AB226210

Anti-DIXDC1 antibody

Name: Anti-DIXDC1 antibody (ab226210) | Abcam
Brand: Abcam Limited
SKU: AB226210
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal DIXDC1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DIXDC1 aa 600 to C-terminus.

View Alternative Names

CCD1, KIAA1735, DIXDC1, Dixin, Coiled-coil protein DIX1, DIX domain-containing protein 1, Coiled-coil-DIX1

2 Images

Supplier Data

Immunoprecipitation - Anti-DIXDC1 antibody (AB226210)

DIXDC1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226210 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226210 at 1 μg/ml.

Lane 1 : ab226210 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-DIXDC1 antibody (ab226210)

Predicted band size: 77 kDa

false

Supplier Data

Western blot - Anti-DIXDC1 antibody (AB226210)

All lanes:

Western blot - Anti-DIXDC1 antibody (ab226210) at 0.4 µg/mL

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 77 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human DIXDC1 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q155Q3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DIXDC1 also known as Dixin or Ccd1 functions as a cytoplasmic signal transducer involved in the regulation of the Wnt signaling pathway. The protein has a molecular weight of approximately 86 kDa. It is expressed in various tissues with higher expression levels in the central nervous system including the brain. The protein plays a significant role in cellular signaling and cytoskeletal organization impacting different cellular processes.

Biological function summary

DIXDC1 influences cellular dynamics by participating in cell migration and invasion. It acts as a stabilizing component in complexes with other proteins within signaling pathways. The protein interacts with Dishevelled (Dvl) proteins facilitating Wnt signaling. DIXDC1 also contributes to the modulation of the actin cytoskeleton thereby impacting cell morphology and motility.

Pathways

The role of DIXDC1 becomes evident within the canonical Wnt signaling pathway and cytoskeletal reorganization processes. The protein interacts with key components like Dishevelled proteins which are central to Wnt signaling. Moreover DIXDC1 links to the Ras-related C3 botulinum toxin substrate 1 (Rac1) pathway which impacts actin filament dynamics and cellular movement. This positions DIXDC1 as integral in processes governing cellular architecture and signaling.

Abnormal activity or expression levels of DIXDC1 can be associated with neurological disorders such as schizophrenia and cancer progression including glioblastoma. The dysregulation of DIXDC1 affects downstream interactions and pathways such as alterations in Rac1 activity which can contribute to the pathophysiology of these conditions. Understanding how DIXDC1 functions in disease contexts offers potential therapeutic insights.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Positive effector of the Wnt signaling pathway; activates WNT3A signaling via DVL2. Regulates JNK activation by AXIN1 and DVL2.

See full target information DIXDC1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 12:12755-12766 PubMed34898351

2021

Circular RNA circ_0000423 promotes gastric cancer cell proliferation, migration and invasion via the microR-582-3p/Disheveled-Axin domain containing 1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chao-Qun Huang,Ping Yang,Jiuyang Liu,Yin-Ping Wang,Dan-Dan Hao,Xiaojun Yang

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DIXDC1 antibody

Immunoprecipitation - Anti-DIXDC1 antibody (AB226210)

Western blot - Anti-DIXDC1 antibody (AB226210)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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