Rabbit Polyclonal Dio3 antibody. Suitable for WB, IHC-P and reacts with Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Rat Dio3 aa 1-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Rat Dio3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P49897
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Predicted | Predicted |
| Mouse | Predicted | Predicted |
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.2-2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 5-20 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.
Alternative names
Itdi3, Txdi3, Dio3, Thyroxine 5-deiodinase, 5DIII, DIOIII, Type 3 DI, Type III iodothyronine deiodinase
Recommended products
Rabbit Polyclonal Dio3 antibody. Suitable for WB, IHC-P and reacts with Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Rat Dio3 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Rat Dio3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P49897
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dio3 antibody (ab233035)
Formalin-fixed, paraffin-embedded rat skeletal muscle tissue stained for Dio3 using ab233035 at 20 μg/mL in immunohistochemical analysis. DAB staining.
Western blot - Anti-Dio3 antibody (ab233035)
All lanes: Western blot - Anti-Dio3 antibody (ab233035) at 1 µg/mL
All lanes: Rat liver lysate
Predicted band size: 31 kDa
Western blot - Anti-Dio3 antibody (ab233035)
All lanes: Western blot - Anti-Dio3 antibody (ab233035) at 1 µg/mL
All lanes: Rat uterus lysate
Predicted band size: 31 kDa
Western blot - Anti-Dio3 antibody (ab233035)
All lanes: Western blot - Anti-Dio3 antibody (ab233035) at 1 µg/mL
All lanes: Rat placenta lysate
Predicted band size: 31 kDa
Western blot - Anti-Dio3 antibody (ab233035)
All lanes: Western blot - Anti-Dio3 antibody (ab233035) at 1 µg/mL
All lanes: Rat blood cell lysate
Predicted band size: 31 kDa
Western blot - Anti-Dio3 antibody (ab233035)
All lanes: Western blot - Anti-Dio3 antibody (ab233035) at 1 µg/mL
All lanes: Recombinant rat Dio3 protein
Predicted band size: 31 kDa
Downloads
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