Rabbit Polyclonal DIRAS1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DIRAS1.
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GBTS1, RIG, DIRAS1, GTP-binding protein Di-Ras1, Distinct subgroup of the Ras family member 1, Ras-related inhibitor of cell growth, Small GTP-binding tumor suppressor 1, Rig
- ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-DIRAS1 antibody (AB238491)
A549 (Human lung carcinoma cell line) cells stained for DIRAS1 (green) using ab238491 at 1/66 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIRAS1 antibody (AB238491)
Paraffin-embedded human brain tissue stained for DIRAS1 using ab238491 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Supplementary information
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Biological function summary
DIRAS1 is involved in cellular proliferation and migration playing a significant role in maintaining cellular architecture and signal transduction. DIRAS1 is not typically part of a large protein complex but interacts with various effector proteins that modulate its activity. Its function in controlling cell growth suggests that it may act as a tumor suppressor in certain tissues. Furthermore its interaction with the cytoskeleton highlights its importance in cellular movement and organization.
Pathways
DIRAS1 takes part in significant cellular signaling pathways including the MAPK/ERK and PI3K/AKT pathways. These pathways are vital for cell growth survival and differentiation. DIRAS1 modulates these pathways by interacting with key proteins such as RAF1 and Akt influencing their downstream signaling. By regulating these pathways DIRAS1 contributes to a balance between cellular proliferation and apoptosis thereby playing a critical role in maintaining cellular homeostasis.
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