Anti-Diphtheria Toxin antibody (ab151222) is a rabbit polyclonal antibody that is used to detect Diphtheria Toxin in Western Blot, IP, ELISA, Dot Blot. Suitable for Corynebacterium diphtheriae samples.
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- Nature protocols 18:2374-23982023Rapid biosynthesis of glycoprotein therapeutics and vaccines from freeze-dried bacterial cell lysates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica C Stark,Thapakorn Jaroentomeechai,Katherine F Warfel,Jasmine M Hershewe,Matthew P DeLisa,Michael C JewettPubMed 37328605
- Applied microbiology and biotechnology 105:1683-16922021The recombinant protein combined vaccine based on the fragment C of tetanus toxin and the cross-reacting material 197.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengdi Chai,Xiuying Pu,Jun Ge,Sulin Ren,Xiaoyu Xia,Amiao Luo,Shiwei Wang,Xiaodong Wang,Jianqiang LiPubMed 33511443
- Proceedings of the National Academy of Sciences of 117:22090-221002020Intein-mediated cytoplasmic reconstitution of a split toxin enables selective cell ablation in mixed populations and tumor xenografts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVedud Purde et. al.PubMed 32839344
- Bioscience reports 38:2018Structural and immunological characterization of derived recombinant CRM protein used as carrier in conjugate vaccines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRavi P N Mishra et. al.PubMed 29875175
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Constituents: 100% Whole serum- Form
- Liquid
- Clonality
- Polyclonal
Reactivity data
Select an application| WB | ELISA | Dot | IP | |
|---|---|---|---|---|
| Corynebacterium diphtheriae | Tested | Expected | Expected | Expected |
| Purified native protein - Corynebacterium diphtheriae | Tested | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Corynebacterium diphtheriae | Dilution info 1/2000 | Notes Can detect 1 ng or less of the toxin |
Species Purified native protein - Corynebacterium diphtheriae | Dilution info 1/2000 | Notes Can detect 1 ng or less of the toxin |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Corynebacterium diphtheriae | Dilution info 1/2000.00000 - 1/7000.00000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein - Corynebacterium diphtheriae | Dilution info - | Notes - |
Dot
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Corynebacterium diphtheriae | Dilution info 1/1000 | Notes Can detect 1 ng or less of the toxin |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein - Corynebacterium diphtheriae | Dilution info - | Notes - |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Corynebacterium diphtheriae | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein - Corynebacterium diphtheriae | Dilution info - | Notes - |
Associated Products
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Target data
Alternative names
Corynebacterium diphtheriae toxoid
Recommended products
Anti-Diphtheria Toxin antibody (ab151222) is a rabbit polyclonal antibody that is used to detect Diphtheria Toxin in Western Blot, IP, ELISA, Dot Blot. Suitable for Corynebacterium diphtheriae samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Constituents: 100% Whole serum- Form
- Liquid
- Clonality
- Polyclonal
- Purity
- Whole antiserum
- Specificity
Reacts with Diphtheria Toxin as well as its CRM197 mutant toxin.
- Concentration
- Purification notes
Whole antiserum sterilized by filtration.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Diphtheria Toxin antibody (ab151222) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), ELISA, Dot Blot in Corynebacterium diphtheriae samples.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
Western blot - Anti-Diphtheria Toxin antibody (ab151222)
All lanes: Western blot - Anti-Diphtheria Toxin antibody (ab151222) at 1/2000 dilution
Lane 1: Crude culture medium of Corynebacterium Diphtheria.
Lane 2: The two-fold diluted culture medium.
Lane 3: Purified CRM197 (10ng)
Lane 4: Purified Diphtheria toxin (200ng)
Predicted band size: 58 kDa
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