Rabbit Recombinant Monoclonal Dishevelled 3 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-Dishevelled 3 antibody [EP1991Y] - BSA and Azide free (AB284737), expandable thumbnail](https://content.abcam.com/products/images/dishevelled-3-antibody-ep1991y-bsa-and-azide-free-ab284737--western-blot-img437483.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Dishevelled 3 antibody [EP1991Y] - BSA and Azide free (AB284737)")
![Flow Cytometry (Intracellular) - Anti-Dishevelled 3 antibody [EP1991Y] - BSA and Azide free (AB284737), expandable thumbnail](https://content.abcam.com/products/images/dishevelled-3-antibody-ep1991y-bsa-and-azide-free-ab284737--flow-cytometry-intracellular-img437485.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Dishevelled 3 antibody [EP1991Y] - BSA and Azide free (AB284737)")
![Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 3 antibody [EP1991Y] - BSA and Azide free (AB284737), expandable thumbnail](https://content.abcam.com/products/images/dishevelled-3-antibody-ep1991y-bsa-and-azide-free-ab284737--immunocytochemistry-immunofluorescence-img437484.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 3 antibody [EP1991Y] - BSA and Azide free (AB284737)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Expected | Tested |
| Mouse | Not recommended | Tested | Expected | Expected |
| Rat | Not recommended | Tested | Tested | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Involved in the signal transduction pathway mediated by multiple Wnt genes.
Alternative names
KIAA0208, DVL3, Segment polarity protein dishevelled homolog DVL-3, Dishevelled-3, DSH homolog 3
Recommended products
Rabbit Recombinant Monoclonal Dishevelled 3 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP1991Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab284737 is the carrier-free version of Anti-Dishevelled 3 antibody [EP1991Y] ab76081.
This product has switched from a hybridoma to recombinant production method on 13th September 2024.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Dishevelled 3 antibody [EP1991Y] - BSA and Azide free (ab284737), expandable thumbnail](https://content.abcam.com/products/images/dishevelled-3-antibody-ep1991y-bsa-and-azide-free-ab284737--western-blot-img437483.jpg "Western blot - Anti-Dishevelled 3 antibody [EP1991Y] - BSA and Azide free (ab284737)")
Western blot - Anti-Dishevelled 3 antibody [EP1991Y] - BSA and Azide free (ab284737)
This data was developed using Anti-Dishevelled 3 antibody [EP1991Y] ab76081, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1, 20 seconds; Lane 2 and Lane 3, 40 seconds.
Double bands of DVL3 could be observed which was supposed to be caused by phosphorylation supported by literatures (PMID: 31870452, 22647544).
All lanes: Western blot - Anti-Dishevelled 3 antibody [EP1991Y] (Anti-Dishevelled 3 antibody [EP1991Y] ab76081) at 1/1000 dilution
Lane 1: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg
Lane 2: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 15 µg
Lane 3: C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
![Flow Cytometry (Intracellular) - Anti-Dishevelled 3 antibody [EP1991Y] - BSA and Azide free (ab284737), expandable thumbnail](https://content.abcam.com/products/images/dishevelled-3-antibody-ep1991y-bsa-and-azide-free-ab284737--flow-cytometry-intracellular-img437485.jpg "Flow Cytometry (Intracellular) - Anti-Dishevelled 3 antibody [EP1991Y] - BSA and Azide free (ab284737)")
Flow Cytometry (Intracellular) - Anti-Dishevelled 3 antibody [EP1991Y] - BSA and Azide free (ab284737)
This data was developed using Anti-Dishevelled 3 antibody [EP1991Y] ab76081, the same antibody clone in a different buffer formulation.
Flow cytometry analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) labelling Dishevelled 3 with Anti-Dishevelled 3 antibody [EP1991Y] ab76081 at 1/200 dilution, followed by blocking with 10% goat serum for 1h at room temperature. Anti-Dishevelled 3 antibody [EP1991Y] ab76081 was incubated for 30 min at room temperature (RT) and then Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) was incubated for 30 min at room temperature.
![Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 3 antibody [EP1991Y] - BSA and Azide free (ab284737), expandable thumbnail](https://content.abcam.com/products/images/dishevelled-3-antibody-ep1991y-bsa-and-azide-free-ab284737--immunocytochemistry-immunofluorescence-img437484.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 3 antibody [EP1991Y] - BSA and Azide free (ab284737)")
Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 3 antibody [EP1991Y] - BSA and Azide free (ab284737)
This data was developed using Anti-Dishevelled 3 antibody [EP1991Y] ab76081, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 cells labelling Dishevelled 3 with Anti-Dishevelled 3 antibody [EP1991Y] ab76081 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution at RT for 45 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) was used to counterstain tubulin at 1/200 dilution. Nucleus were visualized using DAPI.
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