JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB151222

Anti-Diphtheria Toxin antibody

Be the first to review this product! Submit a review

|

(5 Publications)

Anti-Diphtheria Toxin antibody (ab151222) is a rabbit polyclonal antibody detecting Diphtheria Toxin in Western Blot, IP, ELISA, Dot Blot. Suitable for Corynebacterium diphtheriae.
1 Images
Western blot - Anti-Diphtheria Toxin antibody (AB151222)
  • WB

Unknown

Western blot - Anti-Diphtheria Toxin antibody (AB151222)

All lanes:

Western blot - Anti-Diphtheria Toxin antibody (ab151222) at 1/2000 dilution

Lane 1:

Crude culture medium of Corynebacterium Diphtheria.

Lane 2:

The two-fold diluted culture medium.

Lane 3:

Purified CRM197 (10ng)

Lane 4:

Purified Diphtheria toxin (200ng)

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Corynebacterium diphtheriae

Applications

WB, Dot, IP, ELISA

applications

Specificity

Reacts with Diphtheria Toxin as well as its CRM197 mutant toxin.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Corynebacterium diphtheriae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p>Can detect 1 ng or less of the toxin</p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/2000 - 1/7000", "ELISA-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p>Can detect 1 ng or less of the toxin</p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/200 - 1/1000", "IP-species-notes": "<p></p>" }, "Purified native protein - Corynebacterium diphtheriae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p>Can detect 1 ng or less of the toxin</p>", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

Product details

What is this antibody validated in?
Anti-Diphtheria Toxin antibody (ab151222) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), ELISA, Dot Blot in Corynebacterium diphtheriae samples.

Properties and storage information

Form
Liquid
Purity
Whole antiserum
Purification notes
Whole antiserum sterilized by filtration.
Storage buffer
pH: 6 - 8.5 Constituents: 100% Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature protocols 18:2374-2398 PubMed37328605

2023

Rapid biosynthesis of glycoprotein therapeutics and vaccines from freeze-dried bacterial cell lysates.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica C Stark,Thapakorn Jaroentomeechai,Katherine F Warfel,Jasmine M Hershewe,Matthew P DeLisa,Michael C Jewett

ACS central science 9:787-804 PubMed37122450

2023

Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Tyler D Moeller,Shivem B Shah,Kristine Lai,Natalia Lopez-Barbosa,Primit Desai,Weiyao Wang,Zhe Zhong,David Redmond,Ankur Singh,Matthew P DeLisa

Applied microbiology and biotechnology 105:1683-1692 PubMed33511443

2021

The recombinant protein combined vaccine based on the fragment C of tetanus toxin and the cross-reacting material 197.

Applications

Unspecified application

Species

Unspecified reactive species

Pengdi Chai,Xiuying Pu,Jun Ge,Sulin Ren,Xiaoyu Xia,Amiao Luo,Shiwei Wang,Xiaodong Wang,Jianqiang Li

Proceedings of the National Academy of Sciences of 117:22090-22100 PubMed32839344

2020

Intein-mediated cytoplasmic reconstitution of a split toxin enables selective cell ablation in mixed populations and tumor xenografts.

Applications

Unspecified application

Species

Unspecified reactive species

Vedud Purde,Elena Kudryashova,David B Heisler,Reena Shakya,Dmitri S Kudryashov

Bioscience reports 38: PubMed29875175

2018

Structural and immunological characterization of derived recombinant CRM protein used as carrier in conjugate vaccines.

Applications

Unspecified application

Species

Unspecified reactive species

Ravi P N Mishra,Ravi S P Yadav,Christopher Jones,Salvatore Nocadello,George Minasov,Ludmilla A Shuvalova,Wayne F Anderson,Akshay Goel
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com