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AB184978

Anti-DIMT1L antibody

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(2 Publications)

Rabbit Polyclonal DIMT1L antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DIMT1 aa 1-150.

View Alternative Names

DIMT1L, HUSSY-05, DIMT1, Dimethyladenosine transferase, 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase, 18S rRNA dimethylase, DIM1 dimethyladenosine transferase 1 homolog, DIM1 dimethyladenosine transferase 1-like

5 Images
Immunocytochemistry - Anti-DIMT1L antibody (AB184978)
  • ICC

Supplier Data

Immunocytochemistry - Anti-DIMT1L antibody (AB184978)

Immunofluorescence analysis of PFA/Triton X-100-fixed U-251 MG cells labeling DIMT1L with ab184978 at 4 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIMT1L antibody (AB184978)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIMT1L antibody (AB184978)

Immunohistochemical analysis of paraffin-embedded Human hippocampus tissue labeling DIMT1L with ab184978 at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIMT1L antibody (AB184978)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIMT1L antibody (AB184978)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling DIMT1L with ab184978 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIMT1L antibody (AB184978)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIMT1L antibody (AB184978)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human squamous epithelia tissue labelling DIMT1L with ab184978 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-DIMT1L antibody (AB184978)
  • WB

Unknown

Western blot - Anti-DIMT1L antibody (AB184978)

All lanes:

Western blot - Anti-DIMT1L antibody (ab184978) at 0.4 µg/mL

All lanes:

RPMI 8226 (human lymphoblast cell line) cell lysate

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC

applications

Immunogen

Recombinant Fragment Protein within Human DIMT1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9UNQ2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.25-2 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DIMT1L also known as DIMT1 or Nsun5 is a 58 kDa protein involved in ribosomal RNA methylation. It functions as a methyltransferase introducing methyl groups at specific adenine residues in the small subunit ribosomal RNA (rRNA). The protein shows expression mainly in tissues with high rates of protein synthesis such as liver kidney and pancreas reflecting its role in ribosome biogenesis. Its widespread expression in proliferative tissues highlights its importance for cellular growth and division.
Biological function summary

The protein DIMT1L plays an important role in assembling the 40S ribosomal subunit a part of the eukaryotic 80S ribosome. As a methyltransferase this protein methylates adenine residues in rRNA influencing the ribosome's structural and functional integrity. It usually functions as a monomer not requiring large complexes to exert its effects. This methyltransferase activity is important for correct ribosome maturation which in turn affects protein synthesis capacity within cells.

Pathways

DIMT1L activity is integral to ribosome biogenesis and rRNA processing pathways. The protein interacts with components of the ribonucleoprotein complex to facilitate the final maturation steps of the 40S subunit. It associates with proteins like Nop14 which participate in similar rRNA processing pathways ensuring the fidelity of ribosome assembly. Its role in these pathways highlights the importance of ribosomal integrity for effective translation and overall cellular function.

Research links DIMT1L to certain cancers and growth defects. Aberrations in its expression or function can lead to dysregulation in ribosome production contributing to tumorigenesis. The protein sometimes connects to pathways involving tumor suppressor proteins like p53 where disruption can accelerate cancer progression. Beyond cancer other proliferative disorders might arise from alterations in DIMT1L-mediated processes highlighting the need for further exploration into its mechanistic impacts on human health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle (PubMed : 25851604). Involved in the pre-rRNA processing steps leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity (PubMed : 25851604). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).
See full target information DIMT1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 27:25 PubMed38125354

2023

Identification of potential miR‑155 target genes in epidermal immune microenvironment of atopic dermatitis patients and their inflammatory effects on HaCaT cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochen Wang,Lu Chen,Xiaoqing Chen,Chang Liu,Wenhong Qiu,Kaiwen Guo

The Journal of biological chemistry 295:12058-12070 PubMed32616653

2020

Structural and catalytic roles of the human 18 rRNA methyltransferases DIMT1 in ribosome assembly and translation.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Shen,Julian Stoute,Kathy Fange Liu
View all publications
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Product promise

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