AB124933

Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145]

RabMAb
Recombinant
What is this?

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(13 Publications)

Rabbit Recombinant Monoclonal Dishevelled 2 phospho S143 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 13 publications.

View Alternative Names

Segment polarity protein dishevelled homolog DVL-2, Dishevelled-2, DSH homolog 2, DVL2

3 Images

Lab

Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (AB124933)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/5000 dilution

Lane 1:

Untreated HEK293 cell lysate at 10 µg

Lane 2:

Alkaline phosphatase treated HEK293 cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 78 kDa

Observed band size: 95 kDa

false

Lab

Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (AB124933)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/1000 dilution

Lane 1:

Untreated F9 cell lysate at 10 µg

Lane 2:

Alkaline phosphatase treated F9 cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 78 kDa

Observed band size: 95 kDa

false

Unknown

Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (AB124933)

All lanes:

Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/1000 dilution

Lane 1:

F9 cell lysate untreated at 10 µg

Lane 2:

F9 cell lysate treated with Alkaline Phosphatase at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 78 kDa

Observed band size: 95 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPNCIR145

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/1000 - 1/10000.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/1000 - 1/10000.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Kyung Lee. View antibodies from NCI Center for Cancer Research Collaboration.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in the signal transduction pathways mediated by multiple Wnt genes (PubMed : 24616100). Participates both in canonical and non-canonical Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling.

See full target information DVL2 phospho S143

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 43:255-272 PubMed39386219

2024

Self-assembled PROTACs enable protein degradation to reprogram the tumor microenvironment for synergistically enhanced colorectal cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Xinchen Lu,Jinmei Jin,Ye Wu,Jiayi Lin,Xiaokun Zhang,Shengxin Lu,Jiyuan Zhang,Chunling Zhang,Maomao Ren,Hongzhuan Chen,Weidong Zhang,Xin Luan

Life science alliance 7: PubMed39366759

2024

SLMAP3 is essential for neurulation through mechanisms involving cytoskeletal elements, ABP, and PCP.

Applications

Unspecified application

Species

Unspecified reactive species

Taha Rehmani,Ana Paula Dias,Billi Dawn Applin,Maysoon Salih,Balwant S Tuana

Human molecular genetics 30:2402-2415 PubMed34274973

2021

Loss of Zic3 impairs planar cell polarity leading to abnormal left-right signaling, heart defects and neural tube defects.

Applications

Unspecified application

Species

Unspecified reactive species

Helen M Bellchambers,Stephanie M Ware

Scientific reports 10:17443 PubMed33060778

2020

Cir-ITCH inhibits gastric cancer migration, invasion and proliferation by regulating the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Peng,Hong Hong Wang

Frontiers in cell and developmental biology 8:465 PubMed32587859

2020

The Autophagy Regulator p62 Controls PTEN-Dependent Ciliogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Hyowon Mun,Eun Ji Lee,Minah Park,Goo Taeg Oh,Jong Hoon Park

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:3413-3428 PubMed31922321

2020

E2F1-Ror2 signaling mediates coordinated transcriptional regulation to promote G1/S phase transition in bFGF-stimulated NIH/3T3 fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Mitsuharu Endo,Yuki Tanaka,Mako Otsuka,Yasuhiro Minami

Oncology letters 18:3845-3856 PubMed31579410

2019

The CK2 inhibitor CX4945 reverses cisplatin resistance in the A549/DDP human lung adenocarcinoma cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Chengji Jin,Ping Song,Ji Pang

Theranostics 9:3707-3722 PubMed31281508

2019

Nerve modulation therapy in gouty arthritis: targeting increased sFRP2 expression in dorsal root ganglion regulates macrophage polarization and alleviates endothelial damage.

Applications

Unspecified application

Species

Unspecified reactive species

Jingtian Mei,Feng Zhou,Han Qiao,Hanjun Li,Tingting Tang

Nature communications 10:2356 PubMed31142743

2019

Atypical function of a centrosomal module in WNT signalling drives contextual cancer cell motility.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Luo,Miriam Barrios-Rodiles,Gagan D Gupta,Ying Y Zhang,Abiodun A Ogunjimi,Mikhail Bashkurov,Johnny M Tkach,Ainsley Q Underhill,Liang Zhang,Mohamed Bourmoum,Jeffrey L Wrana,Laurence Pelletier

Scientific reports 8:2715 PubMed29426949

2018

PP5 (PPP5C) is a phosphatase of Dvl2.

Applications

Unspecified application

Species

Unspecified reactive species

Jianlei Xie,Meng Han,Miaojun Zhang,Haiteng Deng,Wei Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145]

Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (AB124933)

Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (AB124933)

Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (AB124933)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (13)

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