AB77779

Anti-DIO2 antibody

Name: Anti-DIO2 antibody (ab77779) | Abcam
Brand: Abcam Limited
SKU: AB77779
Rating: 1 (1 reviews)

(1 Review)

(12 Publications)

Rabbit Polyclonal DIO2 antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 12 publications.

View Alternative Names

ITDI2, TXDI2, DIO2, Type II iodothyronine deiodinase, 5DII, DIOII, Type 2 DI, Type-II 5'-deiodinase

2 Images

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Western blot - Anti-DIO2 antibody (AB77779)

All lanes:

Western blot - Anti-DIO2 antibody (ab77779) at 1 µg/mL

Lane 1:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Lane 2:

Human placenta tissue lysate - total protein (<a href='/en-us/products/unavailable/human-placenta-tissue-lysate-total-protein-ab29745'>ab29745</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 31 kDa

Observed band size: 105 kDa,31 kDa,55 kDa

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Western blot - Anti-DIO2 antibody (AB77779)

All lanes:

Western blot - Anti-DIO2 antibody (ab77779) at 1 µg/mL

Lane 1:

Mouse Retina Tissue Lysate at 10 µg

Lane 2:

Western blot - Mouse brain tissue lysate - total protein (0 days) (<a href='/en-us/products/tissue-lysates/mouse-brain-tissue-lysate-total-protein-0-days-ab7188'>ab7188</a>) at 10 µg

Lane 3:

Brain (Rat) Tissue Lysate at 10 µg

Lane 4:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) at 1/10000 dilution

Predicted band size: 31 kDa

Observed band size: 35 kDa,40 kDa,55 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Goat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'DIO2' also known as Type II iodothyronine deiodinase is an enzyme responsible for the conversion of thyroxine (T4) to the active hormone triiodothyronine (T3) by removal of an iodine atom. This transformation is important for regulating thyroid hormone activity. DIO2 has a molecular mass of approximately 58 kDa and is expressed predominantly in tissues such as the brain pituitary gland brown adipose tissue and skeletal muscle. It plays an integral role in maintaining intracellular T3 levels necessary for various physiological functions.

Biological function summary

DIO2 modulates local T3 availability which impacts metabolic rate thermogenesis and development. DIO2 is not part of a protein complex but interacts closely with thyroid hormone receptors that are activated by the produced T3. In adipocytes and myocytes DIO2-mediated T3 generation ensures the hormones' optimal action affecting processes such as energy expenditure and muscle function. This activity is critical in adapting to changing metabolic demands.

Pathways

DIO2 is integral to the thyroid hormone signaling pathway significantly influencing processes governed by thyroid hormone function. By converting T4 to T3 DIO2 interacts with thyroid hormone receptors and influences downstream effectors linked to metabolism and growth. DIO2 activity is regulated via the hypothalamic-pituitary-thyroid axis ensuring proper feedback loops and homeostasis. Additionally it is related to other deiodinases like DIO1 and DIO3 which also participate in thyroid hormone modification.

Alterations in DIO2 expression or activity have been linked to thyroid-related conditions such as hypothyroidism and Graves' disease. In hypothyroidism decreased DIO2 activity could lead to reduced local T3 levels affecting cellular metabolism. In Graves' disease overactive DIO2 may result in excessive T3 production exacerbating symptoms. DIO2's action further connects with thyroid hormone receptor beta (THRB) where abnormalities can contribute to resistance to thyroid hormones influencing disease progression and treatment outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.

See full target information DIO2

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Biological & pharmaceutical bulletin 48:267-278 PubMed40128937

2025

Estradiol Regulates the Expression of Type 3 Deiodinase in a Chondrocyte Cell Line.

Applications

Unspecified application

Species

Unspecified reactive species

Mana Mitsutani,Mei Yokoyama,Hiromi Hano,Midori Matsushita,Misa Hayashi,Ichiro Yamauchi,Tetsuya Tagami,Kenji Moriyama

Biology of sex differences 15:53 PubMed38987854

2024

MicroRNA-21 modulates brown adipose tissue adipogenesis and thermogenesis in a mouse model of polycystic ovary syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Samar Rezq,Alexandra M Huffman,Jelina Basnet,Amira E Alsemeh,Jussara M do Carmo,Licy L Yanes Cardozo,Damian G Romero

Cells 13: PubMed38667324

2024

The Role of -Adrenergic Receptors in Cold-Induced Beige Adipocyte Production in Pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Yang,Hong Ma,Liang Wang,Fang Wang,Jiqiao Xia,Dongyu Liu,Linlin Mu,Xiuqin Yang,Di Liu

Reproductive biology and endocrinology : RB&E 21:108 PubMed37968664

2023

Thyroid hormone transport and metabolism are disturbed in the placental villi of miscarriage.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Yu,Xinghao Feng,Zhongshan Lin,Xuan Li,Shiyue Su,Huiru Cheng,Yuanyuan Yang,Zhaolian Wei

Cell cycle (Georgetown, Tex.) 22:967-985 PubMed36710409

2023

Deficiency of lipopolysaccharide binding protein facilitates adipose browning, glucose uptake and oxygen consumption in mouse embryonic fibroblasts via activating PI3K/Akt/mTOR pathway and inhibiting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xueyao Yin,Zhiye Xu,Xinxin Zhang,Jiahua Wu,Weina Lu

Molecular metabolism :101173 PubMed33516944

2021

TFEB-deficiency attenuates mitochondrial degradation upon brown adipose tissue whitening at thermoneutrality.

Applications

Unspecified application

Species

Unspecified reactive species

Frederike Sass,Christian Schlein,Michelle Y Jaeckstein,Paul Pertzborn,Michaela Schweizer,Thorsten Schinke,Andrea Ballabio,Ludger Scheja,Joerg Heeren,Alexander W Fischer

International journal of molecular sciences 22: PubMed33374300

2020

Interplay between Thyroid Hormones and Stearoyl-CoA Desaturase 1 in the Regulation of Lipid Metabolism in the Heart.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Olichwier,Volodymyr V Balatskyi,Marcin Wolosiewicz,James M Ntambi,Pawel Dobrzyn

Molecular medicine reports 22:4289-4297 PubMed33000192

2020

Pinealectomy increases thermogenesis and decreases lipogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Mikyung Kim,So Min Lee,Jeeyoun Jung,Yun Jin Kim,Kyo Chul Moon,Ji Hae Seo,Tae Kyung Ha,Eunyoung Ha

Journal of lipid research : PubMed32769145

2020

Brown adipose tissue lipoprotein and glucose disposal is not determined by thermogenesis in UCP1- deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander W Fischer,Janina Behrens,Frederike Sass,Christian Schlein,Markus Heine,Paul Pertzborn,Ludger Scheja,Joerg Heeren

Cell reports 31:107598 PubMed32375048

2020

Control of Adipocyte Thermogenesis and Lipogenesis through β3-Adrenergic and Thyroid Hormone Signal Integration.

Applications

Unspecified application

Species

Unspecified reactive species

Adilson Guilherme,Batuhan Yenilmez,Alexander H Bedard,Felipe Henriques,Dianxin Liu,Alexandra Lee,Lauren Goldstein,Mark Kelly,Sarah M Nicoloro,Min Chen,Lee Weinstein,Sheila Collins,Michael P Czech

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DIO2 antibody

Western blot - Anti-DIO2 antibody (AB77779)

Western blot - Anti-DIO2 antibody (AB77779)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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