 26815725
- Experimental and molecular pathology 95:83-902013Overexpression of thymidylate synthetase confers an independent prognostic indicator in nasopharyngeal carcinoma.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
Alternative names
Dihydrofolate reductase, DHFR
Recommended products
Rabbit Recombinant Monoclonal Dihydrofolate reductase (DHFR) antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 9 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR5284
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.28 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5284-ab133546--western-blot-img110522.jpg "Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546)")
Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546) at 1/10000 dilution
Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Lane 2: U937 (Human histiocytic lymphoma cell line) whole cell lysate at 20 µg
Lane 3: C6 (Rat glial tumor cell line) whole cell lysate at 20 µg
Lane 4: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg
Lane 5: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 21 kDa, 28 kDa, 57 kDa, 9 kDa
Observed band size: 21 kDa
![Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5284-ab133546--western-blot-img116079.jpg "Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546)")
Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546)
All lanes: Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: U937 cell lysate at 10 µg
Lane 4: 293T cell lysate at 10 µg
Predicted band size: 21 kDa
![OI-RD Scanning - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5284-ab133546--oi-rd-scanning-img95378.jpg "OI-RD Scanning - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546)")
OI-RD Scanning - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Downloads
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- Visit the general protocols
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