AB413

Anti-dimethyl Arginine antibody [21C7]

Name: Anti-dimethyl Arginine antibody [21C7] Mouse monoclonal (ab413) | Abcam
Brand: Abcam Limited
SKU: AB413
Rating: 5 (2 reviews)

(2 Reviews)

(12 Publications)

Mouse Monoclonal dimethyl Arginine antibody. Suitable for IP, ChIP, WB and reacts with Modified Amino Acid samples. Cited in 12 publications.

View Alternative Names

Di methyl Arginine

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

21C7

Isotype

IgM

Light chain type

kappa

Carrier free

Applications

WB, IP, ChIP

applications

Specificity

This antibody reacts with free and bound asymmetric N^G-N^G-dimethyl arginine. It is not known whether the antibody reacts with symmetric dimethyl arginine.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "Use 5µl for 4x106 cells.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/50 - 1/471", "WB-species-notes": "" } } }

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Product details

This antibody will be of use in chromatin and transcription factor research to monitor methylation status. Since it is specific to dimethyl arginine it can be used in parallel with ab412 (detects monomethyl and dimethyl arginine) and ab414 (detects monomethyl arginine only) to monitor the exact modification status.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dimethyl Arginine often referred to as asymmetric dimethylarginine (ADMA) is an endogenously produced molecule in the body. The molecular mass of dimethyl arginine usually depends on its specific dimethylated form with the molecule having different variants such as symmetric dimethylarginine (SDMA) and monomethylarginine (MMA). While dimethyl arginine is expressed in various tissues it predominantly arises as a byproduct of protein methylation processes. Protein arginine methyltransferases (PRMTs) enzymes responsible for these modifications mediate its formation influencing numerous physiological functions.

Biological function summary

Dimethyl arginine modulates nitric oxide (NO) signaling by inhibiting nitric oxide synthase (NOS) enzymes which are vital for the production of NO—an important player in vasodilation and immune response. NO production requires L-arginine as a substrate and competition from dimethylated arginines reduces NO availability. This molecule though not a part of any large complex significantly impacts endothelial function and cell proliferation by affecting NO synthesis and cellular response to growth factors.

Pathways

Dimethyl arginine plays an integral role in the regulation of the cardiovascular system and is involved in the urea cycle and polyamine pathways which are important for removing excess nitrogen and supporting cell growth respectively. The enzymes related to these pathways such as endothelial nitric oxide synthase (eNOS) and dimethylarginine dimethylaminohydrolase (DDAH) illustrate the interconnectedness of dimethyl arginine in maintaining vascular health and cellular homeostasis. The balance between dimethyl arginine and arginine levels affect the efficiency of the pathways indicating their influence on systemic health.

Dimethyl arginine links to cardiovascular diseases and chronic kidney disease. Its elevated levels often point to endothelial dysfunction contributing to the pathogenesis of atherosclerosis due to impaired NO-mediated vasodilation. Additionally kidney dysfunction can result in decreased dimethylarginine clearance exacerbating cardiovascular issues. Proteins such as eNOS and DDAH play pivotal roles in these conditions as they modulate the effects of dimethyl arginine on vascular and kidney health highlighting the importance of managing its levels in disease prevention and treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information dimethyl Arginine

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:375 PubMed38548869

2024

The protein methyltransferase TrSAM inhibits cellulase gene expression by interacting with the negative regulator ACE1 in Trichoderma reesei.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihua Zhu,Gen Zou,Shunxing Chai,Meili Xiao,Yinmei Wang,Pingping Wang,Zhihua Zhou

Science advances 9:eadi2232 PubMed37682999

2023

Vitamin B is governed by the local compartmentalization of metabolic enzymes during growth.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina N Franco,Laurence J Seabrook,Steven T Nguyen,Ying Yang,Melissa Campos,Qi Fan,Andrew C Cicchetto,Mei Kong,Heather R Christofk,Lauren V Albrecht

Frontiers in cellular neuroscience 13:275 PubMed31293390

2019

Electroacupuncture Pretreatment Ameliorates PTSD-Like Behaviors in Rats by Enhancing Hippocampal Neurogenesis via the Keap1/Nrf2 Antioxidant Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Cui-Hong Zhou,Fen Xue,Shan-Shan Xue,Han-Fei Sang,Ling Liu,Ying Wang,Min Cai,Zhang-Jin Zhang,Qing-Rong Tan,Hua-Ning Wang,Zheng-Wu Peng

Proceedings of the National Academy of Sciences of 115:E5317-E5325 PubMed29773710

2018

Arginine methylation is required for canonical Wnt signaling and endolysosomal trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren V Albrecht,Diego Ploper,Nydia Tejeda-Muñoz,Edward M De Robertis

Nature communications 6:8922 PubMed26568365

2015

Mycobacteria modulate host epigenetic machinery by Rv1988 methylation of a non-tail arginine of histone H3.

Applications

Unspecified application

Species

Unspecified reactive species

Imtiyaz Yaseen,Prabhjot Kaur,Vinay Kumar Nandicoori,Sanjeev Khosla

Science signaling 6:ra104 PubMed24300896

2013

Lysine methylation promotes VEGFR-2 activation and angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Edward J Hartsough,Rosana D Meyer,Vipul Chitalia,Yan Jiang,Victor E Marquez,Irina V Zhdanova,Janice Weinberg,Catherine E Costello,Nader Rahimi

Biopolymers 101:133-43 PubMed23576281

2013

Chemical and biological methods to detect post-translational modifications of arginine.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel J Slade,Venkataraman Subramanian,Jakob Fuhrmann,Paul R Thompson

Journal of virology 83:5309-20 PubMed19321610

2009

Arginine methylation of the ICP27 RGG box regulates ICP27 export and is required for efficient herpes simplex virus 1 replication.

Applications

Species

Unspecified reactive species

Stuart K Souki,Paul D Gershon,Rozanne M Sandri-Goldin

Biochemistry 45:10385-93 PubMed16922515

2006

Alternative splicing modulates protein arginine methyltransferase-dependent methylation of fragile X syndrome mental retardation protein.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Dolzhanskaya,George Merz,Robert B Denman

Molecular and cellular biology 25:5648-63 PubMed15964820

2005

Arginine methylation provides epigenetic transcription memory for retinoid-induced differentiation in myeloid cells.

Applications

ChIP

Species

Human

Balint L Balint,Attila Szanto,Andras Madi,Uta-Maria Bauer,Petra Gabor,Szilvia Benko,Laszlo G Puskás,Peter J A Davies,Laszlo Nagy

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-dimethyl Arginine antibody [21C7]

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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