Rabbit Polyclonal DIS3 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human DIS3 aa 1-50.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human DIS3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y2L1
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-10 µg | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan, Rat, Rabbit, Horse, Cow, Cynomolgus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan, Rat, Rabbit, Horse, Cow, Cynomolgus monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.
Alternative names
Exosome complex exonuclease RRP44, Protein DIS3 homolog, Ribosomal RNA-processing protein 44, RRP44, KIAA1008, DIS3
Recommended products
Rabbit Polyclonal DIS3 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human DIS3 aa 1-50.
Alternative names
Exosome complex exonuclease RRP44, Protein DIS3 homolog, Ribosomal RNA-processing protein 44, RRP44, KIAA1008, DIS3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human DIS3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y2L1
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab226186 was affinity purified using an epitope specific to DIS3 immobilized on solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
DIS3 also known as RRP44 is an exoribonuclease and an essential component of the RNA degradation machinery. It possesses a molecular mass of approximately 110 kDa and functions as an RNA helicase and 3'-5' exoribonuclease. DIS3 is expressed in various tissues including the bone marrow and lymphoid tissues. The protein acts to degrade defective RNA molecules and regulate RNA levels ensuring accurate gene expression and proper cellular function.
- Biological function summary
DIS3 participates in the RNA exosome complex a multi-protein complex responsible for processing and degrading RNA. This complex processes a wide range of RNA substrates including pre-rRNA snRNA and snoRNA. DIS3 performs its function by catalyzing the exonucleolytic cleavage of these RNA molecules regulating their abundance and contributing to RNA quality control. As a critical member of the exosome DIS3 is essential for cellular RNA homeostasis and plays a role in maintaining the stability of the transcriptome.
- Pathways
RNA metabolism and degradation significantly involve DIS3 impacting mRNA surveillance and decay pathways. It notably contributes to the Nonsense-Mediated mRNA Decay (NMD) pathway which detects and degrades mRNAs containing premature stop codons. DIS3 interacts with other exosome components such as RRP6 and MTR4 facilitating the processing of diverse RNA species. The protein’s role is integral to overall RNA turnover and quality control ensuring effective removal of defective RNA transcripts and preventing inappropriate protein synthesis.
- Associated diseases and disorders
Mutations or dysregulation of DIS3 relate to multiple myeloma and acute myeloid leukemia. In these conditions aberrant RNA degradation may contribute to the pathogenesis by altering normal RNA processing and expression profiles. DIS3 mutations can lead to impaired function of the exosome enhancing the survival and proliferation of malignant cells. Additionally proteins such as RRP6 are connected with DIS3 through their roles in the exosome machinery suggesting combined involvement in RNA processing-related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-DIS3 antibody (ab226186)
All lanes: Western blot - Anti-DIS3 antibody (ab226186) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 109 kDa
Exposure time: 3min
Immunoprecipitation - Anti-DIS3 antibody (ab226186)
DIS3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226186 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226186 at 1 μg/ml.
Lane 1: ab226186 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-DIS3 antibody (ab226186)
Predicted band size: 109 kDa
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