Rabbit Polyclonal DIRAS1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DIRAS1.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human DIRAS1. Database link O95057
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Displays low GTPase activity and exists predominantly in the GTP-bound form.
Alternative names
GBTS1, RIG, DIRAS1, GTP-binding protein Di-Ras1, Distinct subgroup of the Ras family member 1, Ras-related inhibitor of cell growth, Small GTP-binding tumor suppressor 1, Rig
Recommended products
Rabbit Polyclonal DIRAS1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DIRAS1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human DIRAS1. Database link O95057
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DIRAS1 also known as DIRAS family GTPase 1 is a small GTPase with a molecular weight of approximately 22 kDa. It is a member of the Ras superfamily known for its role in cell signaling and growth regulation. DIRAS1 is ubiquitously expressed in various tissues with notable expression in the brain heart and kidney suggesting its involvement in essential cellular processes. Structurally DIRAS1 is similar to other Ras-like GTPases comprising a highly conserved G-domain which facilitates its function in GTP binding and hydrolysis.
- Biological function summary
DIRAS1 is involved in cellular proliferation and migration playing a significant role in maintaining cellular architecture and signal transduction. DIRAS1 is not typically part of a large protein complex but interacts with various effector proteins that modulate its activity. Its function in controlling cell growth suggests that it may act as a tumor suppressor in certain tissues. Furthermore its interaction with the cytoskeleton highlights its importance in cellular movement and organization.
- Pathways
DIRAS1 takes part in significant cellular signaling pathways including the MAPK/ERK and PI3K/AKT pathways. These pathways are vital for cell growth survival and differentiation. DIRAS1 modulates these pathways by interacting with key proteins such as RAF1 and Akt influencing their downstream signaling. By regulating these pathways DIRAS1 contributes to a balance between cellular proliferation and apoptosis thereby playing a critical role in maintaining cellular homeostasis.
- Associated diseases and disorders
Alterations in DIRAS1 expression or function have associations with various cancers including glioblastoma and colorectal cancer. DIRAS1's potential tumor-suppressive function suggests that its downregulation may contribute to tumorigenesis. The loss of its regulatory control on the MAPK/ERK pathway results in unchecked cellular proliferation. Additionally in glioblastoma DIRAS1 may interact with altered p53 protein levels highlighting a link between DIRAS1 dysfunction and cancerous growth. Researchers are exploring DIRAS1's potential as a target for therapeutic intervention given its prominent role in disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIRAS1 antibody (ab238491)
Paraffin-embedded human brain tissue stained for DIRAS1 using ab238491 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunocytochemistry/ Immunofluorescence - Anti-DIRAS1 antibody (ab238491)
A549 (Human lung carcinoma cell line) cells stained for DIRAS1 (green) using ab238491 at 1/66 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.
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