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AB209329

Anti-Diphtheria Toxin antibody [IGX3492]

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(1 Publication)

Human Recombinant Monoclonal Diphtheria Toxin antibody. Suitable for WB and reacts with Corynebacterium diphtheriae samples. Cited in 1 publication.

View Alternative Names

Corynebacterium diphtheriae toxoid

1 Images
Western blot - Anti-Diphtheria Toxin antibody [IGX3492] (AB209329)
  • WB

Lab

Western blot - Anti-Diphtheria Toxin antibody [IGX3492] (AB209329)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209329 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-Diphtheria Toxin antibody [IGX3492] (ab209329) at 1 µg/mL

All lanes:

Western blot - Native Diphtheria Toxin (mutated G52E) protein (<a href='/en-us/products/proteins-peptides/native-diphtheria-toxin-mutated-g52e-protein-ab188505'>ab188505</a>) at 0.1 µg

Secondary

All lanes:

HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution

Predicted band size: 58 kDa

Observed band size: 60 kDa

true

Exposure time: 5s

Key facts

Host species

Human

Clonality

Monoclonal

Clone number

IGX3492

Isotype

IgG1

Carrier free

No

Reacts with

Corynebacterium diphtheriae

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Corynebacterium diphtheriae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

Product details

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant antibody.
Human monoclonal antibody.

For recommended secondary antibodies -

Rabbit monoclonal Anti-Human IgG1 H&L (Alexa Fluor® 488) - ab200622
Rabbit monoclonal Anti-Human IgG1 H&L (Alexa Fluor® 647) - ab200623
Rabbit monoclonal Anti-Human IgG1 H&L (Biotin) - ab201485
Rabbit Anti-Human IgG H&L (HRP) - ab6759

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:22398 PubMed39333683

2024

Modulation of αv integrins by lebecetin, a viper venom-derived molecule, in experimental neuroinflammation and demyelination models.

Applications

Unspecified application

Species

Unspecified reactive species

Nour-Elhouda Neili,Zaineb AbdelKafi-Koubaa,Jed Jebali,Khouloud Kaidi,Ghada Sahraoui,Melika Ben Ahmed,Najet Srairi-Abid,Naziha Marrakchi,Raoudha Doghri,Ines ELBini
View all publications

Product promise

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