Rabbit Recombinant Monoclonal Dishevelled 2 phospho T224 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.
Images
![Western blot - Anti-Dishevelled 2 (phospho T224) antibody [EPNCIR146] (AB124941), expandable thumbnail](https://content.abcam.com/products/images/dishevelled-2-phospho-t224-antibody-epncir146-ab124941--western-blot-img114983.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Dishevelled 2 (phospho T224) antibody [EPNCIR146] (AB124941)")
Publications
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- Glia 72:411-4322023Wnt5a/Ror2 promotes Nrf2-mediated tissue protective function of astrocytes after brain injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMitsuharu Endo,Yuki Tanaka,Mayo Fukuoka,Hayata Suzuki,Yasuhiro MinamiPubMed 37904612
- Biochemical and biophysical research communication 532:406-4132020The Daple-CK1ε complex regulates Dvl2 phosphorylation and canonical Wnt signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNobutoshi Esaki et. al.PubMed 32888647
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:3413-34282020E2F1-Ror2 signaling mediates coordinated transcriptional regulation to promote G1/S phase transition in bFGF-stimulated NIH/3T3 fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMitsuharu Endo,Yuki Tanaka,Mako Otsuka,Yasuhiro MinamiPubMed 31922321
- Oncology letters 18:3845-38562019The CK2 inhibitor CX4945 reverses cisplatin resistance in the A549/DDP human lung adenocarcinoma cell line.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChengji Jin et. al.PubMed 31579410
- Science (New York, N.Y.) 361:2018A molecular mechanism for Wnt ligand-specific signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie Eubelen,Naguissa Bostaille,Pauline Cabochette,Anne Gauquier,Patricia Tebabi,Andra C Dumitru,Melanie Koehler,Philipp Gut,David Alsteens,Didier Y R Stainier,Abel Garcia-Pino,Benoit VanhollebekePubMed 30026314
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Predicted | Not recommended |
| Rat | Not recommended | Not recommended | Predicted | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Plays a role in the signal transduction pathways mediated by multiple Wnt genes (PubMed:24616100). Participates both in canonical and non-canonical Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling.
Alternative names
Segment polarity protein dishevelled homolog DVL-2, Dishevelled-2, DSH homolog 2, DVL2
Recommended products
Rabbit Recombinant Monoclonal Dishevelled 2 phospho T224 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPNCIR146
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Kyung Lee. View antibodies from NCI Center for Cancer Research Collaboration.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-Dishevelled 2 (phospho T224) antibody [EPNCIR146] (ab124941), expandable thumbnail](https://content.abcam.com/products/images/dishevelled-2-phospho-t224-antibody-epncir146-ab124941--western-blot-img114983.jpg "Western blot - Anti-Dishevelled 2 (phospho T224) antibody [EPNCIR146] (ab124941)")
Western blot - Anti-Dishevelled 2 (phospho T224) antibody [EPNCIR146] (ab124941)
All lanes: Western blot - Anti-Dishevelled 2 (phospho T224) antibody [EPNCIR146] (ab124941) at 1/1000 dilution
Lane 1: HeLa cell lysate untreated at 10 µg
Lane 2: HeLa cell lysate treated with Calyculin A at 10 µg
SecondaryAll lanes: HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 78 kDa
Observed band size: 95 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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