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AB233035

Anti-Dio3 antibody

3

(1 Review)

|

(1 Publication)

Rabbit Polyclonal Dio3 antibody. Suitable for WB, IHC-P and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Rat Dio3 aa 1-200.

View Alternative Names

Itdi3, Txdi3, Dio3, Thyroxine 5-deiodinase, 5DIII, DIOIII, Type 3 DI, Type III iodothyronine deiodinase

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dio3 antibody (AB233035)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dio3 antibody (AB233035)

Formalin-fixed, paraffin-embedded rat skeletal muscle tissue stained for Dio3 using ab233035 at 20 μg/mL in immunohistochemical analysis. DAB staining.

Western blot - Anti-Dio3 antibody (AB233035)
  • WB

Supplier Data

Western blot - Anti-Dio3 antibody (AB233035)

All lanes:

Western blot - Anti-Dio3 antibody (ab233035) at 1 µg/mL

All lanes:

Rat placenta lysate

Predicted band size: 31 kDa

false

Western blot - Anti-Dio3 antibody (AB233035)
  • WB

Supplier Data

Western blot - Anti-Dio3 antibody (AB233035)

All lanes:

Western blot - Anti-Dio3 antibody (ab233035) at 1 µg/mL

All lanes:

Rat blood cell lysate

Predicted band size: 31 kDa

false

Western blot - Anti-Dio3 antibody (AB233035)
  • WB

Supplier Data

Western blot - Anti-Dio3 antibody (AB233035)

All lanes:

Western blot - Anti-Dio3 antibody (ab233035) at 1 µg/mL

All lanes:

Rat uterus lysate

Predicted band size: 31 kDa

false

Western blot - Anti-Dio3 antibody (AB233035)
  • WB

Supplier Data

Western blot - Anti-Dio3 antibody (AB233035)

All lanes:

Western blot - Anti-Dio3 antibody (ab233035) at 1 µg/mL

All lanes:

Recombinant rat Dio3 protein

Predicted band size: 31 kDa

false

Western blot - Anti-Dio3 antibody (AB233035)
  • WB

Supplier Data

Western blot - Anti-Dio3 antibody (AB233035)

All lanes:

Western blot - Anti-Dio3 antibody (ab233035) at 1 µg/mL

All lanes:

Rat liver lysate

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Rat Dio3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P49897

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a crucial role in the metabolism of thyroid hormones (TH) and has specific roles in TH activation and inactivation by deiodination. Catalyzes the deiodination of L-thyroxine (T4) to 3,3',5'-triiodothyronine (rT3), 3,5-diiodothyronine (3,5-T2) to 3-monoiodothyronine (3-T1), rT3 to 3',5'-diiodothyronine (3',5'-T2) and 3,3'-diiodothyronine (3,3'-T2) to 3'-monoiodothyronine (3'-T1) via inner-ring deiodination (IRD) (By similarity). Catalyzes the deiodination of 3,5,3'-triiodothyronine (T3) to 3,3'-diiodothyronine (3,3'-T2) via IRD (PubMed : 7622463). Catalyzes the deiodination of 3-T1 to L-thyronine (T0) via outer-ring deiodination (ORD) (By similarity). Catalyzes the tyrosyl ring deiodinations of T4AM (3,3',5,5'-tetraiodothyronamine), rT3AM (3,3',5'-triiodothyronamine), T3AM (3,5,3'-triiodothyronamine), 3,5-T2AM (3,5-diiodothyronamine), 3,3'-T2AM (3,3'-diiodothyronamine) and 3-T1AM (3-iodothyronamine) (By similarity).
See full target information Dio3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:6845 PubMed34824246

2021

Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ting Chen,Qi-Yuan Yang,Yun Hu,Xiang-Dong Liu,Jeanene M de Avila,Mei-Jun Zhu,Peter W Nathanielsz,Min Du
View all publications
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Product promise

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