AB70245

Anti-DIS antibody

Name: Anti-DIS antibody (ab70245) | Abcam
Brand: Abcam Limited
SKU: AB70245
Price: 495 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal DIS antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CCAR1.

View Alternative Names

CARP1, DIS, CCAR1, Cell division cycle and apoptosis regulator protein 1, Cell cycle and apoptosis regulatory protein 1, Death inducer with SAP domain, CARP-1

2 Images

Unknown

Immunoprecipitation - Anti-DIS antibody (AB70245)

Detection of Human CCAR1 by Immunoprecipitation using Nuclear extract (100 μg) from HeLa cells and Affinity purified rabbit anti-CCAR1 ab70245 at 1 μg/100 μg Nuclear extract. Chemiluminescence with exposure times of approximately 3 seconds.

All lanes:

Immunoprecipitation - Anti-DIS antibody (ab70245)

Predicted band size: 133 kDa

false

Unknown

Western blot - Anti-DIS antibody (AB70245)

Lanes 1 - 2:

Western blot - Anti-DIS antibody (ab70245) at 1 µg/mL

Lanes 3 - 4:

Western blot - Anti-DIS antibody (ab70245) at 0.2 µg/mL

Lanes 1 and 3:

Nuclear extract from HeLa cells at 15 µg

Lanes 2 and 4:

Nuclear extract from HeLa cells at 45 µg

Predicted band size: 133 kDa

Observed band size: 132 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human CCAR1. The exact immunogen used to generate this antibody is proprietary information.

Q8IX12

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibody was affinity purified using an epitope specific to DIS immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DIS also known as the Death Inducing Signaling Complex functions as a mechanical trigger for apoptosis. A multi-protein complex the mass of DIS varies as it consists of several proteins assembling together. Located primarily in the cytoplasm it plays a significant role in cellular death programs. The expression of its components occurs widely but especially in cells undergoing stress or programmed cell death.

Biological function summary

Several proteins converge within the Death Inducing Signaling Complex to initiate apoptotic pathways. The complex includes proteins such as procaspases and adapter proteins like FADD. DIS's primary role is to recruit and activate procaspase-8 which then instigates a cascade of events leading to cell apoptosis. This process is important for maintaining cellular health and removing damaged or dangerous cells within organisms.

Pathways

DIS serves as a central hub in the extrinsic apoptotic pathway. Key proteins such as Fas receptor (CD95) and TNF receptor contribute to its activation. These receptors when bound to their ligands provide the initial step for DIS formation beginning the apoptosis signaling cascade. The involvement of DIS in the extrinsic pathway showcases its importance in controlling and regulating apoptosis efficiently.

Aberrations in the Death Inducing Signaling Complex function associate with chronic conditions and cancer. Overactive DIS signaling can contribute to excessive cell death leading to neurodegenerative diseases. Conversely impaired DIS activity may result in inadequate apoptosis allowing cancerous cells to survive longer. In these disorders interactions with proteins like FLIP are significant as it can inhibit DIS function altering the apoptosis process and impacting disease progression.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation (PubMed : 12816952). In association with CALCOCO1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed : 24245781). Can act as a both a coactivator and corepressor of AR-mediated transcription. Contributes to chromatin looping and AR transcription complex assembly by stabilizing AR-GATA2 association on chromatin and facilitating MED1 and RNA polymerase II recruitment to AR-binding sites. May play an important role in the growth and tumorigenesis of prostate cancer cells (PubMed : 23887938).

See full target information CCAR1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular pharmacology 95:120-126 PubMed30397001

2018

Cell Cycle and Apoptosis Regulator 1, CCAR1, Regulates Enhancer-Dependent Nuclear Receptor CAR Transactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuichiro Kanno,Shuai Zhao,Naoya Yamashita,Nao Saito,Aoi Ujiie,Rie Iijima,Nami Kikawa,Kiyomitsu Nemoto,Yoshio Inouye

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DIS antibody

Immunoprecipitation - Anti-DIS antibody (AB70245)

Western blot - Anti-DIS antibody (AB70245)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise