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AB169950

Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Dishevelled 2 phospho S143 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

Segment polarity protein dishevelled homolog DVL-2, Dishevelled-2, DSH homolog 2, DVL2

3 Images
Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (AB169950)
  • WB

Lab

Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (AB169950)

This data was developed using ab124933, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (<a href='/en-us/products/primary-antibodies/dishevelled-2-phospho-s143-antibody-epncir145-ab124933'>ab124933</a>) at 1/5000 dilution

Lane 1:

Untreated HEK293 cell lysate at 10 µg

Lane 2:

Alkaline phosphatase treated HEK293 cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 78 kDa

Observed band size: 95 kDa

false

Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (AB169950)
  • WB

Lab

Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (AB169950)

This data was developed using ab124933, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (<a href='/en-us/products/primary-antibodies/dishevelled-2-phospho-s143-antibody-epncir145-ab124933'>ab124933</a>) at 1/1000 dilution

Lane 1:

Untreated F9 cell lysate at 10 µg

Lane 2:

Alkaline phosphatase treated F9 cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 78 kDa

Observed band size: 95 kDa

false

Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (AB169950)
  • WB

Unknown

Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (AB169950)

This data was developed using ab124933, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (<a href='/en-us/products/primary-antibodies/dishevelled-2-phospho-s143-antibody-epncir145-ab124933'>ab124933</a>) at 1/1000 dilution

Lane 1:

F9 cell lysate untreated at 10 µg

Lane 2:

F9 cell lysate treated with Alkaline Phosphatase at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 78 kDa

Observed band size: 95 kDa

false

  • Unconjugated

    Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPNCIR145

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab169950 is the carrier-free version of ab124933.

This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Kyung Lee. View antibodies from NCI Center for Cancer Research Collaboration.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the signal transduction pathways mediated by multiple Wnt genes (PubMed : 24616100). Participates both in canonical and non-canonical Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling.
See full target information DVL2 phospho S143
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 14:687-694 PubMed28693222

2017

Identification of differentially expressed inflammatory factors in Wilms tumors and their association with patient outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Guo,Junjie Zhang,Lei Wang,Wei Zhao,Jiekai Yu,Shu Zheng,Jiaxiang Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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