Rabbit Recombinant Monoclonal Dihydrofolate reductase (DHFR) antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] - BSA and Azide free (AB248017), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5285-bsa-and-azide-free-ab248017--western-blot-img169296.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] - BSA and Azide free (AB248017)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] - BSA and Azide free (AB248017), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5285-bsa-and-azide-free-ab248017--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img168270.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] - BSA and Azide free (AB248017)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Expected | Not recommended | Not recommended | Tested |
| Rat | Expected | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
Alternative names
Dihydrofolate reductase, DHFR
Recommended products
Rabbit Recombinant Monoclonal Dihydrofolate reductase (DHFR) antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR5285
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248017 is the carrier-free version of Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] ab124814.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] - BSA and Azide free (ab248017), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5285-bsa-and-azide-free-ab248017--western-blot-img169296.jpg "Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] - BSA and Azide free (ab248017)")
Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] - BSA and Azide free (ab248017)
All lanes: Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] ab124814) at 1/10000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 3: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 4: Y79 (Human retinoblastoma retinoblastoma) whole cell lysate
Lane 5: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 6: Mouse spleen lysate
Lane 7: Mouse kidney lysate
Lane 8: Rat kidney lysate
Lane 9: PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
SecondaryLanes 1 - 7: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Lanes 8 - 9: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
Predicted band size: 21 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] - BSA and Azide free (ab248017), expandable thumbnail](https://content.abcam.com/products/images/dihydrofolate-reductase-dhfr-antibody-epr5285-bsa-and-azide-free-ab248017--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img168270.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] - BSA and Azide free (ab248017)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] - BSA and Azide free (ab248017)
This data was developed using Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] ab124814, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial cancer tissue sections labeling Dihydrofolate reductase (DHFR) with purified Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] ab124814 at 1/1000 dilution (0.20 μg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com