JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB152161

Anti-Dishevelled 3 antibody - N-terminal

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal Dishevelled 3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DVL3 aa 1-200.

View Alternative Names

KIAA0208, DVL3, Segment polarity protein dishevelled homolog DVL-3, Dishevelled-3, DSH homolog 3

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 3 antibody - N-terminal (AB152161)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 3 antibody - N-terminal (AB152161)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling Dishevelled 3 protein at cytoplasm using ab152161 at a 1/500 dilution (Green).
phalloidin, a cytoskeleton marker, stained by phalloidin antibody at a 1/200 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

Western blot - Anti-Dishevelled 3 antibody - N-terminal (AB152161)
  • WB

Unknown

Western blot - Anti-Dishevelled 3 antibody - N-terminal (AB152161)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Dishevelled 3 antibody - N-terminal (ab152161) at 1/10000 dilution

All lanes:

HCT116 whole cell lysate at 30 µg

Predicted band size: 78 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dishevelled 3 antibody - N-terminal (AB152161)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dishevelled 3 antibody - N-terminal (AB152161)

Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft tissue, staining Dishevelled 3 using ab152161 at a 1/100 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DVL3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q92997

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Involved in the signal transduction pathway mediated by multiple Wnt genes.
See full target information DVL3

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:7241 PubMed39174557

2024

Alveolar epithelial cells mitigate neutrophilic inflammation in lung injury through regulating mitochondrial fatty acid oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Kuei-Pin Chung,Chih-Ning Cheng,Yi-Jung Chen,Chia-Lang Hsu,Yen-Lin Huang,Min-Shu Hsieh,Han-Chun Kuo,Ya-Ting Lin,Yi-Hsiu Juan,Kiichi Nakahira,Yen-Fu Chen,Wei-Lun Liu,Sheng-Yuan Ruan,Jung-Yien Chien,Maria Plataki,Suzanne M Cloonan,Peter Carmeliet,Augustine M K Choi,Ching-Hua Kuo,Chong-Jen Yu
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com