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AB77481

Anti-DIO2 antibody

5

(2 Reviews)

|

(10 Publications)

Goat Polyclonal DIO2 antibody. Suitable for ICC, IHC-Fr, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human DIO2.

View Alternative Names

ITDI2, TXDI2, DIO2, Type II iodothyronine deiodinase, 5DII, DIOII, Type 2 DI, Type-II 5'-deiodinase

3 Images
Flow Cytometry (Intracellular) - Anti-DIO2 antibody (AB77481)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DIO2 antibody (AB77481)

Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr at 10 μg/mL followed by Alexa Fluor 488 secondary antibody at 1 μg/mL. IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry - Anti-DIO2 antibody (AB77481)
  • ICC

Supplier Data

Immunocytochemistry - Anti-DIO2 antibody (AB77481)

Immunocytochemistry analysis of HeLa cells labelling DIO2 with ab77481 at 10 μg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.15% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 2 μg/mLwas used as the secondary antibody (green) showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry (Frozen sections) - Anti-DIO2 antibody (AB77481)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-DIO2 antibody (AB77481)

ab77481 staining DIO2 in mouse Brain (Hippocampus) tissue section by Immunohistochemistry (Frozen sections). Tissue underwent antigen retrieval in microwave with citrate buffer (pH 4.5). The HRP-staining procedure was used for detection.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-Fr, ICC, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human DIO2. The exact immunogen used to generate this antibody is proprietary information.

Q92813

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'DIO2' also known as Type II iodothyronine deiodinase is an enzyme responsible for the conversion of thyroxine (T4) to the active hormone triiodothyronine (T3) by removal of an iodine atom. This transformation is important for regulating thyroid hormone activity. DIO2 has a molecular mass of approximately 58 kDa and is expressed predominantly in tissues such as the brain pituitary gland brown adipose tissue and skeletal muscle. It plays an integral role in maintaining intracellular T3 levels necessary for various physiological functions.
Biological function summary

DIO2 modulates local T3 availability which impacts metabolic rate thermogenesis and development. DIO2 is not part of a protein complex but interacts closely with thyroid hormone receptors that are activated by the produced T3. In adipocytes and myocytes DIO2-mediated T3 generation ensures the hormones' optimal action affecting processes such as energy expenditure and muscle function. This activity is critical in adapting to changing metabolic demands.

Pathways

DIO2 is integral to the thyroid hormone signaling pathway significantly influencing processes governed by thyroid hormone function. By converting T4 to T3 DIO2 interacts with thyroid hormone receptors and influences downstream effectors linked to metabolism and growth. DIO2 activity is regulated via the hypothalamic-pituitary-thyroid axis ensuring proper feedback loops and homeostasis. Additionally it is related to other deiodinases like DIO1 and DIO3 which also participate in thyroid hormone modification.

Alterations in DIO2 expression or activity have been linked to thyroid-related conditions such as hypothyroidism and Graves' disease. In hypothyroidism decreased DIO2 activity could lead to reduced local T3 levels affecting cellular metabolism. In Graves' disease overactive DIO2 may result in excessive T3 production exacerbating symptoms. DIO2's action further connects with thyroid hormone receptor beta (THRB) where abnormalities can contribute to resistance to thyroid hormones influencing disease progression and treatment outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.
See full target information DIO2
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in nutrition 9:1012362 PubMed36185664

2022

Effects of insufficient serine on health and selenoprotein expression in rats and their offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqun Liu,Jianrong Wang,Qin Wang,Feng Han,Lili Shi,Chao Han,Zhenwu Huang,Liang Xu

Autophagy 18:2427-2442 PubMed35196191

2022

Defective autophagy contributes to endometrial epithelial-mesenchymal transition in intrauterine adhesions.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhua Zhou,Huiyan Wang,Xiwen Zhang,Minmin Song,Simin Yao,Peipei Jiang,Dan Liu,Zhiyin Wang,Haining Lv,Ruotian Li,Ying Hong,Jianwu Dai,Yali Hu,Guangfeng Zhao

Nature communications 12:6845 PubMed34824246

2021

Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ting Chen,Qi-Yuan Yang,Yun Hu,Xiang-Dong Liu,Jeanene M de Avila,Mei-Jun Zhu,Peter W Nathanielsz,Min Du

Science bulletin 66:478-489 PubMed33936858

2021

Embryonic exposure to hyper glucocorticoids suppresses brown fat development and thermogenesis via REDD1.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ting Chen,Yun Hu,Qi-Yuan Yang,Xiang-Dong Liu,Jun Seok Son,Jeanene M de Avila,Mei-Jun Zhu,Min Du

Nature communications 11:2551 PubMed32439985

2020

FoxO1-Dio2 signaling axis governs cardiomyocyte thyroid hormone metabolism and hypertrophic growth.

Applications

Unspecified application

Species

Unspecified reactive species

Anwarul Ferdous,Zhao V Wang,Yuxuan Luo,Dan L Li,Xiang Luo,Gabriele G Schiattarella,Francisco Altamirano,Herman I May,Pavan K Battiprolu,Annie Nguyen,Beverly A Rothermel,Sergio Lavandero,Thomas G Gillette,Joseph A Hill

PloS one 13:e0199682 PubMed29965978

2018

Increased hydrophilic plasma bile acids are correlated with protection from adiposity in skin-specific stearoyl-CoA desaturase-1 deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina N Dumas,James M Ntambi

The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians 32:2746-2757 PubMed29557697

2018

Maternal and placental responses before preterm birth: adaptations to increase fetal thyroid hormone availability?

Applications

Unspecified application

Species

Unspecified reactive species

An Eerdekens,Lies Langouche,Fabian Güiza,Johan Verhaeghe,Gunnar Naulaers,Christine Vanhole,Greet Van den Berghe

Nature medicine 24:39-49 PubMed29200204

2017

Thyroid hormone inhibits lung fibrosis in mice by improving epithelial mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Guoying Yu,Argyris Tzouvelekis,Rong Wang,Jose D Herazo-Maya,Gabriel H Ibarra,Anup Srivastava,Joao Pedro Werneck de Castro,Giuseppe DeIuliis,Farida Ahangari,Tony Woolard,Nachelle Aurelien,Rafael Arrojo E Drigo,Ye Gan,Morven Graham,Xinran Liu,Robert J Homer,Thomas S Scanlan,Praveen Mannam,Patty J Lee,Erica L Herzog,Antonio C Bianco,Naftali Kaminski

Nature communications 8:14147 PubMed28128199

2017

A-FABP mediates adaptive thermogenesis by promoting intracellular activation of thyroid hormones in brown adipocytes.

Applications

WB

Species

Unspecified reactive species

Lingling Shu,Ruby L C Hoo,Xiaoping Wu,Yong Pan,Ida P C Lee,Lai Yee Cheong,Stefan R Bornstein,Xianglu Rong,Jiao Guo,Aimin Xu

Cardiovascular research 87:636-46 PubMed20453157

2010

Up-regulation of type 2 iodothyronine deiodinase in dilated cardiomyopathy.

Applications

WB

Species

Mouse

Yuan-Yuan Wang,Sachio Morimoto,Cheng-Kun Du,Qun-Wei Lu,Dong-Yun Zhan,Takaki Tsutsumi,Tomomi Ide,Yosikazu Miwa,Fumi Takahashi-Yanaga,Toshiyuki Sasaguri
View all publications
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