Rabbit Recombinant Monoclonal Dihydrofolate reductase (DHFR) antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.
Images
![Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (AB248545), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5284-bsa-and-azide-free-ab248545--western-blot-img110522.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (AB248545)")
![Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (AB248545), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5284-bsa-and-azide-free-ab248545--western-blot-img116079.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (AB248545)")
![OI-RD Scanning - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (AB248545), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5284-bsa-and-azide-free-ab248545--oi-rd-scanning-img95378.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (AB248545)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
Alternative names
Dihydrofolate reductase, DHFR
Recommended products
Rabbit Recombinant Monoclonal Dihydrofolate reductase (DHFR) antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR5284
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.28 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248545 is the carrier-free version of Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] ab133546.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (ab248545), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5284-bsa-and-azide-free-ab248545--western-blot-img110522.jpg "Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (ab248545)")
Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (ab248545)
This data was developed using Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] ab133546, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] ab133546) at 1/10000 dilution
Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Lane 2: U937 (Human histiocytic lymphoma cell line) whole cell lysate at 20 µg
Lane 3: C6 (Rat glial tumor cell line) whole cell lysate at 20 µg
Lane 4: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg
Lane 5: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 21 kDa, 28 kDa, 57 kDa, 9 kDa
Observed band size: 21 kDa
![Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (ab248545), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5284-bsa-and-azide-free-ab248545--western-blot-img116079.jpg "Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (ab248545)")
Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (ab248545)
This data was developed using Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] ab133546, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] ab133546) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: U937 cell lysate at 10 µg
Lane 4: 293T cell lysate at 10 µg
Predicted band size: 21 kDa
![OI-RD Scanning - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (ab248545), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5284-bsa-and-azide-free-ab248545--oi-rd-scanning-img95378.jpg "OI-RD Scanning - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (ab248545)")
OI-RD Scanning - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] - BSA and Azide free (ab248545)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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