AB219834

Anti-Diphtheria Toxin antibody [IGX3492R-3]

Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Diphtheria Toxin antibody. Suitable for WB and reacts with Corynebacterium diphtheriae samples. Cited in 1 publication.

View Alternative Names

Corynebacterium diphtheriae toxoid

1 Images

Lab

Western blot - Anti-Diphtheria Toxin antibody [IGX3492R-3] (AB219834)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab219834 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-Diphtheria Toxin antibody [IGX3492R-3] (ab219834) at 0.5 µg/mL

All lanes:

Western blot - Native Diphtheria Toxin (mutated G52E) protein (<a href='/en-us/products/proteins-peptides/native-diphtheria-toxin-mutated-g52e-protein-ab188505'>ab188505</a>) at 0.1 µg

Secondary

All lanes:

Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 58 kDa

Observed band size: 60 kDa

true

Exposure time: 5s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

IGX3492R-3

Isotype

IgG1

Carrier free

Reacts with

Corynebacterium diphtheriae

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Corynebacterium diphtheriae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Diphtheria Toxin

Additional targets

Diphtheria Toxin

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 10: PubMed34259627

2021

CEP78 functions downstream of CEP350 to control biogenesis of primary cilia by negatively regulating CP110 levels.

Applications

Unspecified application

Species

Unspecified reactive species

André Brás Gonçalves,Sarah Kirstine Hasselbalch,Beinta Biskopstø Joensen,Sebastian Patzke,Pernille Martens,Signe Krogh Ohlsen,Mathieu Quinodoz,Konstantinos Nikopoulos,Reem Suleiman,Magnus Per Damsø Jeppesen,Catja Weiss,Søren Tvorup Christensen,Carlo Rivolta,Jens S Andersen,Pietro Farinelli,Lotte Bang Pedersen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Diphtheria Toxin antibody [IGX3492R-3]

Western blot - Anti-Diphtheria Toxin antibody [IGX3492R-3] (AB219834)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (1)

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Product promise