Rabbit Recombinant Monoclonal Dihydrofolate reductase (DHFR) antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 10 publications.
Images
![Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (AB124814), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5285-ab124814--western-blot-img169296.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (AB124814)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (AB124814), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5285-ab124814--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img168270.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (AB124814)")
Publications
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- Frontiers in molecular neuroscience 16:11219442023Increased NOX2 expression in astrocytes leads to eNOS uncoupling through dihydrofolate reductase in endothelial cells after subarachnoid hemorrhage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu-Hao Miao,Sheng-Qing Gao,Hui-Xin Li,Yun-Song Zhuang,Xue Wang,Tao Li,Chao-Chao Gao,Yan-Ling Han,Jia-Yin Qiu,Meng-Liang ZhouPubMed 37063365
- Molecular cancer research : MCR 21:411-4272023BAP1 Loss Is Associated with Higher ASS1 Expression in Epithelioid Mesothelioma: Implications for Therapeutic Stratification.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah E Barnett,Jenna Kenyani,Martina Tripari,Zohra Butt,Rudi Grosman,Francesca Querques,Liam Shaw,Luisa C Silva,Zoe Goate,Stefan J Marciniak,Doris M Rassl,Richard Jackson,Lu-Yun Lian,Peter W Szlosarek,Joseph J Sacco,Judy M CoulsonPubMed 36669126
- Cellular and molecular life sciences : CMLS 79:5532022A plant-based mutant huntingtin model-driven discovery of impaired expression of GTPCH and DHFR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiu-Yueh Hung,Chuanshu Zhu,Farooqahmed S Kittur,Maotao He,Erland Arning,Jianhui Zhang,Asia J Johnson,Gurpreet S Jawa,Michelle D Thomas,Tomas T Ding,Jiahua XiePubMed 36251090
- Frontiers in pharmacology 12:7186752021Upregulation of Thymidylate Synthase Induces Pemetrexed Resistance in Malignant Pleural Mesothelioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuzo Sato,Masaru Tomita,Tomoyoshi Soga,Atsushi Ochiai,Hideki MakinoshimaPubMed 34646134
- Virus research 302:1984692021The folate antagonist methotrexate diminishes replication of the coronavirus SARS-CoV-2 and enhances the antiviral efficacy of remdesivir in cell culture models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKim M Stegmann,Antje Dickmanns,Sabrina Gerber,Vella Nikolova,Luisa Klemke,Valentina Manzini,Denise Schlösser,Cathrin Bierwirth,Julia Freund,Maren Sitte,Raimond Lugert,Gabriela Salinas,Toni Luise Meister,Stephanie Pfaender,Dirk Görlich,Bernd Wollnik,Uwe Groß,Matthias DobbelsteinPubMed 34090962
- The Journal of nutrition 150:1705-17122020p53 Disruption Increases Uracil Accumulation in DNA of Murine Embryonic Fibroblasts and Leads to Folic Acid-Nonresponsive Neural Tube Defects in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErica R Lachenauer,Sally P Stabler,Martha S Field,Patrick J StoverPubMed 32271909
- Acta neuropathologica communications 6:152018Clinical significance of polyglutamylation in primary central nervous system lymphoma.
- FEBS letters 589:1855-622015An active second dihydrofolate reductase enzyme is not a feature of rat and mouse, but they do have activity in their mitochondria.
- Cancer research 74:896-9072013Prognostic and therapeutic impact of argininosuccinate synthetase 1 control in bladder cancer as monitored longitudinally by PET imaging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael D Allen,Phuong Luong,Chantelle Hudson,Julius Leyton,Barbara Delage,Essam Ghazaly,Rosalind Cutts,Ming Yuan,Nelofer Syed,Cristiana Lo Nigro,Laura Lattanzio,Malgorzata Chmielewska-Kassassir,Ian Tomlinson,Rebecca Roylance,Hayley C Whitaker,Anne Y Warren,David Neal,Christian Frezza,Luis Beltran,Louise J Jones,Claude Chelala,Bor-Wen Wu,John S Bomalaski,Robert C Jackson,Yong-Jie Lu,Tim Crook,Nicholas R Lemoine,Stephen Mather,Julie Foster,Jane Sosabowski,Norbert Avril,Chien-Feng Li,Peter W SzlosarekPubMed 24285724
- Molecular cancer therapeutics 12:890-9002013The HSP90 inhibitor NVP-AUY922 potently inhibits non-small cell lung cancer growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEdward B Garon,Richard S Finn,Habib Hamidi,Judy Dering,Sharon Pitts,Naeimeh Kamranpour,Amrita J Desai,Wylie Hosmer,Susan Ide,Emin Avsar,Michael Rugaard Jensen,Cornelia Quadt,Manway Liu,Steven M Dubinett,Dennis J SlamonPubMed 23493311
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Expected | Not recommended | Not recommended | Tested |
| Rat | Expected | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes (Heat to 98°C, allow to cool for 10-20 minutes) The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10000 - 1/50000 | Notes - |
Species Rat | Dilution info 1/10000 - 1/50000 | Notes - |
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
Alternative names
Dihydrofolate reductase, DHFR
Recommended products
Rabbit Recombinant Monoclonal Dihydrofolate reductase (DHFR) antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 10 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR5285
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5285-ab124814--western-blot-img169296.jpg "Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814)")
Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814)
All lanes: Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814) at 1/10000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 3: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 4: Y79 (Human retinoblastoma retinoblastoma) whole cell lysate
Lane 5: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 6: Mouse spleen lysate
Lane 7: Mouse kidney lysate
Lane 8: Rat kidney lysate
Lane 9: PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
SecondaryLanes 1 - 7: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Lanes 8 - 9: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
Predicted band size: 21 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5285-ab124814--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img168270.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial cancer tissue sections labeling Dihydrofolate reductase (DHFR) with purified ab124814 at 1/1000 dilution (0.20 μg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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