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AB181743

Anti-DIS3L2 antibody [6C7B2]

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(1 Publication)

Mouse Monoclonal DIS3L2 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DIS3L2 aa 1-250.

View Alternative Names

FAM6A, DIS3L2, DIS3-like exonuclease 2, hDIS3L2

6 Images
Flow Cytometry - Anti-DIS3L2 antibody [6C7B2] (AB181743)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-DIS3L2 antibody [6C7B2] (AB181743)

Flow Cytometrical analysis of Jurkat cells labeling DIS3L2 with ab181743 at 1/200 (green) compared to a negative control antibody (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIS3L2 antibody [6C7B2] (AB181743)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIS3L2 antibody [6C7B2] (AB181743)

Immunohistochemical analysis of paraffin embedded Human endometrial cancer tissue labeling DIS3L2 with ab181743 at 1/200.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIS3L2 antibody [6C7B2] (AB181743)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIS3L2 antibody [6C7B2] (AB181743)

Immunohistochemical analysis of paraffin embedded Human bladder cancer tissue labeling DIS3L2 with ab181743 at 1/200.

Western blot - Anti-DIS3L2 antibody [6C7B2] (AB181743)
  • WB

Supplier Data

Western blot - Anti-DIS3L2 antibody [6C7B2] (AB181743)

All lanes:

Western blot - Anti-DIS3L2 antibody [6C7B2] (ab181743) at 1/500 dilution

Lane 1:

HeLa cell lysate

Lane 2:

HepG2 cell lysate

Predicted band size: 99 kDa

false

Western blot - Anti-DIS3L2 antibody [6C7B2] (AB181743)
  • WB

Supplier Data

Western blot - Anti-DIS3L2 antibody [6C7B2] (AB181743)

All lanes:

Western blot - Anti-DIS3L2 antibody [6C7B2] (ab181743) at 1/500 dilution

All lanes:

DIS3L2 recombinant protein

Predicted band size: 27 kDa,68 kDa,99 kDa

false

Western blot - Anti-DIS3L2 antibody [6C7B2] (AB181743)
  • WB

Supplier Data

Western blot - Anti-DIS3L2 antibody [6C7B2] (AB181743)

All lanes:

Western blot - Anti-DIS3L2 antibody [6C7B2] (ab181743) at 1/500 dilution

Lane 1:

non-transfected HEK293 cell lysate

Lane 2:

DIS3L2 (aa 27-250)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 99 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6C7B2

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DIS3L2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q8IYB7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DIS3L2 also known as DIS3-like exonuclease 2 is a protein involved in RNA processing and degradation. Mechanically it functions as a 3'-5' exonuclease meaning it removes nucleotides one by one from the 3' end of RNA molecules. The protein has a molecular mass of approximately 110 kDa. It is mainly expressed in the cytoplasm where it plays a critical role in maintaining RNA stability and turnover. DIS3L2 is present in various tissues with notable expression in the kidney and reproductive organs.
Biological function summary

The protein contributes to RNA metabolism by degrading small non-coding RNAs such as ribosomal RNA (rRNA) and transfer RNA (tRNA) fragments. It is not known to be part of a complex instead acting as an individual enzyme. DIS3L2 helps control the quantity and quality of RNA molecules in cells preventing accumulation of aberrant or excessive RNAs that can disrupt cellular processes.

Pathways

DIS3L2 participates in the RNA surveillance pathway ensuring proper RNA processing and maturation. This surveillance system involves several proteins that recognize and degrade abnormal RNAs therefore preserving genomic integrity. DIS3L2 collaborates with components of the exosome complex to achieve this function. In the regulatory RNAs pathway it interacts with proteins that manage microRNA decay thereby influencing gene expression by modulating microRNA levels.

Dysfunctional activity of DIS3L2 has been linked to Perlman syndrome a condition characterized by overgrowth and predisposition to Wilms tumor a type of kidney cancer. Studies suggest that DIS3L2 through its RNA degradation role impacts the balance of growth-regulatory factors. Additionally it has been associated with Bloom syndrome a disorder marked by genomic instability. Here the absence or malfunction of DIS3L2 can exacerbate the genomic instability that characterizes Bloom syndrome highlighting its interaction with pathways involving DNA repair proteins.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of both mRNAs and miRNAs that have been polyuridylated by a terminal uridylyltransferase, such as ZCCHC11/TUT4. Mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'. Mediates degradation of uridylated pre-let-7 miRNAs, contributing to the maintenance of embryonic stem (ES) cells. Essential for correct mitosis, and negatively regulates cell proliferation.
See full target information DIS3L2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 6:24728 PubMed27090737

2016

Knockdown of lncRNA-ATB suppresses autocrine secretion of TGF-β2 by targeting ZNF217 via miR-200c in keloid fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Hua-Yu Zhu,Wen-Dong Bai,Chao Li,Zhao Zheng,Hao Guan,Jia-Qi Liu,Xue-Kang Yang,Shi-Chao Han,Jian-Xin Gao,Hong-Tao Wang,Da-Hai Hu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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