JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB76081

Anti-Dishevelled 3 antibody [EP1991Y]

4

(2 Reviews)

|

(8 Publications)

Rabbit Monoclonal Dishevelled 3 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

View Alternative Names

KIAA0208, DVL3, Segment polarity protein dishevelled homolog DVL-3, Dishevelled-3, DSH homolog 3

3 Images
Flow Cytometry (Intracellular) - Anti-Dishevelled 3 antibody [EP1991Y] (AB76081)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Dishevelled 3 antibody [EP1991Y] (AB76081)

Flow cytometry analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) labelling Dishevelled 3 with ab76081 at 1/200 dilution, followed by blocking with 10% goat serum for 1h at room temperature. ab76081 was incubated for 30 min at room temperature (RT) and then Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) was incubated for 30 min at room temperature.

Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 3 antibody [EP1991Y] (AB76081)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 3 antibody [EP1991Y] (AB76081)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 cells labelling Dishevelled 3 with ab76081 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution at RT for 45 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889) was used to counterstain tubulin at 1/200 dilution. Nucleus were visualized using DAPI.

Western blot - Anti-Dishevelled 3 antibody [EP1991Y] (AB76081)
  • WB

Lab

Western blot - Anti-Dishevelled 3 antibody [EP1991Y] (AB76081)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lane 1, 20 seconds; Lane 2 and Lane 3, 40 seconds.

Double bands of DVL3 could be observed which was supposed to be caused by phosphorylation supported by literatures (PMID : 31870452, 22647544).

All lanes:

Western blot - Anti-Dishevelled 3 antibody [EP1991Y] (ab76081) at 1/1000 dilution

Lane 1:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg

Lane 2:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 15 µg

Lane 3:

C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

  • Carrier free

    Anti-Dishevelled 3 antibody [EP1991Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1991Y

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/30", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in the signal transduction pathway mediated by multiple Wnt genes.
See full target information DVL3
style
left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:8388 PubMed35589804

2022

DVL1 and DVL3 require nuclear localisation to regulate proliferation in human myoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Johanna Pruller,Nicolas Figeac,Peter S Zammit

HGG advances 3:100111 PubMed35571680

2022

variants of cause a new intellectual disability-craniodigital syndrome by disrupting the canonical Wnt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Asif,Emrah Kaygusuz,Marwan Shinawi,Anna Nickelsen,Tzung-Chien Hsieh,Prerana Wagle,Birgit S Budde,Jennifer Hochscherf,Uzma Abdullah,Stefan Höning,Christian Nienberg,Dirk Lindenblatt,Angelika A Noegel,Janine Altmüller,Holger Thiele,Susanne Motameny,Nicole Fleischer,Idan Segal,Lynn Pais,Sigrid Tinschert,Nadra Nasser Samra,Juliann M Savatt,Natasha L Rudy,Chiara De Luca, Paola Fortugno,Susan M White,Peter Krawitz,Anna C E Hurst,Karsten Niefind,Joachim Jose,Francesco Brancati,Peter Nürnberg,Muhammad Sajid Hussain

Cellular physiology and biochemistry : internation 50:1815-1831 PubMed30396168

2018

Secreted Frizzled Related Protein 2 Modulates Epithelial-Mesenchymal Transition and Stemness via Wnt/β-Catenin Signaling in Choriocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xianling Zeng,Yafei Zhang,Huiqiu Xu,Taohong Zhang,Yan Xue,Ruifang An

The Journal of biological chemistry 293:19710-19724 PubMed30361437

2018

Oligomerization of Frizzled and LRP5/6 protein initiates intracellular signaling for the canonical WNT/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Hua,Yilin Yang,Qian Li,Xinyu He,Wei Zhu,Jiyong Wang,Xiaoqing Gan

Oncology letters 12:199-206 PubMed27347125

2016

Low expression of secreted frizzled-related protein 2 and nuclear accumulation of β-catenin in aggressive nonfunctioning pituitary adenoma.

Applications

WB

Species

Human

Youtu Wu,Jiwei Bai,Linchuan Hong,Chunhui Liu,Shengyuan Yu,Guoqiang Yu,Yazhuo Zhang

International journal of molecular medicine 35:1587-95 PubMed25847080

2015

miR-204-5p promotes the adipogenic differentiation of human adipose-derived mesenchymal stem cells by modulating DVL3 expression and suppressing Wnt/β-catenin signaling.

Applications

WB

Species

Unspecified reactive species

Honghui He,Ke Chen,Fang Wang,Liling Zhao,Xinxing Wan,Linghao Wang,Zhaohui Mo

Hepatitis monthly 13:e6192 PubMed23483081

2013

Differential Expression of Wnt Pathway Genes in Sporadic Hepatocellular Carcinomas Infected With Hepatitis B Virus Identified With OligoGE Arrays.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Lin,Qiangxiu Wang,Zhixin Cao,Ming Geng,Yongcheng Cao,Xiaohong Liu

Human molecular genetics 20:2611-27 PubMed21498478

2011

Control of the Wnt pathways by nephrocystin-4 is required for morphogenesis of the zebrafish pronephros.

Applications

IHC (PFA fixed)

Species

Unspecified reactive species

Céline Burcklé,Helori-Mael Gaudé,Christine Vesque,Flora Silbermann,Rémi Salomon,Cécile Jeanpierre,Corinne Antignac,Sophie Saunier,Sylvie Schneider-Maunoury
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB152161

Anti-Dishevelled 3 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dishevelled 3 antibody - N-terminal (AB152161)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB228804

Anti-Dishevelled 2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 2 antibody (AB228804)

  • 0
  • 0
Primary Antibodies

AB236632

Anti-HA tag antibody [EPR22819-101]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [EPR22819-101] (AB236632)

  • 3
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB32572

Anti-beta Catenin antibody [E247] - ChIP Grade

BOND RX™ Validated|Advanced Validation|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [E247] - ChIP Grade (AB32572)

  • 5
  • 75

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com