Rabbit Polyclonal DIXDC1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DIXDC1 aa 600 to C-terminus.
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Publications
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- Bioengineered 12:12755-127662021Circular RNA circ_0000423 promotes gastric cancer cell proliferation, migration and invasion via the microR-582-3p/Disheveled-Axin domain containing 1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao-Qun Huang,Ping Yang,Jiuyang Liu,Yin-Ping Wang,Dan-Dan Hao,Xiaojun YangPubMed 34898351
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human DIXDC1 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q155Q3
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Chicken | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey | Dilution info - | Notes - |
Target data
Function
Positive effector of the Wnt signaling pathway; activates WNT3A signaling via DVL2. Regulates JNK activation by AXIN1 and DVL2.
Alternative names
Dixin, Coiled-coil protein DIX1, DIX domain-containing protein 1, Coiled-coil-DIX1, DIXDC1, CCD1, KIAA1735
Recommended products
Rabbit Polyclonal DIXDC1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DIXDC1 aa 600 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human DIXDC1 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q155Q3
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DIXDC1 also known as Dixin or Ccd1 functions as a cytoplasmic signal transducer involved in the regulation of the Wnt signaling pathway. The protein has a molecular weight of approximately 86 kDa. It is expressed in various tissues with higher expression levels in the central nervous system including the brain. The protein plays a significant role in cellular signaling and cytoskeletal organization impacting different cellular processes.
- Biological function summary
DIXDC1 influences cellular dynamics by participating in cell migration and invasion. It acts as a stabilizing component in complexes with other proteins within signaling pathways. The protein interacts with Dishevelled (Dvl) proteins facilitating Wnt signaling. DIXDC1 also contributes to the modulation of the actin cytoskeleton thereby impacting cell morphology and motility.
- Pathways
The role of DIXDC1 becomes evident within the canonical Wnt signaling pathway and cytoskeletal reorganization processes. The protein interacts with key components like Dishevelled proteins which are central to Wnt signaling. Moreover DIXDC1 links to the Ras-related C3 botulinum toxin substrate 1 (Rac1) pathway which impacts actin filament dynamics and cellular movement. This positions DIXDC1 as integral in processes governing cellular architecture and signaling.
- Associated diseases and disorders
Abnormal activity or expression levels of DIXDC1 can be associated with neurological disorders such as schizophrenia and cancer progression including glioblastoma. The dysregulation of DIXDC1 affects downstream interactions and pathways such as alterations in Rac1 activity which can contribute to the pathophysiology of these conditions. Understanding how DIXDC1 functions in disease contexts offers potential therapeutic insights.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-DIXDC1 antibody (ab226210)
All lanes: Western blot - Anti-DIXDC1 antibody (ab226210) at 0.4 µg/mL
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 3min
Immunoprecipitation - Anti-DIXDC1 antibody (ab226210)
DIXDC1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226210 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226210 at 1 μg/ml.
Lane 1: ab226210 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
All lanes: Immunoprecipitation - Anti-DIXDC1 antibody (ab226210)
Predicted band size: 77 kDa
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